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《Journal of Liaoning Medical University》 2009-01
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Determination of Total Lignanoids in Fructus Arctii's Extract by Ultraviolet Spectrometry

YAN Ting,WANG Peiqi,GUO Weiying(Liaoning Medical University,Jinzhou 121001 China)  
Objective To establish a determination method for the content of total lignanoids in Fructus Arctii's Extract.Methods According to total lignanoids and arctiin in the same peak absorption,using arctiin as reference substance,the absorption value of the sample at 280 nm was measured to calculate content directly.Results The calibration curve of the absorbency has linear relation with the concentration of arctiin in the range of 0.01 804~0.0 902 mg/mL(r=0.9 999).The average recovery of arctiin was 98.6%,and the RSD value is 1.7%.The content of total lignanoids(in the amount of arctiin) per gram in extract is at least 800 mg.Conclusions Method of determining on the total lignanoids through ultraviolet spectrometry is feasible,stable and reliable as well as precise.
【CateGory Index】: R284
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