Effects of acidic fibroblast growth factor and transforming growth factor-β1 on proliferation of porcine dental papilla cells
YU Shan-shan,JIANG Xin-quan,WENG Yu-lai,HUA Fei.(Department of Oral Medicine,Ninth People's Hospital,School of Medicine,Shanghai Jiaotong University,Shanghai Key Lab of Stomatology,Shanghai Institute of Stomatology,Shanghai 200011,China)
Objective To investigate the effects of acidic fibroblast growth factor and transforming growth factor-β1 on the proliferation of porcine dental papilla cells. Methods Isolated porcine dental papilla cells were cultured in DMEM/F12 supplemented with aFGF and TGF β1. MTT colorimetric method was used to assess dose-effect and time-effect of aFGF and TGF β1 on the proliferation of pDPCs.All statistical analyses were performed with one-way ANOVA. Results Within 8 days,aFGF could significantly promote the proliferation of pDPCs with the concentrations between 5 ng/ml and 100 ng/ml,especially with the concentration of 10 ng/ml and 25 ng/ml,while the proliferation effects of TGF β1 sharply decreased at the dose varying from 5 ng/ml to 50 ng/ml. The effect was the strongest for the proliferation of pDPCs with the combination of 25 ng/ml aFGF and 10 ng/ml TGF β1. Conclusion The results suggested that in certain conditions,aFGF enhanced the proliferation of pDPCs while TGF β1 inhibited the proliferation . However,the promotive effects were achieved when the growth factors were used in combination. Supported by National 973 Project ( G1999054308 ).