Effects of Cholic Acid and Jasminoidin on the Levels of Cerebral TNF-α, IL-1 β and ICAM-1 in the Rats Undergone Cerebral Ischemia
Zhu Xiaolei , Zhang Na , Li Pengtao , et al. (The Teaching and Research Section of Pathology, Beijing University of Traditional Chinese Medicine, Beijing 100029)
Objective To observe the effects of cholic acid and jasminoidin in combination on the levels of cerebral TNF-α, IL-1 β, ICAM-1 and serum NSE in the rats with persistent focal cerebral ischemia, in order to investigate the links at which cholic acid and jasminoidin act to inhibit the cascade of the cerebral ischemic inflammatory injury and to investigate the mechanism of the effects produced by cholic acid and jasminoidin in combination. Methods Adult male SD rats were randomly divided into the sham operation group, model group, cholic acid group, jasminoidin group, cholic acid plus jasminoidin group, and Xueshuantong group. The rat model of focal cerebral ischemia was induced by persistent middle cerebral artery occlusion for 12 and 14 hours, respectively, by a thread ligation method. RIA was used to detect the levels of TNF-α and IL-1 β in the cerebral homogenate and the level of NSE in the serum. ELISA was used to detect the level of ICAM-1 in the cerebral homogenate. Results The levels of TNF-α, IL-1 β, ICAM-1 and NSE were found increased to different degrees, respectively, under cerebral ischemia for both 12 and 24 hours. Both cholic acid and jasminoidin could markedly inhibit the increase of TNF-α and IL-1 β under both the 12-hour and 24-hour cerebral ischemia; in addition, cholic acid could also inhibit the increase of NSE under the 12-hour cerebral ischemia; but both cholic acid and jasminoidin showed no obvious effects on the levels of ICAM-1 and of NSE under the 24-hour cerebral ischemia. However, the coadministration of cholic acid and jasminoidin could not only markedly inhibit the decrease of TNF-α and IL-1 β under both the 12-hour and 24-hour cerebral ischemia, but also markedly inhibit the increase of ICAM-1 under both the 12-hour and 24-hour cerebral ischemia and the increase of NSE under 12-hour cerebral ischemia. Conclusion Cholic acid and jasminoidin and their coadministration possess good effects for repressing the expressions of inflammatory cytokines and adhesive molecules under cerebral ischemia, hence reducing the inflammatory injury. The coadministration of cholic acid and jasminoidin shows particularly good effects for interfering the inflammatory process at different times and different links and for repressing the cascade of the inflammatory injury due to cerebral ischemia, hence inhibiting the damage to neurons.
【Fund】： 国家重点基础研究发展规划项目 (973 )课题 (No .G19990 5 5 40 4)
【CateGory Index】： R285.5
【CateGory Index】： R285.5