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Study on the Prevention of Cataract in Rat Lens from Dendrobium nobile Lindl.Extractives in vitro

WEI Xiao-yong,LONG Yan,ZHAN Yu-jian,LI Xi-chan,XU Qin,XU Chuan-lei(Guangzhou University of TCM,Guangzhou 510405,China)  
Objective To probe into the inhibitory effects of the total alkaloids and polysaccharides on cataract lens in vitro from extracted of Dendrobium nobile.Methods The total alkaloids and polysaccharides were extracted from Dendrobium nobile;Wistar rats' crystalline lenses were cultured in DMEM medium and divided into 7 groups. The opacity of each crystalline lens was observed and recorded respectively with different time.Finally,MDA of each lens was measured with the kits respectively.Results The content of water-solubility protein and GSH and the activity of T-SOD were increased in the groups of total alkaloids and polysaccharides,and the activity of MDA was decreased as well.Conclusion The total alkaloids and polysaccharides of Dendrobium nobile had both inhibited cataract lens which caused by oxidative damage in vitro, and the effect of total alkaloids was better than that of polysaccharide.
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