Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Preventive Medicine of Chinese People's Liberation Army》 2003-01
Add to Favorite Get Latest Update

IDENTIFICATION OF SHIGELLA BY RANDOM AMPLIFIED POLYMORPHIC DNA METHOD

LI Junwen CHAO Fuhuan SHI Xiuquan WANG Xinwei ZHENG Jinlai SONG Nong JIN Lianqun JIN Min (Institute of Health and Environmental Medicine,Academy of Military Medical Sciences,Tianjin,300050)  
Objective:To deveolp a method to identify the Shigella in water or food.Methods:First,the specific PCR was used to detect the isolated bacteria whether they are the Shigella, and then random amplified polymorphic DNA,(RAPD) was used to identify the bacteria.Results:The bacteria (Shigella and EIEC) with positive results of ipaH-primer PCR were chosen to be classified by the level of genes with RAPD. The random primer S 756 of the most effective amplification was selected to distinguish four species of Shigella and one of EIEC. By analysis of fingerprinting and the data, the results indicated that the same strains amplified the similar stripes, while different strains even different subtypes of the same strain also revealad some disparities.Conclusion:RAPD may be used to identify the Shigella in water or food.
【Fund】: 全军医药卫生基金课题项目 (No .96M0 4 8)
【CateGory Index】: R378
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 4 Hits
1 GUO Ai-ling et al(College of Food Science and Technology,Huazhong Agricultural University,Wuhan,Hubei 430070);Establishment of RAPD Identification Method for Shigella[J];Journal of Anhui Agricultural Sciences;2008-11
2 HUANG Bao-hua,CHEN Qing-sen*,PANG Guang-chang(The Tianjin Key Laboratory of Food Biotechnology, Tianjin University of Commerce, Tianjin 300134, China);Developing Status of Fast Detection Technique and Study on Shigella[J];Food Science;2004-11
3 LIN Xiao-li,LAI Wei-hua,ZHANG Li-li (State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China);Recent Advances in Detection of Shigella Species in Food[J];Food Science;2009-15
4 Ge Cuicui Zhong Qingping (College of Food Science, South China Agricultural University, Guangzhou 510640);Purification of Anti- shigella IgY and Establishment of Indirect ELISA for Detecting Shigella[J];Journal of Chinese Institute of Food Science and Technology;2006-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 XU Yan-li et al(College of Food Science and Engineering,Ocean University of China,Qingdao,Shandong 266003);Research Progress on Detection and Biological Prevention of Aflatoxins[J];Journal of Anhui Agricultural Sciences;2007-32
2 GUO Ai-ling et al(College of Food Science and Technology,Huazhong Agricultural University,Wuhan,Hubei 430070);Establishment of RAPD Identification Method for Shigella[J];Journal of Anhui Agricultural Sciences;2008-11
3 PEI Hai-long et al (Academy of Life Science,Northwest University,Xian,Shaanxi 710069 );Determination on Isopropyl Salicylate Anti-bacterial Activity in vitro[J];Journal of Anhui Agricultural Sciences;2008-34
4 DONG Jie et al(School of Culinary Tourism,Yangzhou University,Yangzhou,Jiangsu 225001);Observation of the Control of Aeromonas in the System of Drunken Process[J];Journal of Anhui Agricultural Sciences;2008-36
5 CHEN Xiao-ling et al(Academy of Life Science,Northwest University,Xian,Shaanxi 710069);Determination of Ethyl Salicylate Anti-bacterial Activity in vitro[J];Journal of Anhui Agricultural Sciences;2009-02
6 Zhang Xiaofei et al(Animal Husbandry and Veterinary Research Institute. Anhui Academy of Agricultural Sciences,Hefei 230031);Studies on the Detection for Duck Hepatitis Virus by Dot Immuno-gold Filtration Assay[J];Journal of Anhui Agricultural Sciences;1997-04
