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《Chemistry and Industry of Forest Products》 2015-06
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Screening,Separation and Purification of Mulberry Leaf Polyphenols

ZHOU Hao;LI Wen-jun;ZHANG Jun;ZHANG Yu-si;YAN Xin-pei;WANG Cheng-zhang;The Sericultural Research Institute of Hunan Province;Institute of Chemical Industry of Forest Products,CAF;National Engineering Lab. for Biomass Chemical Utilization;Key and Open Lab. of Forest Chemical Engineering,SFA;Key Lab. of Biomass Energy and Material,Jiangsu Province;  
Ultrasonic-microwave combined technology was used to extract polyphenols from the mulberry leaves and Folin-Ciocalte method was applied to analyze the content of polyphenols in different types and parts of mulberry leaves. The single factor and response surface methodology were successfully used to optimize the extraction conditions. Furthermore,the purification effects of different macroporous resins on the polyphenols were analyzed. The results showed that the highest content of polyphenols was2. 072 % from the type of Damodou,and the polyphenols content of Feng 24 × Miao 33 was the lowest( 1. 035%) among eighteen varieties of mulberry leave. The optimal extraction conditions were 1 g Hu-sang87,liquid to solid ratio 20 ∶ 1( m L ∶ g),the extraction time 93 s,microwave power 326 W,ultrasonic power 304 W. Meanwhile,the yield of crude extracts was 1. 598 % and the purity was 10. 87 %. The most suitable resin for this purification was H103 with adsorbing capacity of 15. 57 mg / g,desorption ratio of 88. 67 % and short adsorption and desorption equilibrium time. The best adsorbing condition of polyphenols sample solution were adsorption column volume 40 m L,loading quantity of 15 g crude extract,mass concentration 3 g / L and the best elution conditions were ethanol volume fraction 60 %,flow rate 2 m L / min,elution volume 80 m L. Then,the purity of polyphenol increased from 10. 87 % to 65. 45 % under these conditions,and in the process of purification,the total yield was 84. 7 %.
【Fund】: “十二五”国家科技支撑计划资助项目(2012BAD36B07)
【CateGory Index】: TQ461
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