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《Journal of Clinical Pulmonary Medicine》 2009-03
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Study on Shuanghuanglian inhibiting effect of influenza virus A1 gene

SUN Jian,WANG Nong-rong,YANG Bin,HE Shi-qing.The Fourth Hospital Affiliated to Nanchang University,Nanchang 330003,China  
Objective To establish a real-time fluorogenic quantitative RT-PCR method for detecting the expression of PA gene of influenza virus A1 gene and to investigate the inhibitore effect of Shuanghuanglian.Methods Real-time fluorogenic quantitative RT-PCR(FQ-RT-PCR)was set up to examine the specific expression of test group and virus control group PA gene in virus.Results After 7 duplicate tests with the FQ-RT-PCR.method,the average level of the virus control group was 3.62×106±0.90 copies/μg RNA in virus and 4.57×105±1.23 copies/μg RNA in virus test group.Virus control group was significantly higher than the test group(P0.001).Conclusion This showe that Shuanghuanglian could inhibit PA gene expression of influenza virus A1.
【Fund】: 江西省自然科学基金资助项目
【CateGory Index】: R285.5
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【References】
Chinese Journal Full-text Database 3 Hits
1 FU Song-zhe,NI Xian-sheng,XIA Wen,et al.(Nanchang Municipal Center for Disease Control and Prevention,Nanchang 330038,Jiangxi,P.R.China);Comparison of three RNA extraction kits for detection of H1N1 influenza virus nucleic acids[J];China Tropical Medicine;2010-07
2 WANG Linlin,SHI Yuzhu,WANG Xue,LIU Yan,YANG Qiaoli,YAO Hua,HUANG Hua(Institute of Materia Medica of Xinjiang,Urumqi 830004,China);Progress of anti-influenza virus by Chinese herbs[J];Northwest Pharmaceutical Journal;2012-06
3 WANG Xue YANG Qiaoli SHI Yuzhu LIU Yan WANG Linlin YAO Hua HUANG Hua Institute of Materia Medic,Xinjiang Uygur Autonomous Region,Urumqi 830004,China;Research progresses of Chinese herbs on anti-influenza virus[J];China Medical Herald;2012-33
【Citations】
Chinese Journal Full-text Database 3 Hits
1 YI Shi hong 1,WANG Fang 1,WANG Li ping 2,ZHAO Chun yan *,WEI Qiang 1,LI Fan 1 (1\ Department of Pathogenobiology,School of Basic Medical Sciences,Jilin University, Changchun 130021 China;\ 2\ Department of Pathology,First Hospital,Jilin Univers;Curative effect of Shuanghuanglian injection on anti-virus in vitro[J];Journal of Norman Bethune University of Medical Science;2001-05
2 MA Shuang - cheng, Paul BUT Pui - Hay, Vincent OOI Eng - Chun, Spencer Hon - Sun LEE , Song - Fong LEE (National Institute for the Control of Pharmaceutical & Biological Products,Beijing 100050,China; Department of Biology and Institute of Chinese Medicine,The Chinese University of Hong Kong,Shatin,Hong Kong,China; Department of Microbiology and Immunology, Dalhousie University, Halifax, Canada);Determination of the Antiviral Caffeoyl Quinic Acids Isolated from Lonicera japonica Thunb.[J];Chinese Journal of Pharmaceutical Analysis;2005-07
3 ZHANG Wei 1,LU Fang-guo2,ZHOU xing2(Hunan Agricultural University,Changsha 410200 ;Hunan University of Traditional Chinese Medicine,Changsha 410007);The Expreimental Study Advaces and think on Anti-influenza Virus of Traditional Chinese Medicine[J];China Practical Medicine;2007-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Chen Baofeng1 Zhu Gaopu2* Zhang Shulin2 Meng Li3(1 Medical Biology Depart of North Sichuan Medical College,Nanchong,Sichuan 637007;2 Agriculture College of Guangxi University,Nanning,Guangxi 53004;3 Henan Institute of Science and Technology,Xinxiang,Henan 453003);Filtrate Culture Medium of Zn~(2+)-Honeysuckle bine of Chaxingu[J];Anhui Agricultural Science Bulletin;2007-19
