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《Bulletin of Science and Technology》 2008-03
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Separation and Determination of Organic Acids in Flower,Branch and Leaf Extract of Prunus mume by HPLC

PAN Huihui,WU Xiaoqin,LU Baiyi,ZHANG Ying(College of Biosystems Engineering and Food Science,Zhejiang University,Hangzhou 310029,China)  
A high-performance liquid chromatography(HPLC)method for the determination of organic acids in flower,branch and leaf extract of Prunus mume is proposed here.Agilent TC-C18 column(250 mm×4.6 mm i.d.,5μm) was used at 30℃ and mobile phase was 0.01mol/L KH2PO4 solution containing 3% methanol with pH2.85 at 1.0 mL /min flow speed,detection wavelength was at 210 nm.The relative standard deviations were 0.36%~1.61% and the recoveries were 92.7%~104.2%.The correlation coefficients for the linear equations were 0.9943~1.0000.The method was rapid and accurate for quantitative analysis of ten organic acids including oxalic,tartaric,malic,L-ascorbic,lactic,acetic,citric,maleic,fumaric,succinic acids.Studies have showed that the total amount of organic acids in branch extract was 5.75% and they were mainly were citric acid and tartaric acid,and in leaf extract was 5.86% and mainly were oxalic acid and citric acid.Flower extract contained much less organic acids.
【CateGory Index】: S662.4
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