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Suitability of weak positive quality control serum for hepatitis B virus DNA detection by polymerase chain reaction

LI Jin ming,DENG Wei,WANG Lu nan, et al. (National Center for Clinical Laboratory)  
To determine the concentration of weak positive quality control serum for hepatitis B virus DNA detection by polymerase chain reaction(PCR).Ten fold series dilution five serum samles which HBV DNA concentrations had been known were distributed to ninty one laboratories of hospitals and reagent manufacturers,and the samples were detected by the routine PCR methods in the laboratories.Then,the data returned were analyzed.At present,the sensitivity of HBV DNA detection by PCR in China is not so high that the sample with 5×10 5 copies/ml HBV DNA has not been detected positive in fifty one(56 0%)of all ninty one laboratories.Moreover,there are great difference in positive rates of five samples detected by laboratories using HBV DNA PCR reagents from different manufacturers.The results suggested that the 10 5 copies/ml HBV DNA is suitable to be as weak positive quality control serum for HBV DNA detection by PCR in China.
【Fund】: 卫生部科研课题!96 0 2 0 42
【CateGory Index】: R512.62
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