2 CHEN Wen(Medical education research department,Huizhou Medical School,Huizhou,Guangdong 516002,China);Research on selecting the best extraction methods of chlorogenic acid in stems and leaves of L.confusa DC[J];Journal of Chengdu Medical College;2011-01
3 SHEN Zhi1,ZHANG Wen-ting2,HUA Yun-fei1,ZHAO Wei-liang2 1.College of Pharmacy,Zhejiang University of Traditional Chinese Medicine,Hangzhou 310053,China 2.Zhejiang Institute for Food and Drug Control,Hangzhou 310004,China;Fingerprinting Analysis of Four Variants of Chrysanthemi Morifoli Flos by RP-HPLC[J];中草药(英文版);2010-02
4 WANG Xue-bing,WEI Zhan-yong,CUI Bao-an,LI Chang-lei,XU Duan-hong,CHEN Hong-ying(Engineering College of Animal and Vetrinary Science,Henan Agricultural University,Zhengzhou 450002,China);Extraction of Chlorogenic Acid from Chinese Herbal Medicine and its Antiviral Effect on Porcine Parvovirus in Vitro[J];China Animal Husbandry & Veterinary Medicine;2008-12
5 YU Yan,SHI Wei-jing*,DENG Ja-sheng,ZHAN Ping,ZHANG Dong-sheng,LIU Li-jun,LI Meng(College of Food and Drug,Anhui Science and Technology,Fengyang 233100,China);Simultaneous Determination of Seven Kinds of Compounds in Flos Chrysanthemi by HPLC[J];Journal of Anhui Science and Technology University;2012-05
6 CHU Xiu-ling1,SU Jian-qing1,WEI Xu-bin2(1.College of Agronomy,Liaocheng University,Liaocheng 252000,Shandong,China;2.College of Animal Husbandry and Veterinary,Jilin University,Changchun 130062,China);Research Progress and Ideas on Antivirus Chinese Traditional Medicine[J];Hubei Agricultural Sciences;2009-05
7 Zeng Xiangfeng,Zeng Yaoying,Li Haixian,Lin Changle,Li LipingThe Jinan University Key Laboratory of Ministry of Education for Tissue Transplantation and Immunology,Guangzhou,Guangdong,China,510632;Experimental Study on the Influence of Shuanghuanglian Injection against HIV-1 in vitro[J];Journal of Henan University of Chinese Medicine;2006-01
8 WANG Xue-bing,CUI Bao-an,WEI Zhan-yong,QIU Jin-li,XU Duan-hong(Engineering College of Animal and Vetrinary Science,Henan Agricultural University,Zhengzhou 450002,China);Studies on Antiviral Effect of Chlorogenic Acid on PRRSV in vitro[J];Journal of Agricultural Science and Technology;2008-03
9 ZHANG Lu;Shanxi Institute of Medicine and Life Science;;Pharmacodynamic Study on New Chinese Veterinary Drug “Huangjinerbai Powder”[J];Animal Husbandry and Feed Science;2014-04
10 LI Chun-hong, TIAN Ji,HE Bing.Institute of Materia Medica of Luzhou Medical College,Sichuan,Luzhou 646000,China;Determination of total flavonoids and total chlorogenic acid in chuan and yu Flos Lonicerae[J];Chinese Journal of Clinical Rational Drug Use;2009-05
China Proceedings of conference Full-text Database 3 Hits
1 FU Yan-ling LIU Ai-ling LI Xu-dong (Ringpu(Tianjin)Bio-Pharmacy Co.,Ltd.Tianjin,300300;China);Advance of the Prevention and Cure of Dairy Cow Mastitis[A];[C];2008
2 ;The Quality Analysis on Wild Honeysuckle in Mi County[A];[C];2013
3 Jianglei 1,zhangyazhong 2 ,mengxiangsong 1, shizhishun 1, ,lijun 1 ,yilei 1 (1bozhou Institute For Food and Drug Control,anhui bozhou 236800;2anhui Institute For Food and Drug Control,anhui hefei 230051);Effect of different processing parameters on the determination of the content of Chrysanthemum morifolium[A];[C];2013
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 WANG Xue-hong et al (Lanzhou Institute of Animal Science and Veterinary Pharmaceutics,The Chinese Academy of Agricultural Sciences,Lanzhou,Gansu 730050);Influence of Hypericum perforatum Extract on IFN-γ,TNF-α of Lung Tissue of Mice Infected with Influenza A Virus[J];Journal of Anhui Agricultural Sciences;2009-13
