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《Journal of Preventive Medicine of Chinese People's Liberation Army》 2009-04
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ANTI-ENTEROVIRUS EFFECTS OF AQUEOUS EXTRACT FROM SPATHOLOBUS SUBERECTUS DUNN IN VITRO AND IN VIVO

PANG Ji,GUO Jin-peng,JIN Min,SHEN Zhi-qiang,WANG Xin-wei,QIU Zhi-gang,LI Jun-wen(Institute of Hygiene and Environmental Medicine,Academy of Military Medical Sciences,Tianjin 300050,China)  
Objective To investigate the anti-enterovirus effects of the aqueous extract(AE)from Spatholobus suberectus Dunn(SSD)in vitro and in vivo for developinga new anti-enterovirus agent.Methods In vitro:the antiviral effects on enteroviruses,including Coxsackie virus B3,Coxsackie virus B5,Echo virus 9,Echo virus 29,and Polio virus I in Vero cells were studied by CPE and MTT methods.In vivo:the effects of SSD aqueous extract on cardiac index(the ratio of heart weight to body weight),and survival rate of BALB/c mice after the animal infected by intraperitoneal injection of Coxsackie virus B3 to induce viral myocarditis were examined.Finally,The inhibitory effects of SSD on Coxsackie virus B3 in vitro and in vivo by measuring mRNA expression with RT-PCR were observed.Results SSD aqueous extract showed inhibitory activities against all five species of enterovirus in various percentages in vitro and in vivo depending upon the concentrations.The median effective concentration(IC50,50% inhibition concentration)of SSD aqueous extract against replication of Coxsackie virus B3,Coxsackie virus B5,Echo virus 9,Echo virus 29,and Polio virus I were 60.8,47.1,14.8,65.5,and 29.1 μg/mL in Vero E6 cells respectively.The therapeutic index(TI=CC50/IC50)of AE were 4.1,5.3,16.9,3.8,and 8.6 respectively.Meanwhile the viral myocarditis induced by Coxsackie virus B3 was inhibited significantly by AE and the mortality rate was decreased.Conclusion Spatholobus suberectus Dunn aqueous extract has inhibitory effect on enterovirus in vitro and in vivo.
【Fund】: 国家“863”项目课题(No.2006AA06Z408)
【CateGory Index】: R285
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