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《Wetland Science》 2014-05
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Phylogeny of Psychrophilic Bacteria from Peat Swamp Soil in Zoigê Plateau

GU Yun-fu;ZHENG You-kun;Petri Penttinen;WANG Xian-bin;ZHANG Xiao-ping;Department of Microbiology,College of Resource and Environment,Sichuan Agricultural University;Department of Food and Environmental Sciences,University of Helsinki;  
The samples at depth of 10-20 cm of peat swamp soil, covered by Carex meyeriana, were collected from the pastures in Axirong village, Maixi village and Military subarea in Zoigê Plateau in October 2008,and 9 sampling sites were arranged in each sampling pasture. To isolate cold-adapted bacterial strains from the peat swamp soil, bacteria were enriched using aerobic heterotrophic conditions at 4 ℃. Then amplified ribosomal DNA restriction analysis(ARDRA) was applied to analysis phylogeny of the isolates, which could be further used to monitor the potential biotechnical applications of the specific enzymes produced by these bacteria. Totally, 47 strains with different phenotypic characteristics were isolated from the three areas. The majority of the isolates were phylogenetically related to bacteria isolated from various cold environments such as the two Polar Regions, permafrost and glaciers. The isolates represented 4 major groups: the high-GC and the low-GC Gram-positives, and β- and γ-Proteobacteria. Among them, 4 representative isolates related(97.0%) to the genus Arthrobacter. REGD2 that represented the biggest ARDRA group was most closely related to Arthrobacter sp. GW10-3 isolated from Antarctica, with 99.46% sequence similarity; three isolates, REGD7,REGD15, and REGD16, were highly similar to Arthrobacter sp. strains isolated from Arctic, Antarctic and Himalaya; two representative isolates were grouped in the low-GC Gram-positives: REGD5 was most closely related to Exiguobacterium sibiricum strain 255-15 isolated from Siberian permafrost, with 99.6% sequence similarity; the most similar cultured strain to the other low-GC isolate, REGD8, was Paenisporosarcina macmurdoensis strain HWG-A8 isolated from the Tibetan Plateau permafrost soil, with 99.8% sequence similarity. The Gram-negative isolates are all belong to Proteobacteria, two representative isolates were affiliated with β-Proteobacteria while the other seven representative isolates related to γ-Proteobacteria. REGD10 was most similar to Variovorax paradoxus strain IHB B 1313 isolated from the cold deserts of the Indian trans-Himalayas,with 99.4 % sequence similarity, while REGD11 was most closely related to Janthinobacterium sp. HC3-10 from Arctic Cyanobacterial mats, with 99.8% sequence similarity. Five isolates(REGD1, REGD6, REGD9,REGD12, and REGD14) were affiliated with different Pseudomonas strains. The other two isolates(REGD3,REGD4) were affiliated with the genus Stenotrophomonas. However, the most similar cultured strain to both these isolates was Acetobacter pasteurianus strain CCM 3606 with 99.74% and 99.8% sequence similarity, respectively. Most of the isolates(87%) from the plateau wetland soil deposits were psychrotrophic and could not grow at temperatures above 30 ℃, and their optimum growth temperature were between 10-15 ℃. Most of the isolates could produce cellulase and almost half of them showed protease activity. In the extracellular enzyme assays, the Arthrobacter isolates were the most versatile ones. The wide range of enzymatic activities of the isolates suggested that psychrophilic bacteria in Zoigê Plateau are a promising source of enzymes for biotechnical applications.
