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《Acta Entomologica Sinica》 1985-04
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STUDIES ON THE ISOZYMES OF HONEYBEES

LI SHAO-WEN MENG YU-PIN J. T. CHANG (Department of Biology, Peking University)LI JU-HUAI (Peking Academy of Agricultural Sciences)  
Electro-focusing PAGE method has been used to study esterase isozyme variations in two species of honeybees, Apis mellifera ligv.stica and A. cerana. There are obvious differences between the species, and also certain differences in different castes and developmental stages of A. mellifera.Zymograms show typically nine bands and are divided into four zones, Est Ⅰ, Est Ⅱ, Est Ⅲ and Est Ⅳ. Est Ⅳ is polymorphic in A. mellifera ligustica. Est Ⅳ activity can not be detected in young white-eyed pupae, but it increases to maximum in old black-eyed pupal phases.
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