Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Entomologica Sinica》 2003-03
Add to Favorite Get Latest Update

Modification and application of a high performance liquid chromatography method to separate juvenile hormones and their metabolites

OUYANG Ying-Chun1, LI Sheng 2*(1. Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China; 2. Department of Biological Sciences, Illinois State University, Normal, IL 61790-4120, USA)  
Juvenile hormones (JH) Ⅰ,Ⅱ and Ⅲ can be effectively separated by traditional normal phase high performance liquid chromatography (NP-HPLC), but their metabolites and analogues can not be clearly separated by this method. We have recently modified a method of reverse phase high performance liquid chromatography (RP-HPLC). This modified method can be used to separate not only different juvenile hormones, but also a variety of juvenile hormone metabolites and analogues, qualitatively and quantitatively. Trace amounts of radiolabeled juvenile hormone, which was synthesized and secreted by the in vitro incubated corpora allata (CA), could be separated by both NP-HPLC and RP-HPLC methods. Moreover, the RP-HPLC can be used to separate radiolabeled juvenile hormone metabolites and analogues, in both in vivo and in vitro conditions, as well as to measure juvenile hormone levels in the hemolymph.
【Fund】: 中国科学院知识创新工程重要方向项目 (KSCX2 - SW -10 5 )
【CateGory Index】: Q966
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 8 Hits
1 JI Bao-zhong*1,LIU Shu-wen2,TIAN Ling1,GUO Tong-bin3,JIN Feng1,GAO Jie1 (1.Nanjing Forestry University,Nanjing 210037;2.Management Office of Sun Yatsen's Mausoleum,Nanjing,210014;3.Forestry Station of Xuzhou,Jiangsu Province,Xuzhou,Jiangsu 221004);Progress in Research on Analytical Methods of Juvenile Hormone[J];Journal of Analytical Science;2007-01
2 Qian Minghui 1,2 Fan Junxiang 1 Qin Changsheng 1 (1. Guangdong Forest Research Institute, Guangzhou, 510520; 2. South China Agricultural University);Titres of Juvenile Hormone in Monochamus alternatus (Hope) by HPLC[J];Forestry Science and Technology;2004-02
3 PANG LING-YUN, DUAN YU-FENG, CHEN JIN-PING, YAO LI (DEPARTMENT OF FOOD ENGINEERING,SHAANXI NORMAL UNIVERSITY,XI′AN,SHAANXI 710062,CHINA);FUNCTIONAL INGREDIENTS OF GRASSHOPPER AND THEIR DEVELOPING PROSPECT[J];Journal of Economic Animal;2004-01
4 CAO Cheng-quan1,NIE Shu-xia2,CHEN Shen-zhi1,YE Bao-hua1,LIU Yu-sheng1(1.Shandong Agricultural University,College of Plant Protection,Tai′an 271000,China;2.Farming Exploitation Center of Center Borough,Jining,Shandong Province,Jinjing 272100,China);The Recyclical Exploitation and Use of Grasshopper[J];Journal of Economic Animal;2007-02
5 NING Chang-Ming1,2,WU Kong-Ming2,GAO Xi-Wu1,CHENG Hong-Mei3 (1. Department of Entomology,China Agricultural University,Beijing 100193,China; 2. State Key Laboratory for Biology of Plant Disease and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China; 3. Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China);Cloning of cDNA-encoding juvenile hormone epoxide hydrolase in Helicoverpa armigera(Hbner) and its expression in E. coli[J];Journal of Environmental Entomology;2009-02
6 LI Sheng~(1,2), JIANG RongJing~2, CAO MeiXun~2 (1. Department of Biological Sciences, Illinois State University, Normal, IL 61790, USA; 2. Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China);Metabolism of juvenile hormone[J];Acta Entomologica Sinica;2004-03
7 QIAN Ming-Hui1,2,REN Shun-Xiang2,HU Qiong-Bo2(1.Department of Forestry Protection,Guangdong Forestry Research Institute,Guangzhou 510520,China;2.Department of Entomology,College of Natural Resource & Environmental Science,South China Agricultural University,Guangzhou 510642,China);Reproduction,development and host relationship of aphelinids[J];Chinese Bulletin of Entomology;2007-04
