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Studies on the Optimum Culture Conditions of Vibrio splendidus M-1

MA Aimin1,2,YAN Maocang1,XIE Qilang1,CHEN Shaobo1,SHAN Lezhou1,CHANG Weishan2(1.Zhejiang Mariculture Research Institute,Wen zhou 325005,China;2.Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource,Wenzhou 325005,China;3.Department of Animal Sciences and Technology,Shandong Agricultural University,Tai’an,271018,China)  
The effects of culture time,temperature,salinity,initial pH and initial concentration on the growth of strain M-1 were studied by gradient method;Compositions of Zobell 2216 E culture media were optimized by orthogonal design.The results showed that: the optimum time,temperature,salinity,initial pH and initial concentration were 48 h,32 ℃,40,8.0 and 1×105 CFU / mL respectively;In addition,the optimized elements of culture media are: Peptone 7.5 g / L,Yeast extract 1.5 g / L,CuSO4 0.075 mg / L and CaCl2 0.04 g / L.
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