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《Journal of Kunming Medical University》 2015-07
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Comparison of Side Population Cells Level and Biological Characteristics between Human Breast Cancer Tissue and MCF-7 Cells

LI Jia;JIN Cong-guo;ZOU Tian-ning;CHEN Xiao-qun;LIU Xin;WANG Xi;WANG Xi-cai;Tumor Research Center,Yunnan Tumor Hospital;Dept. of Mastopathy,Yunnan Tumor Hospital;  
Objective To compare side population(SP) cells level and biological characteristics between human breast cancer tissue and MCF-7 cells.Methods Altra flow cytometry was used to isolate SP cells from human breast cancer tissue and MCF-7 cells with Hoechst 33342 dye efflux and analyse the SP cells level as well as the expression of cells markers and cell cycles.Results The proportions of SP cells in human breast cancer tissue and MCF-7 cells were(4.68±0.91)% and(12.43±1.68)%.The proportion of SP cells in MCF-7 cells was significantly higher than that in human breast cancer tissue.The expression of SP cells biological marks(ABCG2, CD133, CD43, CD24, Ki-67, MDR) and the cells cycle(G0/G1,S,G2/M,apoptosis rate, necrosis rate) were similar in both human breast cancer tissue and MCF-7 cells.Conclusion Side population cells were detected in human breast cancer tissue and MCF-7 cells, and proportions of SP cells in the two sources were different.However, both sources' SP cells were provided with the characteristics of cancer stem cells. Altra flow cytometry analysis method provided a certain frame of reference in identification and isolation the breast cancer stem cells.
【Fund】: 云南省卫生厅科学研究基金资助项目(2011WS0067)
【CateGory Index】: R737.9
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