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《Bulletin of Science and Technology》 2009-06
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Studies on the Optimum Culture Conditions of Vibrio splendidus M-1

MA Aimin1,2,YAN Maocang1,XIE Qilang1,CHEN Shaobo1,SHAN Lezhou1,CHANG Weishan2(1.Zhejiang Mariculture Research Institute,Wen zhou 325005,China;2.Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource,Wenzhou 325005,China;3.Department of Animal Sciences and Technology,Shandong Agricultural University,Tai’an,271018,China)  
The effects of culture time,temperature,salinity,initial pH and initial concentration on the growth of strain M-1 were studied by gradient method;Compositions of Zobell 2216 E culture media were optimized by orthogonal design.The results showed that: the optimum time,temperature,salinity,initial pH and initial concentration were 48 h,32 ℃,40,8.0 and 1×105 CFU / mL respectively;In addition,the optimized elements of culture media are: Peptone 7.5 g / L,Yeast extract 1.5 g / L,CuSO4 0.075 mg / L and CaCl2 0.04 g / L.
【Fund】: 浙江省科技厅科研院所青年人才计划项目(2006R20001);; 浙江省科技厅科研院所专项公益技术攻关项目(2007F10009);; 温州市科技兴海项目(S20070051)联合资助
【CateGory Index】: S941
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【References】
Chinese Journal Full-text Database 2 Hits
1 TAO Jia-fa,LAI Ying-tiao,PAN Hou-jun,SHI Cun-bin,WU Shu-qin(Pearl River Fisheries Research Institute,Chinese Academy of Fisheries Sciences,Guangdong Key Laboratory of Aquatic Animal Immune Techniques,Guangzhou 510380,China);Optimization Culture of Vibrio harveyi EcGY020401[J];Biotechnology;2010-05
2 TAO Jia-fa,LAI Ying-tiao,REN Yan,KANG Guang-hui,ZHANG You,SHI Cun-bin,WU Shu-qin(PearlRiver Fisheries Research Institute,Chinese Academy of Fisheries Sciences,Guangdong Key Laboratory ofAquatic Animal Immune Techniques,Guangzhou 510380,China);Preliminary Study on Condition for Inactivation of Vibrio by Formalin[J];Chinese Journal of Biologicals;2011-01
【Citations】
Chinese Journal Full-text Database 6 Hits
1 MO Zhao Lan, MAO Yun Xiang , CHEN Shi Yong, ZHANG Zhen Dong XU Yong Li, ZHANG Pei Jun (Key Laboratory of Experimental Marine Biology, The Chinese Academy of Sciences; Institute of Oceanology, The Chinese Academy of Sciences, Qingdao, 266071) (Marine Life of Sciences, China Ocean University, Qingdao, 266003);IDENTIFICATION AND PHYLOGENETIC ANALYSIS OF ONE PATHOGENIC BACTERIUM ASSOCIATED WITH SWOLLEN ABDOMEN OF CULTURED FLOUNDER (PARALICHTHYS OLIVACEUS) LARVAE[J];Oceanologia Et Limnologia Sinica;2003-02
2 XIE Jian-jun1,2,WANG Yin-geng1,ZHANG Zheng1,RONG Xiao-jun1(1.Key Laboratory for Sustainable Utilization of Marine Fisheries Resources,Ministry of Agriculture,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China;2.Biology Science&Technology College,Shanghai Fisheries University,Shanghai 200090,China);Rapid detection of two pathogens of skin ulcer syndrome in cultured Apostichopus japonicus by Dot-ELISA[J];Marine Sciences;2007-08
3 PAN Xiao-yi1,SHEN Jin-yu1,YIN Wen-lin1,et al(1.Zhejiang Institute of Freshwater Fisheries, Huzhou 313001,China);Studies on the Optimal Culture Conditions of Vibrio harveyi BK-1 from Sparus macrocephalus[J];Journal of Zhejiang Ocean University;2005-03
4 ZHANG Chun-yun 1,2, WANG Yin-geng1, RONG Xiao-jun1 (1. Key Laboratory for Sustainable Utilization of Marine Fisheries Resources Certificated by the Ministry of Agriculture, Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China; 2.School of the Ocean,Harbin Institute of Technology,Weihai 264209,China);Isolation and identification of causative pathogen for skin ulcerative syndrome in Apostichopus japonicus[J];Journal of Fisheries of China;2006-01
