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《Journal of Clinical Pediatrics》 2007-07
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Effects of Lonicera on the immunoregulation of intestinal mucosal inflammatory response and sIgA response in ovalbumin sensitized BALB/c mice

LI Fei,LI Hai-qi(Department of Primary Child Care,Children's Hospital of Chongqing Medical University,Chongqing 400014,China)  
Objectives To evaluate the therapeutic effects of Lonicera extract from water solution(Lon ext)on the intestinal mucosal inflammatory response and sIgA response in ovalbumin(OVA)sensitized BALB/c mice.Me-thods Female BALB/c mice aged at 6 weeks fed on OVA-free diet,were randomly divided into 5 groups by the concentration of drugs used in intragastric treatments.Eight mice were included in each group with 100%(H),50%(M),25%(L),of the Lon ext and no drug(Ch)administered after being sensitized and challenged with OVA.The mice receiving normal saline(NS)without sensitization and challenge were set as the negative control.The levels of total IgA and OVA-specific IgA(OVA-IgA)in the intestinal mucus of the mice were determined by ELISA.The number of IgA+ plasmacytes in Lamina propria(LP)and IgA+ lymphocytes on the surface membrane(sm)of Peyer's patches(PP)were determined by immunohistochemistry.The IL-4 mRNA expression was determined by RT-PCR.In situ hybridization was used to determine the mRNA expression of IL-4 in small intestine PP,and TGF-β1 and TNF-α in intestinal mucosa.Results When the allergic mice were treated with the H or M Lonicera extract,the total and OVA-specific IgA secreted in intestinal mucsa was suppressed.The amount of IgA+ plasmacytes in LP and the smIgA+ lymphocytes in PP and the mRNA expression of IL-4 in LP and in PP were reduced,while the mRNA expression of TGF-β1 was notably upregulated(P 0.01=.In group H,M and L,Lon ext reduced TNF-α mRNA expression in a dose-dependent manner(P 0.01=.Conclusions The intestinal mucosal inflammatory responses in the ovalbumin-induced allergic mice may be related to the imbalance of anti-inflammatory and pro-inflammatory cytokines.Lon ext can be used to treat intestinal mucosal inflammation in ovalbumin sensitized mice.
【Fund】: 重庆市卫生局资助项目(No.渝中医[2002]42号)
【CateGory Index】: R285.5
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