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《Anti-Infection Pharmacy》 2009-01
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Studies on Fluorescence Investigation of the Interaction of Artemisinin with Peroxidase

ZHANG Ben,ZOU Ting,XIE Hong-pin,WANG Jian-wen School of Pharmacy,Soochow University,Jiangsu Suzhou 215123,China  
Objective:To explore the interaction between artemisinin and peroxidase(horseradish peroxidase,HRP) and establish quantification method for determination of artemisinin in Chinese medicinal herb Artemisia annua L.Methods:Pyronine B was used as an indicator to study on the interaction between artemisinin and HRP by fluorescence detection.Results:The Michaelis-Menten parameters of the reaction including Km,vmax and Kcat were 2.72× 10-5 mol/L,1.74×10-4 mol/(L·s),24.27 s-1,respectively.The action sites were endperoxide moiety of artemisinin and the central metal ion of enzyme.Under optimal conditions,fluorescence intensity change of pyronine B was proportional to the artemisinin concentration.The linear regression equation was c = 0.168 4 ?F-0.814 3(the concentration measurement unit of artemisinin is 1.0×10-7mol/L) and the detection limit(3 σ) was 3.58× 10-8 mol/L.Conclusion:The interaction between HRP and artemisinin was in an enzyme substrate model.This method could be applied to determine the concentration of artemisinin in A.annua.
【Fund】: 国家自然科学基金项目(No.30772731);; 江苏省自然科学基金项目(No.BK2007051)
【CateGory Index】: R285.5
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Chinese Journal Full-text Database 4 Hits
1 CHEN Yang (National Laboratory of Molecular and Biomolecular Electronics,Southeast University,Nanjing,210093) ZHU Shimin\ CHEN Hongyuan (Department of Chemistry,Nanjing University,Nanjing,210096);Study on the Electrochemical Behaviors of Artemisinin(Qinghaosu) and Its Derivatives Ⅱ Reduction Mechanism of Artemisinin in the Presence of Hemin[J];Acta Scicentiarum Naturalum Universitis Pekinesis;2001-02
2 CHEN Li-hua, HUANG Fei, LIU Liu-zhan, SHEN Han-xi 1.Chemical School, Nankai University, Tianjin 300071, China 2.Chemical Department, Jishou University, Jishou 416000, China;Horseradish-peroxidase Catalytic Fluorescence Reaction with Pyronine B as Substrate for Determination of Glucose[J];Spectroscopy and Spectral Analysis;2003-05
3 LIN Jing-yu~(1), XIE Li-ping~(2) (1.School of Biotechnology, Jimei University, Xiamen 361021, China; 2.Department of Biological Science and Biotechnology, Tsinghua University, Beijing 100084, China) ;Effects of EDTA on the Activity of Alkaline Phosphatase from Pincata fucata[J];Journal of Jimei University Natural Science;2004-04
4 LU Shi ying, LI Hua zhong, CHEN Jian (The Key Laboratory of Industrial Biotechnology under Ministry of Education, Southern Yangtze University, Wuxi 214036, China);Effect of Ethylenediamine Tetraacetic Acid on Dye-decolorization by Lignin Peroxidase[J];Journal of Wuxi University of Light Industry;2002-04
Chinese Journal Full-text Database 10 Hits
1 GONG Yan et al(Jiangsu Suwei Microbiology Research Co.Ltd,Wuxi,Jiangsu 214063);Research on the Stability of Enzyme-labelled Antigen in AFB_1 ELISA Kit[J];Journal of Anhui Agricultural Sciences;2009-09
2 GAN Ning~(1,2),GE Cong-xin~11.Chemistry Department of Faculty of Science,Ningbo University,Ningbo Zhijiang 315211,China;2.Biochemistry Department of Faculty of Life Science,Nanjing University,Nanjing 210008,China;Reagentless Hydrogen Peroxide Biosensor Based on the Coimmobilization of Safranine T and Horseradish Peroxidase by Their Cross-Linking Effect[J];Journal of Transcluction Technology;2005-04
3 HUANG Lianxi,HE Yanlin,YANG Peihui*(Department of Chemistry,Jinan University,Guangzhou 510632,Guangdong,China);Breakage of the Peroxy Bond in Dihydroartemisinin Induced by Electrocatalysis of Copper(Ⅱ)[J];Chinese Journal of Catalysis;2008-03
4 CHEN Peice,ZHANG Zhaohui,XIE Xuefeng(College of Biotechnology and Environmental Engineering,Zhejiang University of Technology,Hangzhou 310014);Research Advances in Co-immobilization of Horseradish Peroxidase[J];Materials Review;2010-09
5 DONG Liang, HUANG Min-sheng, XIE Bin, WANG Zhong-hua, MA Li-hua,LIANG Huan (Department of Environmental Science, East China Normal University, Shanghai 200062);Advances in Research of Enzymology and Molecular Biology Pertaining to White Rot Fungi[J];Environmental Science and Technology;2005-05
