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Experimental study on delaying atherosclerotic plaque formation in rabbit by Huashi Tongyu Buqi method

XU Rui-ping;YU Hai-xiang;Binay Kumar Adhikari;MENG Xiao-ping;Department of Cardiology,the Second Hospital of Jilin University;Department of Thoracic Surgery,the China-Japan Union Hospital of Jilin University;  
Objective The study was to observe the delaying of atherosclerotic(AS)plaque formation in rabbit fed with high lipid diet by Huashi Tongyu Buqi Method.Methods Pure bred 18New Zealand male white rabbits(4months old)were experimental subjects.After normal feeding for one week,the rats were changed to high lipid diet(1% cholesterol+ 6% yolk powder + 8% lard +85% basic fodder),and they were randomly divided into three groups:six rabbits in control group(without using any drugs),six rabbits in Chinese medicine group(Heye,Zexie,Huzhang,etc)and six rabbits in western medicine group(aspirin+ atorvastatin+ probucol)and observed for three months.After three months,auricular venous blood of each group was drawn and total cholesterol(TC),triglycerides(TG),low-density lipoprotein-cholesterol(LDL-C),serum matrix metalloprotinase-9(MMP-9),malondialdehyde(MDA)and ox-LDL were measured,and then rabbits were killed to get aorta.Hematoxylin-eosinstaining and immunohistochemistry staining were used to observe the histopathological changes of aorta,atherosclerotic plaque area,intima and vessel wall thickness.Results TC,TG and LDL-C of Chinese medicine group and western medicine group were significantly lower than those of control group(P0.01),respectively TC(2.39±0.15)mmol/L,(2.08±0.12)mmol/L vs(13.02±0.20)mmol/L;TG(2.58±0.16)mmol/L,(1.90±0.08)mmol/L vs(11.08±0.19)mmol/L;LDL-C(1.05±0.24)mmol/L,(0.85±0.09)mmol/L vs(10.13±0.20)mmol/L,TC and TG of western medicine group was much lower than that of Chinese medicine group(P0.01);MDA,MMP-9and ox-LDL level as compared with control were decreased by different extents(P 0.01).Aortic intima thickness of Chinese medicine group and western medicine group decreased significantly as compared with that of control group,(12.28±1.28)μm,(5.20±0.81)μm vs(40.06±3.94)μm(P 0.01),while aortic intima thickness of western medicine group showed more decrease.Conclusion Huashi Tongyu Buqi method delays the atherosclerotic plaque formation in rabbit,decreases blood LDL-C,provides new research theoretical evidence for Chinese medicine and atherosclerosis.
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