【Fund】: 四川农业大学双支计划项目;; 四川省教育厅青年基金项目;; 国家自然科学基金项目(41201256)资助
【CateGory Index】: Q93
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Chinese Journal Full-text Database 5 Hits
1 CHEN Zhi-Ke1,2,LU Xian-Guo1 (1. Key Laboratory of Wetland Ecology and Enviroment of Chinese Academy of Sciences,Northeast Institute of Geography and Agroecology,Chinese Academy of Sciences,Changchun 130012,Jilin,P. R. China; 2. Graduate University of Chinese Academy of Sciences,Beijing 100049,P. R. China);Comparison between the Marsh Wetland Landscape Patterns in the Zoigê Plateau for Two Periods[J];Wetland Science;2010-01
2 JIANG Jin-gang 1,2,3,LI Ai-nong 1,BIAN Jin-hu 1,YU Zhi-feng 2,3,LEI Guang-bin 1(1.Institute of Mountain Hazards and Environment,Chinese Academy of Sciences,Chengdu 610041,Sichuan,P.R.China;2.Institute of Remote Sensing and Earth Sciences,Hangzhou Normal University,Hangzhou 311121,Zhejiang,P.R.China;3.Zhejiang Provincial Key Laboratory of Urban Wetland and Regional Change,Hangzhou 311121,Zhejiang,P.R.China);Change of Wetlands in Zoigê County from 1974 to 2007[J];Wetland Science;2012-03
3 FEI Shi-min~1 CUI Li-juan~2 HE Ya-ping~1 CHEN Xiu-ming~1 JIANG Jun-ming~1(1.Sichuan Academy of Forestry,Chengdu 610081;2.Chinese Academy of Forestry,Beijing 10091);A Background Study of the Wetland Ecosystem Research Station in the Ruoergai Plateau[J];Journal of Sichuan Forestry Science and Technology;2006-02
4 ZHANG Ke,CHEN Qiang,ZHAO Ke,WANG Wenyue,ZHU Xuemei College of Resource and Environmental Sciences,Sichuan Agricultural University,Yaan 625000,Sichuan,China;16S rDNA PCR-RFLP analysis and phylogeny of bacteria isolated from swamp and meadow aeolian soils in the Zoige Plateau,Sichuan,China[J];Acta Ecologica Sinica;2010-12
5 TANG Jie XU Qing-Rui WANG Li-Ming DING Xiang TANG Bo WU Li-Sha FENG Su SUN Qun YANG Zhi-Rong ZHANG Jie*(Key Laboratory of Bio-resources and Bio-environment,Ministry of Education,College of Life Sciences,Sichuan University,Chengdu,Sichuan 610064,China);Soil bacterial community diversity under different stages of degradation in zoige wetland[J];Microbiology China;2011-05
Chinese Journal Full-text Database 10 Hits
1 LI Xiang-yun,SONG Chao-yu,WANG Rui-ying,WANG Sheng-jian,GAO Jun-ling,ZHU Pi-sheng(Qingdao Academy of Agricultural Sciences,Qingdao 266100,China);Effects of Different Animal Manure Amount on Cauliflower Growth and Soil Fertility[J];Shandong Agricultural Sciences;2008-03
2 WU Huan-tao,WEI Min,YANG Feng-juan,WANG Xiu-feng(College of Horticulture Science and Engineering,Shandong Agricultural University/State Key Laboratory of Crop Biology,Taian 271018,China);Improving Effects of Crop Rotation and Fallowing on Continuous Cucumber Cropping Soil in Solar Greenhouse[J];Shandong Agricultural Sciences;2008-05
3 ZHAO Ming,WANG Wen-jiao,CAI Kui,ZHAO Zheng-yu,YU Qiu-hua(Qingdao Academy of Agricultural Sciences,Qingdao 266100,China);Water-soluble Fluorine Content in Laoshan Green Tea and Its Influencing Factors[J];Shandong Agricultural Sciences;2008-09
4 ZHANG Ying-peng,LI Yan,ZHANG Yu-feng,DONG Liang,SUN Ming(Soil and Fertilizer Institute,Shandong Academy of Agricultural Sciences,Jinan 250100,China);Effects of Water-soluble Chelate Fertilizer on Yield and Nutrient Content of Chinese Onion of Zhangqiu[J];Shandong Agricultural Sciences;2009-04
5 TIAN Ye,LI Yan,SUN Ming,ZHANG Ying-peng*(Soil and Fertilizer Institute,Shandong Academy of Agricultural Sciences,Jinan 250100,China);Effects of Chelate Liquid Fertilizer Application on Yield and Quality of Tomato and Physical and Chemical Characters of Soil in Plastic Greenhouse[J];Shandong Agricultural Sciences;2009-11
6 ZHAO Ming,CAI Kui,WANG Wen-jiao,ZHAO Zheng-yu,CHEN Jian-mei(Qingdao Academy of Agricultural Sciences,Qingdao 266100,China);Effects of Combined Application of Organic and Inorganic Fertilizer on Yield and Quality of Tomato[J];Shandong Agricultural Sciences;2009-12
7 WANG Ke-an1,YANG Ning1,LI Xu-hua2,GUO Pei-qiu2,Lü Xiao-hui1,CHAI Xiu-qian1(1.Vegetable Institute,Shandong Academy of Agricultural Sciences/ Key Laboratory for Biology of Greenhouse Vegetable/Shandong Branch of National Improvement Center for Vegetable,Jinan 250100,China;2.Shandong Agricultural University,Taian 271018,China);Variation Laws of Soil Nutrients in Solar Greenhouses with Different Cultivation Ages[J];Shandong Agricultural Sciences;2010-09