5 LUO Zhu hua, HUANG Xiang ling, WANG Lin, PEI Yao wen, YE De zan (The Key Laboratory of State Oceanic Administration(SOA) for Marine Biotechnology/Third Institute of Oceanography, SOA, Xiamen 361005,China);Screening of marine bacteria with antimicrobial activity[J];Journal of Oceanography In Taiwan Strait;2002-02
6 WANG Yin-geng~(1),SUN Su-feng~(1,2),RONG Xiao-jun~(1)(1.Key Laboratory for Sustainable Utilization of Marine Fisheries Resources,Ministry of Agriculture,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China;2.Life Science and Technology College,Ocean University of China,Qingdao 266003,China);Stomach ulcer disease in auricularia of sea cucumber(Apostichopus japonicus) and its etiological identification[J];Journal of Fishery Sciences of China;2006-06
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LIU Li-hui et al(Pearl River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou,Guangdong 510380);Isolation and Classification of Pathogenic Bacterium Caused by Gill-rote Disease in Channel Catfish(Ictalunes punctatus)[J];Journal of Anhui Agricultural Sciences;2008-17
2 NIU Yu-feng et al(Key Laboratory of Mariculture,Ministry of Educaion,Ocean University of China,Qingdao,Shandong 266003);Comparative Study on Seasonal Variation of Heterotrophic Bacteria Flora Associated with Intestine of Sea Cucumber in Feeding and Unfeeding Ponds[J];Journal of Anhui Agricultural Sciences;2009-27
3 ZHANG An-guo(College of Animal Husbandry and Veterinary Medicine,Liaoning Medical University,Jinzhou,Liaoning 121001);Advance of the Pathological Researches on Apostichopus japonicus[J];Journal of Anhui Agricultural Sciences;2009-35
4 BAO Zeng-hai1,KONG De-ping2,WANG Zeng-chi3,MA Gui-zhen1,XIAO Yun1(1.College of Marine Sciences,Huaihai Institute of Technology,Lianyungang 222005,China;2.Agricultural and Forest Academy of Cangzhou,Cangzhou 061001,China;3.Cangzhou Vocational College of Technology,Cangzhou 061001,China);Isolation of Marine Bacteria Against Vibrio and Detection of Antimicrobial Activity[J];Fisheries Science;2009-01
5 ZHONG He1,LIU Xing2,3,GAO Xiang-gang3,LI Yun-feng3,HE Chong-bo3(1.Liaoning Entry-exit Inspection and Quarantine Bureau,Dalian 116001,China;2.College of Life Science and Technology,Dalian Fisheries University,Dalian 116023,China;3.Liaoning Ocean and Fisheries Science Research Institute,Liaoning Key Laboratory of Marine Fishery Molecular Biology,Liaoning Marine Environmental Monitoring General Station,Dalian 116023,China);16S rRNA Technique and Its Application in Aquaculture[J];Fisheries Science;2009-04
6 GUAN Xiao-yan,ZHOU Zun-chun,JIANG Bing,DONG Ying,WANG Xiao-yu,JIANG Bei,YANG Ai-fu,CHEN Zhong,WANG Bai,SUN Da-peng(Liaoning Ocean and Fisheries Science Research Institute,Liaoning Marine Environmental Monitoring General Station,Liaoning Key Lab of Marine Fishery Molecular Biology,Dalian 116023,China);Environmental Bacterial Diversity in Sea Cucumber (Apostichopus japonicus) Culture Under Different Salinities Based on DGGE Fingerprint[J];Fisheries Science;2011-05
7 ZHANG Chun-yun1,CHEN Guo-fu1,XU Zhong1,YAN Pei-sheng1,WANG Yin-geng2 ( 1.College of the Ocean,Harbin University of Technology,Weihai 264209,China;2.Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China);Advances in Siseases of Sea Cucumber Apostichopus japonicus——A review[J];Fisheries Science;2011-10
