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《Acta Entomologica Sinica》 2005-02
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Expression of the antifreeze protein gene of a pyrochroid beetle Dendroides canadensis in prokaryote and detection of the protein biological activity

LIU Zhong-Yuan, ZHANG Fu-Chun~(*), WANG Yun, LU Guo-Dong (Key Laboratory of Molecular Biology, College of Life Science and Technology, Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, Xinjiang University, Urumqi 830046, China)  
In order to express a pyrochroid beetle Dendroides canadensis antifreeze protein in E. coli BL21 and to investigate the properties of recombinant antifreeze protein, the D. canadensis afp gene was synthesized, cloned into the pGEX-4T-1, and transformed into E. coli BL21. The optimal inductive condition was selected by different experimental approaches. The results of SDS-PAGE analysis indicated that the antifreeze protein was expressed successfully in E.coli BL21. The expressed antifreeze protein, existing in the soluble and fusion form, with the molecular weight of about 40 kD, could increase low temperature resistance of bacteria as shown by the biological activity detection.
【Fund】: 国家科技攻关项目 (2001BA901A32)
【CateGory Index】: Q966
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