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《Journal of Jinzhou Medical Coilege》 1990-02
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THE EFFECT OF EPIMEDIUM POLYSACCHARIDE(EPS) AND OTHERE THREE DRUGS ON HUMAN INTERLEUKINE 2(IL_2) ACTIVITY——MTT COLORIMETRIC ASSAY USING THYMOCYTES OF MICE

Wang Lunan et al(Department of Microbiology, Jinzhou Medical College)  
The effects of Epimedium Polysaccharide(EPS), Grifola Polysacchari de (GPS), Carboxymethyl-Pachmaran(CMP) and Microelement zinc on IL_2 production of normal human peripheral blood mononuclear cells(PBMC) were reported in the paper. IL_2 activity was assayed by MTT colorimetric assay using thymocytes of mice as target cells. The results showed that IL_2 production was remarkbly increased by 100μg/ml, 1000μg/ml of EPS and was significantly inhibited by 2500μg/ml, 5000μg/ml of EPS The significant augmentation on human IL_2 production was manifested when mixture of GPS with CMP of certain concentration. The zinc of 0.153～765μ mol/L concentrations possessed significant incresing effect.The present study shows that increase of every drugs on IL_2 production was not directly effect of drugs inhibiting effet of EPS of high concentration was not also cytotoxic effect. The MTT colorimetric assay using thymocytes of mice as target cells was established and compared with 3~H-TdR incorporation assay. It showed that the results determined by MTT colorimetric assay were incomformity with those obtained by 3~H-TdR incorporation assay.
【Key Words】： Epimedium Polysaccharide Grifola Polysaccharide Carboxymethl-Pachmaran Microelement Ziuc Interle uKine2 MTT Colorimetric assay Thymocyte
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