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《Chinese Journal of Applied Entomology》 2015-02
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Establishment of a microinjection based RNA interference technique for Bemisia tabaci(Hemiptera: Aleyrodidae)

LI Ru-Mei;WANG Shao-Li;ZHANG You-Jun;Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences;  
RNAi is a powerful tool for revealing gene function. Microinjection is the most widely-used method to obtain systematic interference by injecting dsRNA into insects. However, it's very difficult to inject small insects, especially the sweetpotato whitefly, Bemisia tabaci. In order to verify the large-scale gene function of B. tabaci, it's necessary to develop an RNAi technique. We established a method to silence the B. tabaci CYP6CM1 gene, the effect of which was detected by injecting the 4th instar larvae of whiteflies with different dsRNA concentrations. The results show that injection with different concentrations of dsRNA had various silencing effects on the CYP6CM1 gene, with a maximum silencing efficiency of more than 80%. This successful RNAi technology successfully can be used on other whiteflies.
【Fund】: 国家863计划(2012AA101502);; 国家自然科学基金(31171857);; 北京室重点实验室2012年阶梯计划项目(Z121106002812038);; 蔬菜有害生物控制与优质栽培北京市重点实验室
【CateGory Index】: S433
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【Co-citations】
Chinese Journal Full-text Database 10 Hits
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2 WANG Zhi-ying;College of Forestry,Northeast Forestry University;;Functional Analysis of Hsp40 Gene in Lymantria dispar Mediated RNAi Technology[J];Journal of Anhui Agricultural Sciences;2014-26
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4 WANG Hao,LAI Duo,XU Han-Hong (Key Laboratory of Natural Pesticide and Chemical Biology,Ministry of Education,South China Agricultural University,Guangzhou 510642,China);Application and Prospect of RNA Interference in Pest Control and the Screening of Pesticide[J];World Pesticides;2012-03
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7 Zhang Hong;Du Yaqiong;Li Fengliang;Zhou Zhou;Gu Shuyu;Cheng Luogen;School of Life Sciences,Nanjing Normal University;Institute of Plant Protection,Guizhou Academy of Agriculture Science;;Cloning and Analysis of Ubiqitin Genes of Plutella Xylostella (L.)[J];Journal of Nanjing Normal University(Natural Science Edition);2013-03
8 SHI Huai-Xing;ZENG Hong-Mei;QIU De-Wen;Key Laboratory of Integrated Pest Management in Crops,Ministry of Agriculture,Institute of Plant Protection,Chinese Academy of Agricultural Sciences;;Identification,cDNA cloning and sequence anlysis of a peptidoglycan-recognition-like protein Gm21 in Galleria mellonella[J];Chinese Journal of Applied Entomology;2013-01
9 ZHAO Jin-cheng;WU Tian-min;LIU Li-hua;WANG Yang;HE Lin;School of Life Sciences,East China Normal University;;EcR-RNAi and azadirachtin treatments induced the abnormal proleg development in Spodoptera litura.[J];Journal of East China Normal University(Natural Science);2014-01
10 ZHU Bin;LIU Xiao-Ming;DU Meng-Fang;YIN Xin-Ming;AN Shi-Heng;College of Plant Protection,Henan Agricultural University;;Progress in insect bursicon[J];Acta Entomologica Sinica;2013-12
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