7 Ding Ye-rong,Yang Jie ,FanYu-zhen,Shi Quan,et al Liu' an Center for Disease Control and Pre vention Anhui 237008;Discovery and study on pathogenic E.coli of producing H_2S[J];Journal of Anhui Health Vocational & Technical College;2003-05
8 CHENG Bang-ning~(1),WU Er-bin~(2)(1.China Tiesiju Civil Engineering Group Center Hospital,Hefei Anhui,230023,China;2.China Tiesiju Civil Engineering Group Construct Company Hospital,Hefei Anhui,230023,China);Drug Resistance Analysis of S.pneumoniae[J];Anhui Journal of Prevent Medicine;2006-04
9 Zhang Xunhai(Dept. of Animal Science in Anhui Agrotechnical Teachers College, Fengyang 233100)Chen Puyan; Cai Baoxiang(College of Veterinary Medicine in Nanjing Agricultural University Nangjing, 210095);Use of Gene Probe dot-blot Hybridization Technique for Specific Detection Serotype 1 Marek's Disease Viruse[J];JOURNAL OF ANHUI AGROTECHNICAL;1997-03
10 Li Jie;Zhang Qiying; Guo Cai;Liu Jun(Department of Clinical Laboratory,Affiliated Hospital of Bengbu Medical College,Anhui 23 3004);Evaluation of PCR detection of Neisseria gonorrhoeae[J];ACTA ACADEMIAE MEDICINAE BENGBU;1996-01
China Proceedings of conference Full-text Database 1 Hits
1 LIU Zi-kui~1 YAO Guo-min~2 WU Ye~1 HU Xing-ping~1 ZHU Zhong-ping~1 (1.Hunnan AgricuLturaL University AnimaL PharmaceuticaL co.;Ltd.Changsha Hunan,410128;2.Huaihua VocationaL and TechnicaL CoLLege,Huaihua Hunan,418000;China);Observation on Immunopotentiation of Compound AstragaLus PoLysaccharides Injection as Immunoadjuvant[A];[C];2008
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 FAN Bin, LIU Bang, LI Kui (Lab of Molecular Biology and Breeding, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China ) \;;Random Amplified Microsatellite Polymorphisms(RAMPs) and Its Application[J];Journal of Yellow Cattle Science;2002-02
2 MA Yu-hong, MA Zhi-jie (Institute of Animal Husbandry of Qinghai Academy of Animal Husbandry and Veterinary, Xining, Qinghai 810016, China);RFLP and PCR-RFLP Markers and Genetic and Breeding Research in Yak[J];China Cattle Science;2006-05
3 XIAO Yu tang\+1,ZHANG Jing jing\+1,WU Ming\+1,LIU Hui\+2,ZHANG Dong\+2,XU Jianhua\+2 (1.Wastewater Treatment Engineering Group, Dalian Institute of Chemical Physics,The Chinese Academy of Sciences,Dalian 116023,China;2.School of Environmental Engine;POLLUTION OF WATER RESOURCE AND THE SAFETY OF DRINKING WATER[J];Resources and Enuironment In The Yangtza Basin;2001-01
4 Guofu Su;Genetic Determinant of Shigella Pathogenicity and Strategies for Development of Potential Candidate Vaccines[J];;1998-03
5 BAI Li, QIAN Jin-hu, WANG Jin (Department of Microbiology& Immunology,Dali Medical College,Dali 671000, China);Purification of Mouse IgG from Ascites Fluid and Serum by Caprylic Acid and Ammonium Sulfate[J];Journal of Dali Medical College;2000-04
6 Chen Yongjiu Zhang Yaping (Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming 650223);STUDIES ON FACTORS IN RANDOM AMPLIFIED POLYMORPHIC DNA ANALYSIS[J];ZOOLOGICAL RESEARCH;1997-02
7 XIANGQian 1 ,YUShou-yi 2 ,WANGHong 2 ( 1 Departmentof MedicalTeachingandResearch,NanfangHospital,2 Departmentof Epidermiology,FirstMilitaryMed-icalUniversity,Guangzhou510515,China);gyrA gene mutations in quinolone-resistant Shigellae flexneri[J];Journal of First Military Medical University;2001-12
8 NI Hong bo 1 , WANG Xing long 2 (1.Animal Science and Technology College of Heilongjiang August First Agriculture Universety ,Mishan,158308,China; 2. Department of Animal Medicine,the Quartermaster University of PLA,Changchun,130062,China);Technique of RAPD and its Application to Typing of Streptococcus[J];Progress In Veterinary Medicine;2002-02
