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《Journal of Clinical Laboratory Science》 2001-05
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Study on resistance and R plasmid of epidemic strains of Neisseria gonrrhoeae

LI Guo ming,CHEN Qun,WANG Sheng chun(Department of Microbiology,Guangdong Medical College,Zhanjiang 524023,China)  
Objective To investigate the resistance of N.gonorrhoeae epidemic strains to antibiotics and the state of carrying R plasmids.Methods 98 gonococcal epidemic strains obtained from 1998 through 1999 in Zhanjiang(Guangdong)were detected their susceptibilities to ten antibiotics by disk diffusion method,the plasmids were extracted by alkaline lysis technique,and then analysis the distribution of R plasmids.Results The resistance of N.gonorrhoeae to ciprofloxacin,tetracycline,chloramphenicol,penicillin G presented higher level,the resistant percentages were82.65%,69.39%,50%and 32.65%,respectively.Four kinds of different molecular weight plasmids were found,their molecular weight were 42.5 kb,39.5 kb,7.4 kb and 4.2 kb.Among them the presence of 7.4 kb R plasmid was 59.16%.Conclusion The epidemic strains of N.gonorrhoeae in Zhanjiang were resistant to various antibiotics.There were 42 types of resistant profiles;the resistant plasmids were 7.4 kb“Asian type”plasmids.The relationshipas between R plasmids and resistance have a certain characteristic.
【Fund】: 广东省卫生厅“五个一工程”基金 ;;NO .96 0 1;; 广东医学院青年科学基金NO .XQ990 3
【CateGory Index】: R446.5
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