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《Science & Technology Review》 2009-16
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Selection and Community Structure Analysis of Two New Syntrophic Acetogen Cocultures

LI Jianzheng, SUN Qian, LIU Feng, GAO Chenchen State Key Laboratory of Urban Water Resource and Environment;School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China  
Among the bacteria colony in the anaerobic digestion system, the Hydrogen-Producing Acetogens(HPAs) are in between acidogenic fermentation bacteria and those with methanogens in the functional niche.They can digest or translate volatile fatty acids and ethanol, produced by acidogenic fermentation bacteria, into acetic acid and CO2/H2 which can be directly derived by methanogens while methane is being produced.HPAs are quite difficult to be isolated by pure and ampliative cultures due to their accrete characteristics.In order to study HPA's physiological and ecological characteristics and to establish the scientific basis for the development of highly efficient anaerobic biological treatment processes, the syntrophic acetogen cocultures are enriched and selectively bred from the anaerobic activated sludge with methanogens by selective medium, using propionic and butyric acids as the only carbon source.Furthermore, the community structure of the selected syntrophic acetogen cocultures are analyzed by Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis(PCR-DGGE).With the medium and the culture condition being improved, two syntrophic acetogen cocultures, namely 7-m-2a and 11-O-1, are obtained.These two cocultures show a high ability in degrading propionic and butyric acids and producing acetic acid, with no CH4 or H2S evolved in both culture systems.The analysis results of PCR-DGGE indicate that specialized syntrophic acetogen bacteria Desulfotomaculum sp.Iso-W2 and its associated bacterias are dominated in the syntrophic acetogen cocultures.The associated bacteria are uncultured bacteria 054E12_B_DI_P58 and Sedimentibacter sp.JN18_A14_H, which can use formate and H2/CO2.The two new syntrophic acetogen cocultures obtained can provide a sample for investigating the physiological characteristics of HPA in depth.
【Fund】: 国家高技术研究发展计划(863计划)项目(2006AA05Z109)
【CateGory Index】: X172
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