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《Anti-Infection Pharmacy》 2009-01
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Studies on Fluorescence Investigation of the Interaction of Artemisinin with Peroxidase

ZHANG Ben,ZOU Ting,XIE Hong-pin,WANG Jian-wen School of Pharmacy,Soochow University,Jiangsu Suzhou 215123,China  
Objective:To explore the interaction between artemisinin and peroxidase(horseradish peroxidase,HRP) and establish quantification method for determination of artemisinin in Chinese medicinal herb Artemisia annua L.Methods:Pyronine B was used as an indicator to study on the interaction between artemisinin and HRP by fluorescence detection.Results:The Michaelis-Menten parameters of the reaction including Km,vmax and Kcat were 2.72× 10-5 mol/L,1.74×10-4 mol/(L·s),24.27 s-1,respectively.The action sites were endperoxide moiety of artemisinin and the central metal ion of enzyme.Under optimal conditions,fluorescence intensity change of pyronine B was proportional to the artemisinin concentration.The linear regression equation was c = 0.168 4 ?F-0.814 3(the concentration measurement unit of artemisinin is 1.0×10-7mol/L) and the detection limit(3 σ) was 3.58× 10-8 mol/L.Conclusion:The interaction between HRP and artemisinin was in an enzyme substrate model.This method could be applied to determine the concentration of artemisinin in A.annua.
【Fund】: 国家自然科学基金项目(No.30772731);; 江苏省自然科学基金项目(No.BK2007051)
【CateGory Index】: R285.5
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