2 YAN Yinfang, CHEN Xiao, YANG Xiaoqing, et al\ The Institute of Virus Research, The Basic Medical College of Wuhan University, Wuhan 430071\;;A PRELIMINARY STUDY OF THE ACTIVE INGREDIENTS OF VOLATILE OIL OF MOSLA CHINENSIS MAXIM[J];Acta Acadimae Medicinae Qingdao;2002-02
3 LIN CUN-ZHI,WANG JUN,ZHOU ZHAO-SHAN,JIANG HAN-DONG (Department of Respirology,The Affiliated Hospital of Qingdao University Medical College,Qingdao 266003,China);CURATIVE EFFECT OF BUPLEURI DECOCTION JIAWEI ON INFLUENZA A H1N1[J];Acta Academiae Medicinae Qingdao Universitatis;2011-02
4 GUO Shan-shan,HUANG Yang,ZHAO Ye,GAO Ying-jie,GONG Wen-feng,CUI Xiao-lan*(Institute of Chinese Meteria Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China);Effect of Extracted ZG from Gardenia on Hep-2 Cell Membrane Post Infected with Parainfluenza Virus Type 1(PIV-1)[J];Chinese Journal of Virology;2007-05
5 ZHANG Chun-jing1,GU Li-gang2,YU Hai-tao1(1.Department of Biochemistry and Molecular Biology,Department of Cell Biology and Biology Genetics,Qiqihar Medical College,Qiqihar 161042,China;2.Education Ministry Key Laboratory of Anti-virus of Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100029,China);Antagonism of Baicalin on Cell Cyclical Distribution and Cell Apoptosis in A549 Cells Infected with Influenza A(H1N1) Virus[J];Chinese Journal of Virology;2011-02
6 ZHANG Xin,YAN Hui-ping,MA Yin-xue,et al(Research Centre for Infection and Immunity,Beijing You'an Hospital,Beijing 100069);Comparison of rapid antigen(Dot-Elisa) with real time RT-PCR in detection of Flu A[J];Beijing Medical Journal;2010-09
7 YI Shi hong 1,WANG Fang 1,WANG Li ping 2,ZHAO Chun yan *,WEI Qiang 1,LI Fan 1 (1\ Department of Pathogenobiology,School of Basic Medical Sciences,Jilin University, Changchun 130021 China;\ 2\ Department of Pathology,First Hospital,Jilin Univers;Curative effect of Shuanghuanglian injection on anti-virus in vitro[J];Journal of Norman Bethune University of Medical Science;2001-05
8 Liu Lanlin Wang Canhui Yang Jin (Nanjing University of TCM);Constructing Basis and Completing Measures of "Three-Dimensional Syndrome Differentiation Theory" in Exopathic Febrile Diseases[J];China Journal of Traditional Chinese Medicine and Pharmacy;2005-06
9 WANG Le-ping,REN Xiu-ling,GAO Rui-xia(Institution of TCM,Inner Mongolia Medical College,Huhhot 010010,China);Wei qi ying and xue are the basic constructive category of the art of warm disease of TCM[J];China Journal of Traditional Chinese Medicine and Pharmacy;2007-12
10 LU Fang1, WENG Wei-liang1, TIAN Yuan-xiang2 (1Xiyuan Hospital of China Academy of Chinese Medical Sciences, Beijing 100091,China; 2Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, China);Statistical analysis plans optimization of TCM clinical research[J];China Journal of Traditional Chinese Medicine and Pharmacy;2010-08
【Secondary References】
Chinese Journal Full-text Database 3 Hits
1 ZHANG Qing,YANG Bin,WANG Nongrong,DUAN Linjian,HE Shiqin,SUN Jian Fourth Affiliated Hospital of Nanchang University,Nanchang 330003,China;Effect of total flavonoids of Scutellaria baicalensis Georgi on expression of influenza A virus nucleoprotein in HeLa cells[J];Journal of Southern Medical University;2012-07
2 PU Dong;HAO Hong-gang;GUO Jun-hui;Laboratory Department of Kunming Municipal Third People's Hospital;Xian Science/Technology Company;;Application of automated nucleic acid purification system in clinical real-time fluorescence quantitative detection[J];China Tropical Medicine;2013-10