8 CHENG Peng-fei1,LIANG Shao-kang1,CHENG Guo-jun1, NONG Xu-hua1,2,QI Shu-hua2,XIONG Hai-rong1(1.College of Life Science,South Central University for Nationalities,Wuhan 430074,China;2.Key Laboratory of Tropical Marine Environmental Dynamics,Chinese Academy of Sciences,Guangzhou 510301,China);Identification and Culture Condition Optimization of Metabolites in Marine Gorgonian-symbiotic Bacillus sp.[J];Fisheries Science;2011-11
9 ZENG Hai-yan,XIE Xiao-bao,HUANG Xiao-mo,LI Cai-ling,OU-YANG You-sheng,CHEN Yi-ben(Key Laboratory of Microbial Culture Collection and Application,Detection Center of Microbiology, Guangdong Institute of Microbiology,Guangzhou 510070,China);Isolation and screening of marine antagonistic bacteria and identification of a strain of antagonistic Bacillus sp.[J];Journal of Marine Sciences;2011-04
10 MA Yue-xin,WANG Yan,LIU Lu,LIU Xin,ZHANG Ze-yuRC, Dalian Fisheries Univ., Dalian 116023, China);Antimicrobial activities of epiphytic bacteria on seaweeds in intertidal zone in Dalian coast[J];Journal of Dalian Fisheries University;2003-04
China Proceedings of conference Full-text Database 4 Hits
1 Huang Huawei~(1,2) Wang Yingeng~2 Chen Xia~3 Zhang Fengping~4 1 Fishery Sciences Institute of Hunan Province,Changsha,410153; 2 Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences, Qingdao,266071; 3 Sunny Oceans Aquaculture Technology Co.,Ltd,Qingdao 266071; 4 Fisheries Institute of Zhoushan,Zhoushan,316000;The Relation Between Skin Ulcer Syndrome Occurrence of Cultured Apostichopus Japonicus and Cultural Environment During Aestivation[A];[C];2011
2 Xue Delin~1,Hu Jiangchun~1,Wang Guoan~2,Liu Li~1,Wang Shujin~1 (1 Institute of Applied Ecology,Chinese Academy of Sciences,Shenyang,110016 2 Dalian Wudao Rare Seafood Technology Development Company,Dalian,116312);Effects of Rhodotorula mucilaginosa,Rhodopseudomonas sphaeroides,and Biochemical Fulvic Acid on the Seedling and Breeding of Sea Cucumber[A];[C];2008
3 Ma Guizhen~1 Kong Deping~2 Wang Zengchi~2 Bao Zenghai~1 Lu Mingsheng~1 1 College of Marine Sciences,HuaiHai Institute of Technology,Lianyunguang 222005; 2 Cangzhou Vocational college of Technology,Cangzhou 061001;Study on antimicrobial activity of marine bacteria against plant pathogenic fungus[A];[C];2008
4 Zeng Haiyan;Xie Xiaobao;Huang Xiaomo;Ouyang Yousheng;Chen Yiben;Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,Guangdong Detection Center of microbiology,Guangdong Institute of Microbiology;;Isolation of Marine Bacteria with Antimicrobial Activity[A];[C];2008
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 LI Yuan-Yuan CAO Hai-Peng DENG Lu YANG Xian-Le~(Aquatic Pathogen Collection Center of Ministry of Agriculture,Shanghai Fisheries University,Shanghai 200090,China);Isolation of Pathogenic Bacteria Aeromonas hydrophila from Acipenser baerii and Immune Effect of Its whole Cell Vaccine[J];Chinese Journal of Zoology;2008-06
2 MAO Zhi-juan~1, LOU Dan~1,WU Jian-jun~2 (1.Ningbo Fisheries Research Institute, Ningbo 315012,China;2. Huazhong Agricultural University, Wuhang 430070,China);Studies on Culture Technological Conditions of Vibrio harveyi Y91301[J];Fisheries Science;2004-03
3 SUN Yi-min1,2,LI Nan1,2,SONG Jie2,LI Xiao-yue2,SHEN Hong-qi3,WANG Feng-min3,LI Quan-zhen3,CAO Jie-ying3,ZHAO Bao-hua2 1.Hebei Chemical Engineering and Pharmaceutical College,Shijiazhuang 050026,China;2.College of Life Science,Hebei Normal University,Shijiazhuang 050016,China;3.Department of Diseases Supervision,Hebei Fisheries Bureau,Shijiazhuang 050011,China;Isolation and Identification of Bacterial Pathogen Aeromonas hydrophila and Development of the Inactivated Vaccine in Japanese Flounder Paralichthys olivaceus Diseased with Ascites[J];Fisheries Science;2009-11
