Determination of Tryptophan in the Feedstuffs by HPLC
LI Jia sheng, CHEN Zhong ming (Inst. of Feed Sci.,Zhejiang University,Hangzhou 310029,China)
Feedstuffs were hydrolysed at 110℃ for 24 h with Ba(OH) 2 solution and a little of starch,the products were adjusted to pH 5-6 with 4 mol/L HCl.Tryptophans were determinated by High Performance Liquid Chromatography with pH 4.5 NaAc buffer and 15% methanol as mobile phase. This method is simple, rapid and accurate. Its recovery is between 97.7%-104.1%.