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《Bulletin of Science and Technology》 2014-03
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Solvent Effect on The Extraction of Cholesterol from Egg-yolk

Xu Biao;Chen Meilan;Yu Yourong;Hhuang Xianghong;College of Biology and Environmental Engineering,Zhejiang Shuren University;  
Cholesterol was extracted by high performance liquid chromatography with RP-C18 column(4.6 mm × 150 mm, 5 μL) and the mobile phase was 100% methanol; flow rate: 1 mL/min, column temperature: 25 ℃; detection wavelength: 205 nm; injection volume: 10 μL. The pre-treatment for the extraction of yolk cholesterol were conducted under different extraction solvent, such as cyclohexane, petroleum ether, ethyl ether- free, acetone, then detected its content and analyzed the extraction efficiency. It was found that the optimum extraction solvent was petroleum ether: acetone = 6:1(V/V), the egg yolk cholesterol content extracted was 13.94 mg/g.
【Fund】: 大学生科研创新学生资助项目(2012C1019)
【CateGory Index】: S879.3
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