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《Chinese Journal of Applied Entomology》 2015-02
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Establishment of a microinjection based RNA interference technique for Bemisia tabaci(Hemiptera: Aleyrodidae)

LI Ru-Mei;WANG Shao-Li;ZHANG You-Jun;Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences;  
RNAi is a powerful tool for revealing gene function. Microinjection is the most widely-used method to obtain systematic interference by injecting dsRNA into insects. However, it's very difficult to inject small insects, especially the sweetpotato whitefly, Bemisia tabaci. In order to verify the large-scale gene function of B. tabaci, it's necessary to develop an RNAi technique. We established a method to silence the B. tabaci CYP6CM1 gene, the effect of which was detected by injecting the 4th instar larvae of whiteflies with different dsRNA concentrations. The results show that injection with different concentrations of dsRNA had various silencing effects on the CYP6CM1 gene, with a maximum silencing efficiency of more than 80%. This successful RNAi technology successfully can be used on other whiteflies.
【Fund】: 国家863计划(2012AA101502);; 国家自然科学基金(31171857);; 北京室重点实验室2012年阶梯计划项目(Z121106002812038);; 蔬菜有害生物控制与优质栽培北京市重点实验室
【CateGory Index】: S433
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