9 CHEN Jun-feng~1,2,YU Chun-fang~1,HUANG Bing~2,HAN Jing-fang~1,2(1.College of Animal Science and Technology,Southwest University,Chongqing,400715,China;2.Shanghai Institute of Domestic Animal Parasitology,Chinese Academy of Agricultural Sciences,Key Laboratory of Animal Parasitology,Ministry of Agriculture,Shanghai,200232,China);Application of Molecular Marker Technology in Classification and Identification of Parasites[J];Progress in Veterinary Medicine;2006-05
10 JIANG Zi feng, LIN Nai quan, XU Mei(Biological Control Research Institute, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China);A review on some technical problems in RAPD application[J];Journal of Fujian Agricultural University(Natural Science);2002-03
【Secondary References】
Chinese Journal Full-text Database 7 Hits
1 LI Xiao-hua,YANG Xiao-yan,DAI Ai-ling(Department of Biological Science and Engineering,Longyan University,Longyan,Fujian 364000,China);Development and application of chicken egg yolk antibody against Candida albicans[J];;2006-06
2 XIAO Min1,2,YI Yong2,MAO Xuhu1,LUO Ping1,NIE Leng2,JIA Liping2,WEI Ping2,WANG Hui2,JING Hua2,ZOU Quanming1 1. Department of Clinical Microbiology & Clinical Immunology,College of Medical Laboratory,Third Military Medical University,Chongqing 400038,China; 2. Department of Clinical Laborabody,the 306th Hospital of PLA,Beijing,100101,China;Establishment of nephelometric immunoassay combined with immunomagnetic separation for the rapid automatic quantitative detection of Shigella flexneri[J];Immunological Journal;2009-03
3 ZHAO Li-hua,ZHOU Yong,WAN Cheng-song(Department of Microbiology,School of Public Health and Tropical Medicine Southern Medical University,Guangzhou 510515,China);Detection of Shigella Gene ipaH Using Molecular Beacons[J];Journal of Tropical Medicine;2006-05
4 LIN Xiao-li,LAI Wei-hua,ZHANG Li-li (State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China);Recent Advances in Detection of Shigella Species in Food[J];Food Science;2009-15
5 Chen Wei 1,2,Li Zhengguo 1,2,Yang Ping 1,2,Yang Yingwu 1,2,Wang Guoming 1,2 1(Genetic Engineering Research Center,College of Bioengineering,Chongqing 400030,China)2(Key Laboratory of Functional Gene and Regulation Technologies under Chongqing Municipal Education,Commission,Chongqing University,Chongqing 400030,China);The Study on Rapid DNA Extraction Method of Shigella from Meat Products[J];Food and Fermentation Industries;2009-05
6 Xu Wei Liu Jun Li Sufang (College of Life Sciences, China Jiliang University, Hangzhou 310018);The Development of Multiplex PCR Technique for Detection of Listeria Monocytogenes and Shigella[J];Journal of Chinese Institute of Food Science and Technology;2009-01
7 JIANG Da-wei,LI Kai,XU Lan-ju*,WANG Chuan-qing,ZHANG Yu-hong(Engineering College of Animal Husbandry and Veterinary Science,Henan Agricultural University,Zhengzhou 450002,China);Development of the agglutinating antigen and standard serum of Shigella from chicken[J];Chinese Journal of Veterinary Science;2009-06
China Proceedings of conference Full-text Database 2 Hits
1 Liu Hongying Yang Xia Chen Lu Xu Lanju Dong Jiacai Liu Kuang Wu Qing Xue Shuang Wang Chuangqing(Henan Agricultural University,Zhengzhou,450002);The Large Virulence Plasmids transferred and Artificial Infection of White Mice[A];[C];2008
2 Liu Hongying Yang Xia Chen Lu Xu Lanju Dong Jiacai Liu Kuang Wu Qing Xue Shuang Wang Chuangqing(Henan Agricultural University,Zhengzhou,450002);Artificial Infection of Chickens with Shigella Isolates from Human[A];[C];2008
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved