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《Chinese Journal of Clinical Hepatology》 2001-04
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The effects of oxymatrine on apoptosis induced by TNF-α in human liver cell line L02

WANG Jun xue,WANG Guo jun,CAI Xiong(Department of Infectious Diseases,ChangZheng Hospital,The Second Military Medical University,Shanghai 200003)  
we investigate the effects of Oxy on apoptosis induced by rhTNF α in human liver cell line L02.Cell viability and proliferation was assayed by trypan blue stain and H 3 TdR co operalion assay.Flowcytometer was used for the cell cycle analysis using propidium iodide DNA binding.Apoptosis can be induced by rhTNF α in L02 cells.Evaluation of the PI DNA binding by flow cytometry showed abolition of G1,S,and G2/M phases and their replacement by a sub 2N AP peak of fragmented DNA.Characteristic apoptic condensations,ranging from single ball condensations to budding,and apoptic bodies were found in cells.Cells treated with Oxy+rhTNF α 15ng/ml partly retain the ability of proliferation,showing no significantly sub 2N Ap peak of fragmented DNA by flow cytometry.Oxy may have inhibitory activity on apoptosis of L02 cells induced by rhTNF α.
【CateGory Index】: R285.5
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