8 DONG Xiao-xia1,SUN Ze-qiang1,ZHANG Li-ming2,WANG Xue-jun1,WANG Qing-mei3,XIE Bei-tao3,ZHENG Dong-feng1(1.Soil & Fertilizer Institute of Shandong Academy of Agricultural Sciences,Jinan 250100,China;2.Shandong Academy of Agricultural Sciences,Jinan 250100,China;3.Crop Institute of Shandong Academy of Agricultural Sciences,Jinan 250100,China);Fertilizer Utilization Efficiency of Sweet Potato in Main Types of Soil in Shandong Province[J];Shandong Agricultural Sciences;2010-11
9 WANG Wei1,YUAN Liang2 (1.Dezhou Academy of Agricultural Sciences,Dezhou 253015,China;2.Institute of Agricultural Resources and Regional Planning,CAAS,Beijing 100081,China);Change Rule of Microbial Biomass Nitrogen in Sheltered Soil and Its Relation with Soil Fertility[J];Shandong Agricultural Sciences;2011-04
10 ZHOU Hai-yan1,WU De-min1,LI Yan2,ZHANG Ying-peng2,LIU Zhao-hui2,JIANG Li-hua2(1.Zhaoyuan Agricultural Technique Extension Center,Zhaoyuan 265400,China;2.Institute of Agricultural Resources and Environment,Shandong Academy of Agricultural Sciences/Shandong ProvincialKey Laboratory of Plant Nutrition and Fertilizer/Shandong Provincial Key Laboratory of Agricultural Non-Point Source Pollution Control,Jinan 250100,China);Effects of Nitrogen Application on Yield,Agronomic Characters and Nitrogen Utilization Efficiency of Winter Wheat with Straw Returning[J];Shandong Agricultural Sciences;2011-05
China Proceedings of conference Full-text Database 10 Hits
1 Li Xiao-liang~(1,2) Li Jian-jiao~1 L(u|¨) Bao-lei~1 Chen Xiao-min~2 Wang Xiao-yang~2 Zhan Guofei~1 (1 College of Urban Construction and Environmental Sciences,Anhui Science and Technology University,Fengyang 233100,China;2 College of Resources and Environmental Sciences,Nanjing Agricultural University,Nanjing 210095,China);Effects of Conjunctive Application of Fertilizer and Gypsum on the Biological Properties of Potting Corn in Coastal Saline Soil[A];[C];2011
2 Yuan Man-man~1,Ye Shu-ya~1,Liu Feng~1,Li Min~1,Wu Xue-zhong~2 (1.Institute of Soil and Fertilizer,Anhui Academy of Agricultural Sciences,HefeiAnhui 230031;2.Wuhu Agricultural Technology Popularization Center,Wuhu Anhui 241000);Different Controlled-release Nitrogen Fertilizer on Yield and Nitrogen Utilization Rate of Maize[A];[C];2011
3 JIANG Guang-yue,GUO Xi-sheng,ZHU hong-bin,HE Chuan-long (Soil and Fertilizer Institute,Anhui Academy of Agricultural Science,Hefei,Anhui 230031);Environmental Quality Evaluation with 7 Heavy Metals of Greenhouse Soil in Hefei City[A];[C];2011
4 JIA bo~(1,2),ZHANG Hou-hu~2,ZHANG Yi~2,WANG Wei~2,WURui2,ZHANG Wei-jia~1 (1.Suzhou Unversity of Science and Technology,School of Environmental Science and Engineering,Suzhou, Jiangsu,215011,China.2.Nanjing Institute of Environmental Sciences,Ministry of Environmental Protection, Jiangsu Nanjing 210042);Effects of Livestock Wastewater NH_4~+-N Incubation on Landfill CH_4 Oxidation by Mineralized Refuse Particle[A];[C];2011
5 LIU Kefeng,WANG Hongli,ZOU Rongsong,SHI Aiping,DAI Zhiyong (Beijing University of agriculture,Beijing,102206);Study on the Best Production Condition of Pig Manure Compost[A];[C];2008
6 LI Hao,MA Wan-li,Wu Yong-sheng,LV Gui-fen (College of life Sciences and Technology,Inner Mongolia Normal University,Huhhot 010022,China);Research on dynamic changes of soil Microbial biomass,soil respiratory intensity in desert grassland of Inner Mongolia[A];[C];2008
7 WANG Shunli~1,JIN Zhulida~1,LIU Kefeng~(1*),ZOU Rongsong~2, WANG Hongli~1,SHI Aiping~1,XING Guangqing~1 (1.Beijing University of agriculture,Beijing,102206; 2.Shenzhou biotechnology company,Inner Mongolia,010200,China);Study on the Influences of Strawberry Growth and Fruit Quality Treated by Different Microbe Organic Fertilizers[A];[C];2008
8 JIN Jian~1 WANG Guang-hua~1 LIU Xiao-bing~1 ZHANG Qiu-ying~1 XU Yan-xia~1 LIU Jun-jie~1 Stephen J Herbert~2 (1.Key lab of black soil,North-east Institute of Geography and Ecological Agriculture,CAS,Harbin 150081,Heilongjiang;2.Department of Plant and Soil Sciences,University of Massachusetts,Amherst 01003,MA,USA);Effect of water-phosphorus interaction on root morphology,yield and quality in soybean[A];[C];2007