4 PAN Xiao-yi,SHEN Jin-yu,YIN Wen-lin,CHAO Zheng (Key Laboratory of Fish Health and Immunology,Chinese Academy of Fishery Science,Zhejiang Institute of Freshwater Fisheries,Huzhou 313001,China);Vibriosis and their mechanisms in aquatic animals[J];Journal of Dalian Fisheries University;2006-03
5 DONG Chuan-fu,LIN Tian-long,GONG Hui, YU Fu-song,YANG Jin-xian (Animal Husbandry and Veterinary Medicine Institute, Fujian Academy of Agricultural Sciences, Fuzhou,Fujian,350013,China);Analysis of the Partial Bio-Activities,Component and Major Protein Pattern of the Extracellular Products(ECPs) Produced by Aeromonas hydrophila[J];Progress In Veterinary Medicine;2005-05
6 Ma Yue, Zhang Yuanxing, Sun Xiuqin  (State key Lab of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237) ( First Institute of Oceanography, State Oceanic Administration, Qingdao 266003);Production of Marine Fish Vaccine for Vibriosis from Vibrio anguillarum: Optimization of New Culture Medium for High density Culture[J];High Technology Letters;2001-07
7 DONG Xue, ZHONG Qing-pin, HUANG An-cheng, LEI Hong-tao, SUN Yuan-ming (College of food science, South China Agriculture University, Food Quality and Safety Key Lab of Guangdong Province, Guangzhou 510642);Development of a Direct Competitive ELISA Method for Tetrodotoxin Detection[J];Modern Food Science and Technology;2009-08
8 QIAN Yunxia WANG Guoliang (Fisheries Department,Ningbo University) SHAO Jianzhong* (College of Life Science, Zhejiang University*);A REVIEW OF PRINCIPAL BACTERIAL DISEASES OF MARICULTURE FISH[J];Transaction of Oceanology and Limnology;2001-02
9 WU Hou-Bo, PAN Jin-Pei (South China Sea Institute of Oceanology, The Chinese Academy of Sciences, Guangzhou, 510301);PURIFICATION AND CHARACTERIZATION OF THE EXOTOXIN Vm-Pm PRODUCED BY VIBRIO MIMICUS[J];Oceanologia Et Limnologia Sinica;2002-01
10 MO Zhao Lan, MAO Yun Xiang , CHEN Shi Yong, ZHANG Zhen Dong XU Yong Li, ZHANG Pei Jun (Key Laboratory of Experimental Marine Biology, The Chinese Academy of Sciences; Institute of Oceanology, The Chinese Academy of Sciences, Qingdao, 266071) (Marine Life of Sciences, China Ocean University, Qingdao, 266003);IDENTIFICATION AND PHYLOGENETIC ANALYSIS OF ONE PATHOGENIC BACTERIUM ASSOCIATED WITH SWOLLEN ABDOMEN OF CULTURED FLOUNDER (PARALICHTHYS OLIVACEUS) LARVAE[J];Oceanologia Et Limnologia Sinica;2003-02
【Secondary References】
Chinese Journal Full-text Database 3 Hits
1 TAO Shi,HE Fang-fang,LIU Xue-zhu,HE Jian-yu,WANG Jian-xin(Laboratory for Marine Living Resources and Molecular Engineering,Zhejiang Ocean University,Zhoushan 316000,China);Research Progress of Pathogenic Microorganisms in Fish in Mariculture[J];Fisheries Science;2013-03
2 CHEN Si-Qing;LI Jie;HAN Yin;TANG Lei;BAI Hai-Meng;LIU Chang-Lin;ZOU An-Ge;QI Zi-Zhong;Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences;Lab of Marine Microbiology,College of Marine Life Sciences,Ocean University of China;Shandong Anyuan Fishery Limited Corporation;;Rapid Selection and Protective Effect of Potential Probiotics Against Vibrio splendidus from Mud of Sea Cucumber Culture Area[J];Periodical of Ocean University of China;2014-11
