Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Preventive Medicine of Chinese People's Liberation Army》 2006-05
Add to Favorite Get Latest Update


LUO Li-min,LI Ming,WANG Wen-jing,RUI Rong-yu,CHEN Yao(Institute of Tropical Diseases,Southern Medical University,Guangzhou 510515,China)  
Objective To investigate the genetic polymorphism of 99 Brucella strains isolated in China,so as to provide the basis for ribotyping and diagnosis of Brucella infection.Methods Chromosomal DNA of Brucella was digested with Eco RI,and separated by agarose gel electrophoresis and Southern blotting.Then it was transferred into a nylon filter and hybridized with 16 s rDNA which was labeled with digoxigenin-dUTP.Hybrid was detected by enzyme immunoassay.Compare the fingerprint of 16 s rRNA in different Brucella strains.Results 16 s rRNA fingerprint of different Brucella strains showed high polymorphism.99 Brucella strains detected belonged to 17 ribotypes(RT) according to the numbers and locations of strips on 16 s rRNA fingerprint.RT 15,as the most predominant ribotype,comprised 26.3% Brucella strains.The location of other ribotypes was scattered,some ribotype was only found in certain strain.Conclusions Brucella strains in China showed genetic polymprphism and clonal diversity,suggesting that the epidemic of Brucella is complicated.
【Fund】: “973”课题(No.2002CB513206)
【CateGory Index】: Q933
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 3 Hits
1 Sun Tianzhi,Zhang Qinghua,Sun Zhongqiang.In Inner Mongolia Insitute of the Prevention and Treatment of Endemic Disease,Huhhot 010020.;Analyse on the Epidemiological Characteristics of Brucellosis and in Inner Mongolia Through the Observation of the Change among Brucella Species[J];Chinese Journal of Epidemiology;1998-05
2 WEI Jian-chun, YU Dong-zheng, HAI Rong. Institute for Infectious Diseas e Control and Prevention, Chinese Center for Disease Control and Prevention, Bei jing 102206, China;The geographical distribution of ribotypes of Yersinia pestis in China[J];Chinese Journal of Epidemiology;2003-11
3 Duan Guang-cai,Gao Shou-yi,QI Guo-ming ,et al(Institute of Epidemiology and Microbiology,Chinese Academy of Preventive Medicine,Beijing 102206);COMPARISONS ON THE MOLECULAR CHARACTERISTICS OF VIBRIO CHOLERAE O139 ISOLATED FROM CHINA,ONDIA AND BANGLADESH[J];CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY;1994-06
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 Liu Zhencai,Hai Rong,Li Fuzhong,et al(Chinese Base for Control of Plague and Brucellosis,Baicheng Jilin ,137000);The discovery and study of Microtus fuscus Nature Plague Foci in Qinghai-Tibet Plateau[J];Chinese Journal of Control of Endemic Disenaces;2001-06
2 Ji Shuli,et al;A Study on Typing of Y.Pestis in China and Ecologico-epidemiological Significance[J];Chinese Journal of Endemiology;1987-05
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved