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《Research and Practice on Chinese Medicines》 2010-06
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Determination of Abrine and Hypahorine in Abrus mollis Hance. by HPLC

HUANG Ping,MAI Qiu-liang,MENG Xiao-fang(Guangxi Institute of Traditional Medical and Pharmaceutical Sciences,Nanning 530022,China)  
Objective To establish a method for the determination of abrine and hypaphorine in Abrus mollis Hance.Methods An HPLC method was performed on Lichrospher C18 column(250 mm × 4.6 mm,5 μm) at room temperature,with water-methanol-acetonitrile-glacial acetic acid-triethylamine(82:13:5:0.2:0.3) as mobile phase.The flow rate was 1.0 mL·min-1,and the detection wavelength was set at 220 nm.Results 14 samples had been determinated.Abrine and hypaphorine had been basically isolated.Conclusion The method is simple,accurate and reproducible.It can be used for quality control of Abrus mollis Hance.
【Fund】: 广西科技攻关项目(编号:0424008-2A)
【CateGory Index】: R284.1
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