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Isolation of Three Novel Fermentative H2-Producing Bacteria and Comparison of Their H2-Producing Ability under Different Conditions

CHEN Ying1,2,REN Nanqi1,CHENG Yao1,LI Yongfeng1,ZHANG Dawei2(1.School of Municipal and Environmental Engineering,Harbin Institute of Technology,Harbin 150090,China;2.Department of Biology,Harbin Normal University,Harbin 150080,China)  
Hydrogen gas is perfect and clean energy.The study of biohydrogen producing techniques has been a hot spot today.Under anaerobic condition,many bacteria can ferment complex organic compound to generate hydrogen gas.In this paper,three novel fermentative hydrogen producing bacteria isolated from an active sludge sample were chose to compare and observe their morphology characteristics by methods of biochemistry and electronic microscope.Furthermore,the variations and differences of cumulative production of hydrogen gas,density of bacteria and end liquid products were studied under different pH conditions.Their maximum cumulative H2-producing quantities of unit volume substrate were achieved and the optimum of biohydrogen process by pure culture was suggested to offer references for intensifying biohydrogen process by mixed culture.
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