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Intervention effect of Yijing Formula on sperm apoptosis in mouse model of oligoasthenospermia induced by CTX

LUO Shao-bo1,HU Hai-xiang1,JIA Jin-ming2(1 Center of Andrology,General Hospital of Air Force,Beijing 100142;2 Department of Andrology,Guang'anmen Hospital,China Academy of Chinese Medical Scienes)  
Objective To investigate the influence of Yijing Formula(with the actions of tonifying kidney and replenishing essence,and tonifying and activating blood) on sperm apoptosis in mouse model of oligoasthenospermia(OAT) induced by cyclophosphamide(CTX).Methods Male Kunming mice(n=40) were randomly divided into the control group(CG),model group(MG),small-dose treatment group(SG) and large-dose treatment group(LG).CG was given intraperitoneal injection of normal saline solution(60 mg/kg) once a day for 5 days,and the MG,SG and LG were given the intraperitoneal injection of CTX(60 mg/kg) once a day for 5 days.On the 6th day SG and LG were given orally Yijing Formula according to the body weight(double or 5 times of human common dosage respectively) once a day for 34 days,and MG was given isopyknic normal saline solution once a day for 34 days.CG was fed normally.On the 40th day all mice were given routine analysis of seminal fluid.The sperm apoptosis rate was detected by using flow cytometer after FITC-AV/PI staining.Results In CG,MG,SG and LG,the sperm count was,respectively,5.20±1.34,1.73±0.03,2.08±0.01 and 3.31±0.56(×109 L-1);sperm motility was,respectively,(14.49±0.30)%,(6.64±1.88)%,(11.99±1.01)% and(19.40±3.13)%;and motility rate was,respectively,(68.39±15.13)%,(39.96±4.89)%,(62.28±4.43)% and(73.61±5.05)%.The sperm count,sperm motility and motility rate were significantly lower in MG than those in CG(P0.05),and those in LG were significantly increased after the intervention of Yijing Formula.There was a significant difference between LG and MG(P0.05),but no difference in the sperm motility and motility rate between LG and CG.The sperm apoptosis rate in four groups was,respectively,(8.38±0.45)%,(14.84±1.22)%,(13.25±1.83)% and(8.35±2.1)%.The sperm apoptosis rate was significantly higher in MG than that in CG(P0.05).After the intervention of Yijing Formula the sperm apoptosis rate was significantly lower in LG than that in MG(P0.05),but no significant difference compared with CG.Conclusion The intraperitoneal injection of CTX can increase sperm apoptosis and decrease sperm count,motility and motility rate in mice.The intervention of large-dose Yijing Formula can decrease sperm apoptosis rate and increase sperm count,motility and motility rate aiming at cure OAT.
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