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《Acta Entomologica Sinica》 1991-02
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YANG GUAN-HUANG WANG RUI-WU(Institute of Apicultural Research,Chinese Academy of Agricultural Sciences,Beijing 100093)DU ZHI-LAN(Department of Biology,Beijing University,Beijing 100871)  
The in vitro hypopharyngeal gland (HP) activity of the workers of Apis cerana cerana (A.c.c.) of different age and at different seasons was investigated with short-term radioche-mical assay.The difference of the HP activities between A.c.c.and Apis mellifera ligustica (A.m.l.) was noticeable.The results are as follows:1.The incorporation of 3H-leucine by isolated HP and the portion secreted from the HP into the incubation medium were measured at different periods.It was seen that the rate of incorporation of 3H-lencine was almost linear over a period of 6 hours 2.During spring or summer,the activity of full-grown HP of the A.c.c workers 7 days old was the highest while in the A.m.l.workers it was highest when they were 10 days old.8.In winter,the HPs of overwintering A.c.c.workers are hy-penrophied,but the glands isolated front such bees were found to display low activity In the early spring when the colony started to breed,the HP of overwintering A.c.c,workers showed conspicuous variation in activity
【Fund】: 国家自然科学基金
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