9 ;Study on the Cause of Leaf Tipburn,Torpid and Withered,Yellow of Outside Leaf in Bunching Onion[A];[C];2008
10 Zhang Shuqing, Liu Xiumei, Feng Zhaobin ( Institute of soil and fertilizer, Chinese Academy of Agriculture Science, Beijing, 100081 Institute of soil and fertilizer, Jiangxi Academy of Agricultural Science, Nanchang, 330200);Characteristics of Adsorption and Desorption of Humic Acids Made from Airslake-coal to Nitrogen, Phosphorus and Potash[A];[C];2006
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 XU Hui-xi1,HE Zheng-wei1,DAN Shang-ming2,DAN Bo3,YANG Cun-jian4(1.Institute of Digital Land & Ecological Science,Chengdu University of Technology,Chengdu Sichuan 610072,China;2.Sichuan Province Agrimeteorological Center,Chengdu Sichuan 610072,China;3.Sichuan Provincial Meteorological Observatory,Chengdu Sichuan 610072,China;4.Sichuan Software Key Laboratory,Sichuan Normal University,Chengdu Sichuan 610068,China);The Wetlands in Zoig Plateau Derived from Quantitative Remote Sensing Based on EOS/MODIS[J];Journal of Glaciology and Geocryology;2007-03
2 YANG Xia 1,ZAI Xing-li 2,YU Guo-ying 3 (1.Vocational and Technological Institute,Changchun University,Changchun?130022,China;2.Biological Department,Henan Shangqiu Teachers University,Shangqiu?476000,China;3.Changchun Geographical Research Institute;Ch;Current stuation of Roige plateau wetlands biodiversity and their conservation countermeasure[J];Journal of Changchun University;2002-03
3 WANG Hui-ying1,SU Wei2,ZHOU Jun-qi1(1.School of Remote Sensing and Information Engineering,Wuhan University,Wuhan 430079,China;2.College of Information and Electrical Engineering,China Agricultural University,Beijing 100083,China);Object-oriented information extraction method of remote sensing image based on multi-classifier mixed[J];Engineering of Surveying and Mapping;2009-05
4 JIA Ping①,LI Hai-tao②,LIN Hui③,GU Hai-yan②,HAN Yan-shun②(①Information Center of Ministry of Land and Resources of People's Republic of China,Beijing 100812,China;②Institute of Photogrammetry and Remote Sensing,Chinese Academy of Surveying and Mapping,Beijing 100039,China;③Department of Territory Resource Information and Surveying Engineering of XuZhou Normal University,Jiangsu,Xuzhou221116,China);Research on multi-source remote sensing image classification based on SVM[J];Science of Surveying and Mapping;2008-04
5 HOUZhong,LI Yuanhua,FANGPing(Sichuan Grassland tation Chengdu,Chengdu 610041,China);Analysis on the distribution regularity of spinal animals in Ruoergai[J];Pratacultural Science;2003-03
6 LI Wen-long,WANG Jing,GUO Shu-mao,WANG Ying (College of Pastoral Agriculture Science and Technology,Lanzhou University,Lanzhou 730020,China);Dynamic change of marsh landscape patterns and its driving forces in Maqu[J];Pratacultural Science;2009-08
7 Liu Shigui;Long Zhangfu ;Ge Shaorong(Department of Biotechnology,Sichuan University,Chengdu,Sichuan 610064);Studies on Soil Microorganism Numbers and Microbiota of Degenerated Rangelands in Northwest Region of Sichuan P R.China[J];ACTA PRATACULTURAL SCIENCE;1994-04
8 LIU Hongyu,LU Xian-guo,ZHANG Shi-kui(Northeast Institute of Geography and Agricultural Ecology,CAS,Changchun 130012);Progress on the Study of Process of Wetland Landscape Changes and Cumulative Environmental Effects[J];Progress In Geography;2003-01
9 BAI Junhong, OUYANG Hua, YANG Zhifeng, CUI Baoshan, CUI Lijuan, WANG Qinggai ( Environmental School, Beijing Normal University, Beijing 100875; Institute of Geographical Science and Natural Resources Research, CAS, Beijing 100101; Chinese Academy of forestry, Beijing, 100081; Appraisal Center for Environment and Engineering, State Environmental Protection Administration, Beijing 100012);Changes in Wetland Landscape Patterns:a Review[J];Progress In Geography;2005-04
10 ZHU Huiyi, LI Xiubin(Institute of Geographic Sciences and Natural Resources Research, CAS, Beijing 100101, China);Discussion on the Index Method of Regional Land Use Change[J];Acta Geographica Sinica;2003-05
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