8 YAO Jie~1,YAO Shi-hong~2(1.Guiyang Food & Biotechnology Research Institute,Guiyang,Guizhou 550002,China;2.School of Geography and Biology,Guizhou Normal University,Guiyang,Guizhou 550001,China);The development and utilization of grasshopper resource in Guizhou[J];Journal of Guizhou Normal University(Natural Sciences);2006-01
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 LIU Jian-tao et al (School of Life Science,Jiangxi Normal University of Science and Technology,Nanchang,Jiangxi 330013);Research Progress in the Juvenile Hormone Application and its Analogs[J];Journal of Anhui Agricultural Sciences;2006-11
2 Zou Yunding Wang Shihuai Wang Hongfa (Agricultural College of Anhui, Hefei, Anhui);STUDY ON THE ENERGY SUBSTANCES FOR MIGRATION OF ARMY WORM, LEUCANIA SEPARATA WALKER[J];Acta Zoologica Sinica;1984-04
3 ZHAO Zhong Ming LU Jian DAI Zhuo Hua ** (College of Life Sciences,Peking University,Beijing 100871,China);GENETIC DIFFERENTIATION WITHIN DROSOPHILA AURARIA SPECIES COMPLEX REVEALED BY RANDOM AMPLIFIED POLYMORPHIC DNA(RAPD)[J];Acta Zoologica Sinica;2001-06
4 WEN Jing, LIU Di, JIN Zonglian (Beijing Laboratory of Bioactive Substances and Functional Foods, Beijing Union University,Beijing\ 100083, China);Advances on the Study of Lovastatin Identification Methods[J];Journal of Beijing Union University;2003-03
5 Jiang Xinfu ;Liu Yueqiu ;Luo Lizhi; Huyi (Institute of Plant Protection Chinense Academy of Agrcultural Sciences , Beijing 100094);Effects of High Temperature on the ImmatureStages of The Oriental ArmywormMythimna SeparataWalker[J];JOURNAL OF BEIJING AGRICULTURAL COLLEGE;1998-02
6 Yun Zihou Yang Yifan Department of Chemistry Jiang Zaijie Chen Xiang Department of Biology:Beijing Normal University,100875,Beijing;ANALYSIS OF JUVENILE HORMONE IN HAEMAPHYSALIS LONGICORNIS BY HPLC[J];Journal of Beijing Normal University(Natural Science);1992-02
7 Zhang Shulin; Jiang Zaijie (Department of Biology, Beijing Normal University, 100875, Beijing, China);STUDIES ON THE SITE OF ECDYSTEROID SECRETION OF THE IXODIC TICK HAEMAPHYSALIS LONGICORNIS[J];JOURNAL OF BEIJING NORMAL UNIVERSITY(NATURAL SCIENCE);1997-04
8 Gai Yu; Xu Rumei; Richard Stouthamer; Joop C. van Lenteren(College of Life Sciences, Beijing Normal University, 100875, Beijing, China;Laboratory of Entomology, Wageningen University and Research Center The Netherlands);A MULTIPLEX PCR TO DETECT ENCARSIA FORMOSA HOST-FEEDING ON BEMISIA ARGENTIFOLII[J];JOURNAL OF BEIJING NORMAL UNIVERSITY(NATURAL SCIENCE);1999-03
9 Zhang Long ;Yan Yuhua(Department of Plant Protection, Beijing Agricultural University, Beijing, China, 100094)Li Guangbo; Cao Yazhong(Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China, 100094);The Effect of Nosema locustae Infection on Flying Ability of Locust (Locusta migratoria manilensis)[J];Acta Agrestia Sinica;1995-04
10 Zhang Yankun;Liu Guozhong(Institute of Hygiene and Evironmental Medicine,Academy ofMilitary Medical Sciences, Tianjin, 300050 );The Preparation and Applications of Chitin,Chitosan and their Derivatives[J];CHINA SURFACTANT DETERGENT & COSMETICS;1998-04
China Proceedings of conference Full-text Database 1 Hits
1 ZHOU Qiang, WANG Shu-Mao, WANG De-Cheng (China Agricultural Universi ty, Beijing, 100083);Problems on trapping control of locust pests as resources and the industrializing application[A];[C];2005
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 CAO Cheng-quan et al(College of Chemistry and Life Science,Leshan Teachers College,Leshan,Sichuan 614004);Problems and Countermeasures for the Resource Utilization of Locust[J];Journal of Anhui Agricultural Sciences;2008-35
