The Phenotype Reversion of Human Myelogenous Leukemic Cells Caused by Human Cord Blood Cytoplasm Treated with TCM Drugs for Clearing Away Heat and Nourishing Yin

Zhang Hongjun(张洪钧), Chen Xinyi(陈信义), Sun Yingli(孙颖立)(Dongzhimen Hospital, Beijing University of Traditional Chinese Medicine, Beijing 100700)  

In this experiment the human cord blood hematopoietic cell (HCBHC) was used to cultivate a kind of cell whose phenotype is contrary to that of the cell with acute leukemia by a TCM drug culture method. First, HCBHC was cultivated with TCM drugs for clearing away heat and nourishing yin (CHNY), and then, its cytoplasm was used to convert the phenotype of the cell with acute leukemia. The results showed that TCM drugs for CHNY, could decrease HCBHC multiplication rate, increase HCBHC differentiation rate, and cause the readjustment and recombination of HCBHC hematopoietic modulators; and the phenotype of HL60 cells could be converted by HCBHC cytoplasm cultivated with TCM drugs for CHNY, manifesting mature mononuclear macrophage differentiation, declined division rate, inhibition of multiplication, and apoptosis.

【Key Words】： HL60 Cell Differentiation Traditional Chinese Drugs Human Cord Blood Cytoplasm
【CateGory Index】： R285

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