6 Song Gui-Lan1,Ren Hao1,Jia Su-Zhen1,Gu Yan-Chun2,Tian Yong-Fu11(School of Chemistry and Chemical Engineering,University of Ji'nan,Ji'nan 250022)2(The Hospital of University of Ji'nan,Ji'nan 250022);Spectrophotometric Determination of Protein Based on the Enzymatic Catalytic Reaction[J];Chinese Journal of Analytical Chemistry;2007-05
7 LV Sheng-hua1,LIU Gang1,MA Yan-fen1,HU Jing1,ZHANG Guo-yun2(1.College of Resource and Environment,Shaanxi University of Science & Technology,Xi'an 710021,China;2.School of Chemistry and Chemical Engineering,Shaanxi University of Science & Technology,Xi'an 710021,China);Study on preparation and properties of copolymer of p-hydroxybenzoic acid and sulfanilic acid with HRP catalysis[J];Journal of Functional Materials;2010-08
8 CAO Zhan-wang1,2,WANG Da-peng1,GAN Xi1(1 Guangxi Institute of Fishery,Nanning 530021,China;2 Fishery Research Institute,Guangxi University,Nanning 464000,China);Advances in Pinctada martensii(Dunker)and its seawater pearl during recent years[J];Guangxi Agricultural Sciences;2009-12
9 Hong Wei-jie, Zhang Zhao-hui, Lu Guo-ying (College of Biological and Environmental Engineering, ZJUT, Hangzhou310014, China);The Research Advances on Immobilization of Horseradish Peroxidase[J];Modern Food Science and Technology;2006-01
10 TANG Ning-li ,XIA Hai-ming,MENG Xing-long,LING Yue-fei and TANG Jian-zhen(College of Chemistry and Bioengineering,Guilin University of Technology,Guilin 541004),Fenxi Shiyanshi,2011,30(9):85~88;Determination of hydrogen peroxide based on the oxidation reaction of phenol using a β-cyclodextrin-Iron(Ⅲ)-tetracarboxyphthalocyanine inclusion complex as mimetic peroxidase[J];Chinese Journal of Analysis Laboratory;2011-09
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Guo Zhongxian; Shen Hanxi; Li Li (Department of Chemistry, Nankai University, Tianjin 300071);Mimetic Peroxidase-Catalyzed Oxidation of Phenylfluorone and Its Analytical Applications[J];Chinese Journal of Analytieal Chemistry;1998-12
2 Zhu Min, Han Shubo, Yuan Zhuobin ( Department of Chemistry, Graduate School, Universit of Science & Technology of China, Beijing 100039)Shen Hanxi (Department of Chemistry, Nankai University, Tianjin 300071);Nitrosulfonate S as the Substrate for Horseradish Peroxidase in Enzyme-Linked Immunoassay[J];Chinese Journal of Analytical Chemistry;1999-04
3 Yuanzong Li;Jun Gao;Yunxiang Ci(Depertment of Chemistry,Peking University,Beijing,100871);Glucose Dtermination Based on a New Fluorescent Reaction Catalyzed by Hemin[J];JOURNAL OF ANALYTICAL SCIENCE;1995-03
4 Ci Yunxiang, Chen Lie, Wei Shan (Department of Chemistry, Peking University, Beijing);Peroxidase Catalytic Fluorescence Reaction with Tyrosine as Substrate[J];Chemical Research In Chinese Universities;1990-01
5 ZHAO Kai-Cun, CHEN Zhi-Xi~*, LIN Bing-Liu~*, GUO Xing-Bo~*, Li Guo-Qiao~*, SONG Zhen-Yu(Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing) (Guangzhou College of Traditional Chinese Medicine) ~*;STUDIES ON THE PHASE I CLINICAL PHARMACOKINETICS OF ARTESUNATE AND ARTEMETHER~+[J];The Chinese Journal of Clinical Pharmacology;1988-02
6 LI Hua zhong,ZHANG Yan fang (School of Bioengineering,Wuxi University of Light Industry,Wuxi 214036,China);White rot fungi and the treatment of dye-containing wastewater[J];Industrial Water Treatment;2001-05
7 LI Hua-zhong, ZHANG Yan-fang, HUA Zhao-zhe, CHEN Jian (The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Southern Yangtze University, Wuxi, Jiangsu 214036, China);Selective Synthesis of Lignin Peroxidase and Manganese Peroxidases by Phanerochaete chrysosporium[J];The Chinese Journal of Process Engineering;2002-02
8 LI Huirong\ LI Huabing\ LI Wenqing\ ZHAO Fei\ GENG Lei (Department of Energy Engineering, Jiangsu Institute of Petrochemical Technology, Changzhou 213016);Decolorization and Biodegradation of Six Kinds of Dyes by the White Rot Fungus Phanerochaete chrysosporium[J];RESEARCH OF ENVIRONMENTAL SCIENCES;1999-03
9 Zou Chongda(Dept. of Chem. & Biochem. Eng., Huaqiao Univ., 362011, Quanzhou);Fast Absorption of Artemether by Rat and ItsDistribution in the Body of Rat[J];JOURNAL OF HUAQIAO UNIVERSITY(NATURAL SCIENCE);1996-03
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