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《BULLETIN OF SCIENCE AND TECHNOLOGY》 1999-03
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Determination of Tryptophan in the Feedstuffs by HPLC

LI Jia sheng, CHEN Zhong ming (Inst. of Feed Sci.,Zhejiang University,Hangzhou 310029,China)  
Feedstuffs were hydrolysed at 110℃ for 24 h with Ba(OH) 2 solution and a little of starch,the products were adjusted to pH 5-6 with 4 mol/L HCl.Tryptophans were determinated by High Performance Liquid Chromatography with pH 4.5 NaAc buffer and 15% methanol as mobile phase. This method is simple, rapid and accurate. Its recovery is between 97.7%-104.1%.
【CateGory Index】: S816.11,Q517
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【References】
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1 WU Cai-lian,GUO Chang-jiang,YANG ji-jun,WEI jing-yu,XU Qi-shou(Institute of Hygiene and Environmental Medicine,Academy of Military Medical Science,Tianjin 300050,China);Separaton and Purification of L-Tryptophan from Fermentation Liquor[J];Amino Acids & Biotic Resources;2007-03
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1 ZHU Ying gui,\ MU Xiao long,\ WANG Lun,\ WANG Sheng qiong (College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, China);DETERMINATION OF 6-MERCAPTOPURINE WITH Cu(PHEN)_2~(2+)-H_2O_2-6-MERCAPTOPURINE SYSTEM BY CHEMILUMINESCENCE METHOD[J];Journal of Anhui Normal University(Natural Science);2003-01
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1 YANG Mei1,YAO Jun1,2 (1.College of Chemistry and Chemical Engineering,China West Normal University,Nanchong 637002;2.Department of Pharmacy,Chongqing Three Gorges Medical College,Wanzhou 404020);Electrochemical Behavior of L-Tryptophan at PolyMethyl Red Film Modified Electrode[J];Journal of Analytical Science;2010-03
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3 WANG Yi-bing,KE Ke,HE Bi-juan,ZHUANG Jun-lian,ZHANG Rong-can,XU Ming-ben(GX-ASEAN marine research center,Guangxi Academy of Sciences,Nanning 530007,Guangxi,China);Study on Zymohydrolysis Technology of Guangxi Ostrea Rivularis Gould[J];Food Research and Development;2011-05
4 CHEN Mei-lan1,YANG Xin-lei2,YE Ming-li2,ZHANG Li-juan2,LU Yan2 (1.College of Biology and Environmental Engineering,Zhejiang Shuren University,Hangzhou 310015,China;2.Shanghai Application Lab of Dionex China Co.,Ltd.,Shanghai 201203,China);Fast determination of amino acids in hydrolysate of leather and hair by HPLC with pre-column derivatization[J];Chinese Journal of Pharmaceutical Analysis;2011-05
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