Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Clinical Laboratory Science》 2003-04
Add to Favorite Get Latest Update

Measurement of acid alpha naphthyl acetate esterase in serum by colorimetric assay and its preliminary clinical application

ZHOU yugui,WANG chuanfang,LU Xiaoyun,JIN Yong,LIU Changyuan(Dongtai peoples hospital,Dongtai 224200,Jiangsu Province,China)  
Objective To establish a colorimetric assay for detecting acid alpha naphthyl acetate esterase in serum.Methods When pH was 6.2 and temperature was 37℃,α naphthyl acetate was hydrolyzed by acid alpha naphthyl acetate esterase and it produced α naphthyl.Then colorimetric assay was performed with fast B salt diazotization reaction.Results The optimum pH was 6.0~6.4 and substrate concentration was 2mmol/L.Absorbent wavelength was at 520 nm.Linear range for enzyme determination was from 0 to 2 500 U/L.Time for enzymatic reaction was 20 minutes.Time for production of color reaction was 20 minutes.Time for color stabilization was 20 minutes.Intra assay CV value was 5.1% and inter assay CV value was 2.67%.When Hb concentration rose up to 1g/L and bilirubin to 172μmol/L,enzymatic activity was not interfered.When sodium citrate,sodium oxalate,heparin and EDTA K 2 was common concentration,enzymatic activity was not interfered,but NaF interfered for enzymatic activity.The results did not changed in seven days when sera were stored at 2~8℃ in refrigerator. The mean value in 100 healthy subjects was 1 645±378.5U/L( ±1.96 s ).Conclusions The method was simple and useful.It fit into every laboratory.It should be a new indicator for evaluation of hepatic function.
【CateGory Index】: R446.1
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 2 Hits
1 WANG Yan 1, ZHOU Li 1, ZHANG Yong-hong 1, ZHANG Wei 2, CHEN Yu-mei 1 (1.Department of Digestive Internal Medicine, The Affiliated Hospital of Guiyang Medical College, Guiyang 550004, China; 2.Department of Hematology, The Affiliated Hospital of Guiyang Medical College, Guiyang 550004, China);Measurements of Acid Alpha Naphthyl Acetate Esterase Levels in Serum and Ascites of Liver Cirrhosis Patients[J];Journal of Guiyang Medical College;2004-03
2 PAN Ming-zhi,TAN Ai-juan*,LIU Ai-ying,WANG Jing-jing,HUANG Kai(College of Life Sciences,Guizhou University,Guiyang 550025,Guizhou Province,P.R.China);Optimal liquid medium for producing esterification enzyme by monascus[J];Journal of Mountain Agriculture and Biology;2009-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 HUANG Yi na, CHENG Wei bo, XU Pei yu, YU Wen san, et al. (School of Public Health, Si Chuan University ,Chengdu 610041,China);IMMATURE SD RAT UTEROTROPHIC ASSAY OF BISPHENOL A[J];Carcinogenesis,Teratogenesis and Mutagenesis;2002-04
2 Wang Chunyan Huang Xiuyan(Department of Horticulture,Agronomy College of Shihezi University,Shihezi,Xinjiang 832003);Method of biological slice making adapting to In situ hybridization and In Situ PCR in Plant organization[J];Anhui Agricultural Science Bulletin;2007-07
3 LI Xia et al (College of Life Science,Ludong University,Yantai,Shandong 264025);Microscopic Observation of the Neural Complex of Ascidian Styela clava[J];Journal of Anhui Agricultural Sciences;2009-04
4 HAO Jia sheng 1,\ NIE Qi zhuo 2 (1.Depatrment of Biology, Anhui Normal University,241000, Wuhu, Anhui, China;2.College of Animal Medicine, Nanjing Agricultural University, 210095,Nanjing, Jiangsu, China);STUDIES ON MICROSTRUCTURE AND ULTRASTRUCTURE OF THE SPLEEN OF BUFO GARGARIZANS[J];JOURNAL OF ANHUI NORMAL UNIVERSITY(NATURAL SCIENCE);1999-04
5 YUE Yu-min,DU Jian (The Second People Hospital of Fujian Province, Fujian Fuzhou 350003,China);Effect of Osteoarthritis FormulaⅠ Against Chondrocyte Lesion Induced by Hydrocortisone in Rabbits[J];Journal of Anhui Traditional Chinese Medical College;2005-01
6 JIANG Tao,WANG Chuanfu,LIU Meiguang, et al\ Department of Ophthalmology,The Affiliated Hospital of Qingdao University Medical College, Qingdao 266003\; [;AN EXPERIMENTAL STUDY ON CORNEAL NERVE DAMAGE AND REGENERATION AFTER EXCIMER LASER PHOTOREFRACTIVE KERATECTOMY FOR HIGH MYOPIA[J];ACTA ACADIMAE MEDICINAE QINGDAO;2000-01
7 CHI Jinghua,JI Xiangrui,ZHANG Hua, et al\ Department of Pathology, Qingdao University Medical College, Qingdao 266021[;A STUDY OF THE CONTENT OF DNA AND RNA IN HYDATIDIFORM MOLE AND ITS RELATION WITH MALIGNANT TRANSFORMATION[J];Acta Acadimae Medicinae Qingdao;2001-01
8 Sun Deli,Shen Peizhi,Wang Yuefang, Zhejiang College of Traditional Chinese Medicine,Hangzhou (310009);Effect of Medicinal Vesiculation on Hematopoiesis Function of Rats Treated by CTX Chemotherapy[J];JOURNAL OF ZHEJIANG COLLEGE OF TCM;2000-01