3 WU Shuqin;TAO Jiafa;GONG Hua;CHANG Ouqin;REN Yan;LIU Lihui;CHEN Zonghui;LAI Yingtiao;Key Laboratory of Fishery Drug Development,Ministry of Agriculture; Key Laboratory of Aquatic Animal Immune Technology,Pearl River Fishery Research Institute,Chinese Academy of Fishery Sciences;;Situation and tendency of the fishery vaccine development[J];Chinese Fishery Quality and Standards;2014-01
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 MAO Zhi juan 1,LIU Guo yong 2,CHEN Chang fu 2 (1 Ningbo Fishery Research Institute,Ningbo 315012; 2 Key Laboratory of Agricultural Microbiology, Ministry of Agriculture);Isolation and Identification of Pathogenic Bacteria Causing Ulcerosis in Large Yellow Croaker (Pseudosciaena crocea)[J];Journal of Anhui Agricultural University;2002-02
2 DENG Huan,SUI Xi-lin (Liaoning Marine Fisheries Research Institute, Liaoning Open Lab of Applied Marine Biology,Dalian 116023,China);Common Epidemic of Apostichopus japonicus in Incubation Period[J];Fisheries Science;2004-03
3 ZANG Hong-mei~(1,2),FAN Jing-feng~2,WANG Bin~1(1.School of Life Science and Technology,Dalian Fisheries Univ.,Dalian 116023,China;2.National Marine Environmental Monitoring Center,Dalian 116023,China);Detection of Vibrio parahaemolyticus by Dot-ELISA[J];Journal of Dalian Fisheries University;2006-01
4 Mo Zhaolan, Mao Yunxiang , Chen Shiyong, Zhang Zhendong, Zhang Peijun (Experimental Marine Biology Laboratory, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071) ( College of Marine Life Sciences, Ocean University of Qingdao,;Classification and Molecular Biological Analysis of One Pathogenic Bacterium Associated with Hamorrhages of Cultured Flounder[J];High Technology Letters;2001-12
5 Sun Yi Chen Dou(Institute of Oceanology, Academia Sinica, Qingdao);THE MICROBIAL COMPOSITION OF STICHOPUS JAPONIC-US AND ITS PHYSIOLOGICAL PROPERTY[J];Oceanologia Et Limnologia Sinica;1989-04
6 YOU Feng; WANG Ke-ling, XIANG Jian-hai, XU Cheng, WU Su-qi(Institute of Oceanology, ne Chinese Acadeny of Sciences, Qingdao, 266071);BIOCHEMICAL GENETIC ANALYSIS OF ISOZYMES ON THE LEFT-EYED FLOUNDER, PARALICHTHYS OLIVACEUS (T.ET S.) IN THE COASTAL WATERS OF SHANDONG[J];OCEANOLOGIA ET LIMNOLOGIA SINICA;1999-02
7 ZHU Xin-hua; WANG Yun-feng; LIU Dong(Institute of Oceanology, The Chinese Academy of Sciences,Qingdao, 266071);EFFECTS OF TEMPERATURE ON THE ECOLOGICAL PATTERNS OF RESOURCES RECRUITMENT OF BASTARD HALIBUT, PARALICHTHYS OLIVACEUS (T. ET S.)[J];OCEANOLOGIA ET LIMNOLOGIA SINICA;1999-05
8 WANG Hong Tian, ZHANG Pei Jun (Experimental Marine Biology Laboratory, Institute of Oceanology, The Chinese Academy of Sciences, Qingdao, 266071);ACTIVITIES OF DIGESTIVE ENZYME IN DIFFERENT TISSUES OF PARALICHTHYS OLIVACEUS[J];Oceanologia Et Limnologia Sinica;2002-05
9 MO Zhao Lan, MAO Yun Xiang , CHEN Shi Yong, ZHANG Zhen Dong XU Yong Li, ZHANG Pei Jun (Key Laboratory of Experimental Marine Biology, The Chinese Academy of Sciences; Institute of Oceanology, The Chinese Academy of Sciences, Qingdao, 266071) (Marine Life of Sciences, China Ocean University, Qingdao, 266003);IDENTIFICATION AND PHYLOGENETIC ANALYSIS OF ONE PATHOGENIC BACTERIUM ASSOCIATED WITH SWOLLEN ABDOMEN OF CULTURED FLOUNDER (PARALICHTHYS OLIVACEUS) LARVAE[J];Oceanologia Et Limnologia Sinica;2003-02
10 SONG Li-chao~(1,2), FAN Jing-feng~1, LIU Shu-xi~1,LIANG YU-bo~1 (1.National Marine Environmental Monitoring Center,Dalian 116023,Chian; 2. Dalian Fisheries University,Dalian 116023,Chian);Research progress on fast detection of pathogenic marine vibrio[J];Marine Environmental Science;2005-01
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