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《Chinese Journal of Applied Entomology》 2013-05
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Changes in susceptibility to chlorantraniliprole and detoxification enzymes of Plutella xylostella feeding on different host plants

YIN Fei;CHEN Huan-Yu;LI Zhen-Yu;LIN Qing-Sheng;HU Zhen-Di;ZHANG De-Yong;FENG Xia;Institute of Plant Protection,Guangdong Academy of Agricultural Sciences;Guangdong Provincial Key Laboratory of High Technology for Plant Protection;  
With the rapid development of high-throughput RNA sequencing( RNA-Seq) technology and the rapidly decreasing cost of this method,RNA-Seq is becoming an important tool for biological research,especially investigating gene function at the transcriptome level.RNA-Seq typically reads sequences rapidly with a certain percentage of sequence errors and bias producing a huge amount of data.RNA-Seq data analysis faces lots of challenges.Here,we describe different RNA sequencing platforms,raw data generation processes and data filtering and introduce short sequence alignment,transcriptome assembly,functional annotation and gene expression analysis.Finally,we briefly review the application of RNA-Seq in insects.The prospects of RNA-Seq techniques and their application are also discussed.
【Fund】: 公益性行业(农业)科研专项(201103021);; 国家支撑项目(2012BAD19B0);; 浙江省省部共建国家重点实验室培育基地开放基金项目(2010DS700124-KF1207);; 广州市农业攻关(GZCQC1202FG04002);; 广东省自然科学基金项目(10451064001006248和10151064001000025);; 广东省农业科学院院长基金(201012);; 促进科技服务业发展计划(2011B040100005)
【CateGory Index】: S436.3
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