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《Journal of Clinical Laboratory Science》 2002-01
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Detection of the hemolysin activator (eha)Gene of Edwardsiella tarda

GAO Da qing 1,HUAN Xiguan 1,KAN Biao 2 et al. (1 Zhenjiang Medical College,Zhenjing 212001,China 2 Institue of Epidemiology and Microbiology,Chinese Academy of Preventive,China)  
Objective To detect of the hemolysin activator( eha )gene of Edwardsiella tarda and to explore its clinical significance.Methods The eha gene was enhanced by PCR.The probe was labelled with digoxigenin and was used to hybridize in situ and southern blot with 26 strains Edwardsiella tarda .Results New eha gene exited in one copy in some ET chromosomes.Moreover,the phenotype of these Edwardsiella tarda strains was both hemolytic and the nonhemolytic.Conclusion The Edwardsiella tarda strains which has both hemolytic phenomenon and the hemolysin activator( eha )gene were most pathogenic to human.
【Fund】: 江苏省教委基础资金项目 ;( 99KJB310 0 0 7)
【CateGory Index】: R446.5
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