3 LU Nana;LIU Qi;GU Ligang;WU Jun;QIU Zeji;ZHANG Hongchun;CHAO Enxiang;ZHANG Yi;YU Zhuonan;Laboratory of Chinese Medicine on Viral Disease,Basic Medical College,Beijing University of Chinese Medicine;Department of Respiratory Medicine,China-Japan Friendship Hospital;Shanxi University of Traditional Chinese Medicine;;The expression of the inflammation-related cytokines in pneumonia mice infected with influenza virus and regulation of Shufengxuanfei and Jiebiaoqingli herbal anti-virus formulas[J];Chinese Journal of Cellular and Molecular Immunology;2014-03
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 HOU Dong yan 1,HUI Rui hua 1,YANG Mei 2,GUO Hua 1,ZHU Yong qiang 1 (1.Department of Chemistry,Anshan Normal University,Anshan Liaoning 114005,China; 2.Department of Chemistry,Liaoning Normal University,Dalian Liaoning 116029,China);Analysis of chemistry constituents of Lonicera Similis Hemsl on anti-virus chinese traditional medicine[J];Journal of Anshan Normal University;2003-04
2 YAN Yinfang, CHEN Xiao, YANG Xiaoqing, et al\ The Institute of Virus Research, The Basic Medical College of Wuhan University, Wuhan 430071\;;A PRELIMINARY STUDY OF THE ACTIVE INGREDIENTS OF VOLATILE OIL OF MOSLA CHINENSIS MAXIM[J];Acta Acadimae Medicinae Qingdao;2002-02
3 LIU Sheng 2, CHEN Wan Sheng 1, QIAO Chuan Zhuo 1, ZHENG Shui Qing 1, ZENG Ming 1,ZHANG Han Ming 1, SONG Zhao Jun 1 (1. Department of Pharmacognosy, College of Pharmacy, Second Military Medical University, Shanghai 200433, China; 2. Department;Antiviral action of Radix Isatidis and Folium Isatidis from different germplasm against influenza A virus[J];ACADEMIC JOURNAL OF SECOND MILITARY MEDICAL UNIVERSITY;2000-03
4 QU Jing-nian~1,MO Yun-chun~1,LIU Meng-qin~1,LI Rong~2 (1 Department of Chemistry Heng Yang Teacher's College, Hunan Hengyang 421008, China; 2 The Second Hospital Affiliated of Nan Hua University, Hunan Hengyang 421001, China);Extraction of Chlorogenic Acid from Flos Lonicerae by One-step-method and Antimicrobial Abilities of Chlorogenic Acid[J];Huaxue Shijie (Chemical World);2005-03
5 LI Yong mei 1, LI Li 2, BAI Chuan 1, LI Ding 1, WANG Tian zhi 1 1. West China School of Pharmacy,Sichuan University,Chengdu 610041,China; 2. Department of Biology, Fudan University,Shanghai 200437,China;EFFECT OF EXTRACTS FROM HONEYSUCKLE FLOWER ON ANTI-ADENOVIRUS[J];West China Journal of Pharmaceutcal Sciences;2001-05
6 HU Ke jie,SUN Kao xiang,WANG Jing lu, et al (Department of Traditional Chinese Medicine, The Second Affiliated Hospital, Harbin Medical University, Harbin 150086, China);Inhibited effect of Chlorogenic acid on virus in vitro[J];Journal of Harbin Medical University;2001-06
7 Feng Wenyu et al(Pharmacological Reseatrch Department,Luzhou Medical college);ONTRAST STUDY OF THE TECHNIQUES OF EXTRACTION AND PURIFICATIO OF CHLOROGENIC ACID[J];JOURNAL OF LUZHOU MEDICAL COLLEGE;1999-06
8 Dong Jiede Chen Chenhua Qiu Suying Yu Xiuping Zhao Weiming Dept.of Microbiology,Shandong Medical University,Jinan,250012;STUDIES ON THE EFFECTS OF FOUR HERBAL MEDICINES TO COX B_3 AND ECHO 19 VIRUSES[J];;1993-04
9 HU Xing-chang, ZHENG Wei-qiang (Colloge of Life and Environment Sciences, Shanghai Teachers University, Shanghai 200234, China);Resistance to Isatia indigotica Fort Lectin Inhibit Influenza Virus[J];Journal of Shanghai Teachers University;2003-01
10 Chen Shuanglu(Chen SL),Lu Guojin(Lu GJ)(Tianjin Children's Hospital, Tianjin 300074);Study on antibacterial activity in vitro of six antimicrobial drugs compatible with shuanghuanglian injection[J];Chinese Journal of Modern Applied Pharmacy;1998-04
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