9 LIU Yan-Ling CAI Kai-Hua TANG Hao(Changhai Hospital,The Second Military Medical University Shanghai 200433, China);Establishment of an Animal Model and Improvement of Staining Method for Myocardial Infarction[J];Chinese Journal of Zoology;2002-04
10 GONG Zhi-Jin ZHU Ming-Hua ZHENG Jian-Ming WANG Jian-Jun TAO Wen-Zhao (Department of Pathology, Changhai Hospital, Second Military Medical University, Shanghai 200433,China);Application for a Double-compound Staining Method Showing Nissl Bodies and Medullary Sheath[J];Chinese Journal of Zoology;2004-04
China Proceedings of conference Full-text Database 2 Hits
1 SUN Jing-qiu~1 XU Yan~1 ZHANG Hui-qi~1 YE Jun-qiang~2 (1.College of Life and Environment Sciences,Shanghai Normal University,Shanghai 200234,China; 2.Shanghai Fishery Technical Extension Center,Shanghai 201400,China);Study on Cytochemical Location of Acid Phosphatase and Alkaline Phosphatase of Litopenaeus vannamei[A];[C];2008
2 WANG Shi-feng,WANG Wen-bo (Naval Medical Research Institute,Shanghai 200433,China);The changes of expression of macrophage inflammatory protein—2mRNA in lung tissue of pulmonary oxygen toxicity miceⅠ[A];[C];2011
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 BAO Jia-lan, LI Neng-shu (School of Life Science of Anhui University, Hefei 230039,China);Studies on Ferment Condition of Lipase from Fungi[J];Journal of Anhui University(Natural Sciences);2001-01
2 Tang Bin Zhang Qinqing (Anhui Institute of Mechanical and Electrical Engineering Wuhu 241000);Study of Lipidase Production with Liquid Culture in Monascus Anka[J];JOURNAL OF ANHUI INSTITUTE OF MECHANICAL AND ELECTRICAL ENGINEERING;1997-04
3 Liu Bin et al (Department of Biotechnological and Sciences Hefei University, Hefei 230022);Study on Liquid Fermentation of Rhizopus delemar Lipase and Enzyme Properties[J];Journal of Anhui Agricultural Sciences;2003-06
4 LI Qing-lin,YE Yue-hong,TANG Bo(Department of Clinical Laboratory,Second College of Clinical Medicine,Chongqing University of Medical Sciences,Chongqing 400010,China);Diagnostic value of multiple combined detection indexes in liver diseases[J];Chongqing Medicine;2007-10
5 XIAO Jian-Jun HUA Ze-Zhao CHEN Er-Tong WANG Zhong-Hai ZHAO Xiao-Lei (Institute of Refrigeration and Cryogenics, Shanghai University of Science and Technology, Shanghai 200093, China);THE STUDY OF INFLUENCE OF REFRIGERATION AND CRYOPRESERVATION ON THE ENZYME ACTIVITY OF WHEAT ESTERASE[J];Journal of Engineering Thermophysics;2003-04
6 HU Zhaoyang,WEI Hanning,LI Chunyuan,WU Bo (College of Life Science and Technology,Guangxi University,Nanning 530005,China);Screening of lipase producing strains and studing on properties of lipase[J];Journal of Guangxi Agricultural and Biological Science;2006-03
7 Jin Qirong, Xu Ganrong, Zhou Yisheng(Department of Fermentation Technology, Wuxi Institute of Light Industry, Wuxi);Production and Properties of Thermostable Lipase by Rhizopus sp.[J];Industrial Microbiology;1995-01
8 XU Yan, XIE Hong xiang 1, WANG Dong, ZHAO Guang ao (School of Biotechnology,Wuxi University of Light Industry,Wuxi 214036) SUN Yu fa, FEI Guo hua (Yangshao Distillery, Mianci 472000);Fermentation Conditions of Lipase Production by Rhizopus Y 92[J];INDUSTRIAL MICROBIOLOGY;1999-01
9 Tan Aijuan Liang Zongqi Liu Aiying (College of Chemical and Bi-Pharmacy ,Guizhou University ,Guiyang 550025,China; College of Bioscieme and Environmental Science ,Guizhou University, Guiyang 550025, China);Orthogonal Experiment on the Optimum Culture Conditions of Paecilom yces cicadae[J];Journal of Huazhong Agricultural;2004-01
10 LUO Chuanyi, SHI Jingrong, DAI Chuanbo (Jilin Institute of Chemical Technology, Jilin 132022);Program for Orthogonal Design Variance Analysis Based on Excel[J];Computer Engineering;2002-11
【Secondary References】
Chinese Journal Full-text Database 2 Hits
1 Chen Zhiming,Liu Xingxiang(Huaian Municipal Fourth People′ s Hospital,Huaian,Jiangsu 223002,China);Clinical value of FibroScan meter in diagnosing hepatic fibrosis in viral hepatitis[J];Chongqing Medicine;2011-13
2 YANG Xiao-jun1,2,YANG Cheng-long1,HE Zhi-gang1,LU Dong-he1(1.Ago-engineering Technique Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou, Fujian 350003,China;2.College of Food Science,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China);Advances in studies of monacsus submerged culture[J];Fujian Journal of Agricultural Sciences;2010-06
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved