Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Stomatology》 2005-03
Add to Favorite Get Latest Update

Growth characteristics of cultured adult bone marrow stromal cells in vitro

XIE Qian, LUO Li, HUANG Hong-zhang.(Department of Clinical Medicine, Guangdong College of Pharmacy, Guangzhou 510224, China)  
Objective To investigate the growth characteristics of cultured adult bone marrow stromal cells (MSC) in vitro to provide the experimental basis of MSC used as the functional cells in bone tissue engineering. Methods Bone marrow was obtained by aspirated in the posterior superior iliac spine or flushed medullary cavity in costal bone from an adult patient whose marrow was normal. Mononuclear cells were then isolated by centrifugation for primary culture in different culture fluid systems. Subculture was done for 8 passages consecutively. The following data were obtained: growth curve, division rate, adhesive rate and double time. Results The growth curves of the third and sixth passages were very similar. The highest division rate of the third passage was 15‰. 88.54% of the subcultured cells were adhesive in 12 h. Conclusion MSC showed stable growth, relatively rapid proliferation and easy survival in the subculture in present culture condition. MSC in adults may be used in autogeneous bone engineering.
【CateGory Index】: R329.2
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 1 Hits
1 WANG Wan-Ming, HU Yun-Yu, ZHAO Guang-Yue, LU Rang, YANG GuangInstitute of Orthopaedics, Xijing Hospital, Fourth Military Medical University, Xi'an 710033;Investigation of the growth characteristics of cultured rabbit marrow stromal cells[J];Journal of The Fourth Military Medical University;1998-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LU Bing Lun 1, LIU Bao Lin 2, FENG Zhi Hua 3,HONG Yong Long 2 1Department of Plastic Surgery, Xijing Hospital, 2Department of Oral and Maxillofacial Surgery, Qindu Stomatological College, 3Department of Infectious Disease,Tangdu Hospital;Modification culture in osteoblasts from human bone marrow and osteogenic potential of stromal cell in human bone marrow in vitro[J];JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY;1999-11
2 LIU Dan Ping 1, HU Yun Yu 1, SHI Xin You 2, SUN Long 1, MA Sheng Bing 1 1Institute of Orthopedics of Chinese PLA, Xijing Hospital, 2Laboratory Aminal Research Center, Fourth Military Medical University, Xi'an 710033, China;Aspiration of rabbit bone marrow by bone puncture[J];JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY;2000-06
3 ZHENG Lei WANG Qian WEI Kuan-hai Pei Guo-xian (Department of Orthopedics and Traumatology. Clinical Laboratory Nanfang Hospital, First Military Medical University. Guangzhou 510515. China);Biological effects of basic fibroblast growth factor on bone marrow stromal cells[J];JOURNAL OF FIRST MILITARY MEDICAL UNIVERSITY;2000-02
4 HUANG You-zhang~1,SHEN Jian-liang~1,YANG Ping-di~1,XU Shi-xia~2,WU Nan-hai~3,XIANG Dan~1,GONG Li-zhong~1,CEN Jian~1,WANG Li-xin~1,ZHENG Pei-hao~1(1.Department of Hematology,Naval General Hospital,Beijing 100037,China;2.Department of Information,Naval General Hospital,Beijing 100037,China;3.Department of Pediatrics,Naval General Hospital,Beijing 100037,China);Cryopreservation of Hemotopoietic Stem Cells Using Dimethyl Suflfoxide and Hydroxyethyl Starch in Low Temperature Refrigerator and Autocontrolled Programmed Cryogenic[J];Journal of Naval General Hospital;2006-03
5 Qian Qichun, Liu Yanpu, Chen Fulin et al. Stomatological College, Fourth Military Medical University, Xi'an 710032;BONE RECONSTRUCTED BY SEEDING HUMAN BONE MARROW STROMAL CELLS ON POROUS CERAMIC[J];Medical Journal of Chinese People's Liberation Army;2002-04
6 ZHENG Lei;WANG Qian (Dept.of Medical Examination, Nanfang Hospital, The First Mi litary Medical University, Guangzhou 510515, China);Biological effects of basic fibroblast growth factor on bone marrow stromal cells[J];JOURNAL OF JINAN UNIVERSITY(NATURAL SCIENCE & MEDICINE EDITION);2000-01
7 WEI Kuan\|hai, PEI Guo\|xian\+*, ZHENG Lei, JIN Dan, WANG Qian, MA Zhong\|li (Department of Orthopedics and Traumatology, Nanfang Hospital, the First Military Medical University, Guangzhou\ 510515, China) \;[;THE EFFECTS OF BASIC FIBROBLAST GROWTH FACTOR AND DEXAMETHASONE ON PROLIFERATION AND DIFFERENTIATION OF BONE MARROW STROMAL CELLS IN SKELETAL SYSTEM[J];Acta Anatomica Sinica;2002-02
8 XIE Qian,Luo Li. Department of oral-maxilla-facial surgery, the second affiliated hospital of Sun Yet-sen University,Guangzhou 510120;Investigation of Biological Characteristics and Culture of Stromal Cells of Human Bone Marrow in Vitro[J];Journal of Comprehensive Stomatology;2004-02
9 CAO Zhiqiang,DING Yijian,WANG Huiming,et al The1st Affiliated Hospital of Medical College Zhejiang Unversity,Hangzhou,310006;Experimental Study of the osteogenesis of cultured osteoblast derived from the rabbit marrow in vivo[J];Chinese Journal of Oral Implantology;2003-01
10 ZHANG Xiao-dong,YANG Wei-dong,SONG Jiu-yu,QIAN Qi-chun,GU Xiao-ming,CHEN Fu-lin.Department of Stomatology,Shenyang Military General Hospital,Shenyang110015,China;The experimental study on the effect of repairing mandibular defects with BMSCs/ cancellous bone matrix composite strengthened by titanium mesh[J];Journal of Clinical Stomatology;2003-10
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Luo Chengji. The Third Military Medical College, Chongqing 400038;THE SURVEY AND PERSPECTIVE OF STROMAL CELLS IN BONEMARROWHEMATOPOIETICINDUCTIVEMICRO ENVIRONMENT[J];MEDICAL JOURNAL OF CHINESE PEOPLE'S LIBERATION ARMY;1999-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved