Genetic differentiation in five geographic populations of the oriental fruit fly,Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) in Yunnan Province
SHI Wei, YE Hui~*(Department of Biology, College of Life Sciences, Yunnan University, Kunming 650091, China)
Partial mitochondrial cytochrome oxidaseⅠ (COⅠ) region was sequenced from 27 individuals of 5 geographic populations of Bactrocera dorsalis (Hendel) from Ruili, Jinghong, Yuanjiang, Huanian and Hekou of Yunnan Province, respectively. Transition was higher than transversion, without insertion and deletion. In total, 23 haplotypes were identified within the sequences, with 27 sites showing polymorphism and 2 haplotypes shared. The Fst values among 5 populations were 0.0364-0.1364 (P0.05) and Nm 3.88-13.25. Phylogenetic trees of haplotypes were constructed by N-J method. Haplotypes did not accord with their geography. All results showed that genetic differences existed among 5 populations, but the degree was lower. The main reason of the genetic differentiation among 5 populations should be related to genetic isolation, development history and ecology adaptation of Bactrocera dorsalis.