Comparative Study of Expression and Mutations of Ras Gene in Patients with Non-small-cell Lung Cancer of Xuanwei and Kunming
YANG Hong-sheng, DENG Yong-jun, LI Ji-mei, CHAO Hu, SHI Yun-fei (Dept. of Thoracicsurgery, The 1st Affiliated Hospital of Kunming Medical College, Kunming 650032,China)
Objective: To compare the differences of ras gene alternations in non-small-cell lung cancer (NSCLC) between Xuanwei and Kunming . Methods: Samples of paraffin-embedded lung cancer tissues of 45 patients from Xuanwei and 45 patients from Kunming with NSCLC underwent radical resection were analyzed for expression of ras protein and mutations of k-ras codon 12 by immunohistochemica staining, polymerase chain reaction-single strained conformation polymorphism and DNA detect sequencing. Results: Among the 90 cases, 55 cases had ras protein expression and 28 cases had k-ras codon 12 mutations. Comparing the alternations of ras gene in 2 groups of NSCLC, the frequancy of ras protein expression and of k-ras mutation in Xuanwei group were higher than that in Kunming group, but no statistically significant difference was found. Except that there were some statistically significant differences between 2 groups in terms of expression of ras protein in the cases with stage I and the cases with adenocarcinoma, but no statistically significant difference in k-ras codon 12 mutation was found between 2 groups. Conclusion: These data suggest that the activation of ras gene is a commonly genetic event in the pathogenesis of NSCLC, the mutations of k-ras gene codon 12 are ubiquitous in 2 groups of NSCLC, there was statistically signifimant difference in ras protein expression in patients with stage I and with ademocarcinoma between 2 groups.