2 CAO Cheng-quan et al(School of Chemistry and Life Sciences,Leshan Teachers College,Leshan,Sichuan 614004);Effect of the Artificial Feed with Different Content of Water on the Growth and Development of Locusta migratoria manilensis(Megen)[J];Journal of Anhui Agricultural Sciences;2008-36
3 ZHANG Gan-wei,LING Wen-hui,ZHONG Yu-lin,SUN Su-ling,XU Yan-xia(College of Life Science and Engineering,Huanggang Nomal College,huanggang 438000,China);Study on the Protein Extraction of Locusta migratoria manilensis with Aqueous Alkali[J];Hubei Agricultural Sciences;2009-01
4 HAN Ai-liang YE Qing-fu (Institute of Nuclear-Agricultural Sciences,Key Laboratory of Nuclear-Agricultural Science,Ministry of Agriculture,Zhejiang University,Hangzhou,Zhejiang 310029);PROBLEMS AND SUGGESTION ON RADIOISOTOPE TRACING EXPERIMENT[J];Journal of Nuclear Agricultural Sciences;2007-06
5 ZHANG Quan~1 HUANG Jian-zhong~1 YANG Zheng-min~2 ZHU Qi-song~1 YE Qing-fu~1 L Long~2 XU Bu-jin~1 CHEN Zi-yuan~1(1.Institute of Nuclear-Agricultural Sciences/Key Laboratory of Nuclear Agricultural Sciences of Ministry of Agriculture and Zhejiang Province,Zhejiang University,Hangzhou,Zhejiang 310029;2.Shanghai Institute of Organic Chemistry,Chinese Academy of Sciences,Shanghai 200032);PRELIMINARY STUDY ON METABOLISM OF PYRIBAMBENZ-PROPYL BY WHEAT MICROSOMES[J];Journal of Nuclear Agricultural Sciences;2008-01
6 YANG Zheng-min1,3 JI Zhi-qin1 Zhang Ji-wen1 Hu Zhao-nong1 YE Qing-fu2 WU Wen-jun1(1.Institute of Pesticide,Northwest University of Agriculture and Forestry,Yangling,Shaanxi 712100;2.Institute of Nuclear-Agricultural Science,Zhejiang University,Hangzhou,Zhejiang 310029;3.Shanghai Institute of Organic Chemistry,Chinese Academy of Sciences,Shanghai 200032);MICROSYNTHESIS OF INSECTICIDAL COMPONENT 6β-PROPOXYCELANGULIN V[J];Journal of Nuclear Agricultural Sciences;2008-02
7 XI Xiao-minga,ZHANG Hong-boa,WANG Ze-longb,HE Yanlanb(National University of Defense Technology a.College of Opto-electronic Science and Engineering;b.College of Science,Changsha 410073,Hunan,China);A Study on Biological Effect of Ultrasonic Irradiation on Eggs of Locust[J];Acta Laser Biology Sinica;2008-02
8 CAO Cheng-quan1,NIE Shu-xia2,CHEN Shen-zhi1,YE Bao-hua1,LIU Yu-sheng1(1.Shandong Agricultural University,College of Plant Protection,Tai′an 271000,China;2.Farming Exploitation Center of Center Borough,Jining,Shandong Province,Jinjing 272100,China);The Recyclical Exploitation and Use of Grasshopper[J];Journal of Economic Animal;2007-02
9 NING Chang-Ming1,2,WU Kong-Ming2,GAO Xi-Wu1,CHENG Hong-Mei3 (1. Department of Entomology,China Agricultural University,Beijing 100193,China; 2. State Key Laboratory for Biology of Plant Disease and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China; 3. Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China);Cloning of cDNA-encoding juvenile hormone epoxide hydrolase in Helicoverpa armigera(Hbner) and its expression in E. coli[J];Journal of Environmental Entomology;2009-02
10 NIU Cheng-Wei~ 1,2 , ZHANG Qing-Wen~1, YE Zhi-Hua~ 1,3 , LUO Li-Zhi~ 2, (1. College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China; 2. Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China; 3. Science & Technology Management Department, Chinese Academy of Agricultural Sciences, Beijing 100081, China);Analysis of genetic diversity in different geographic populations of the beet armyworm Spodoptera exigua (Lepidoptera:Noctuidae) with AFLP technique[J];Acta Entomologica Sinica;2006-05
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved