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《Chemistry and Industry of Forest Products》 2007-03
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Determination of Carboxymethyl Cellulase Activity by Glucose Sensor Based on SiO_2 Gel Membrane

GAN Ning1,2, XU Wei-min1, ZHOU Xiong1, WANG Zhi-ying1(1. The State Key Laboratory base of Novel Functional Materials and Preparation Science,Ningbo University, Ningbo 315211, China; 2. Biochemistry Department of Faculty of Life Science,Nanjing Univerisity, Nanjing 210008, China)  
A new biosensor for the assay of carboxymethyl cellulase(CMCE) activity was prepared by modifying a glucose oxidase (GOD) on SiO2 gel film coated on glassy carbon electrode. The amperometric determination was based on electrochemically detected glucose produced by enzymatic reaction of sodium carboxymethyl cellulose(CMC-Na) under the catalysis of CMCE. The modified electrode can sensitively detect CMCE at +0.57 V vs. SCE with a linear response in the range of 5 U/L to 400 U/L under an optimum condition and can be used as a promising method for determination of CMCE activity in micro-analysis.
【Fund】: 中国博士后基金资助项目(2003033483);; 浙江省科技攻关项目(2006C31040);; 浙江省教育厅高校科研计划项目(20061697);; 宁波市自然科学基金资助项目(2006A610048)
【CateGory Index】: O629.8
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