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Measurement of acid alpha naphthyl acetate esterase in serum by colorimetric assay and its preliminary clinical application

ZHOU yugui,WANG chuanfang,LU Xiaoyun,JIN Yong,LIU Changyuan(Dongtai peoples hospital,Dongtai 224200,Jiangsu Province,China)  
Objective To establish a colorimetric assay for detecting acid alpha naphthyl acetate esterase in serum.Methods When pH was 6.2 and temperature was 37℃,α naphthyl acetate was hydrolyzed by acid alpha naphthyl acetate esterase and it produced α naphthyl.Then colorimetric assay was performed with fast B salt diazotization reaction.Results The optimum pH was 6.0~6.4 and substrate concentration was 2mmol/L.Absorbent wavelength was at 520 nm.Linear range for enzyme determination was from 0 to 2 500 U/L.Time for enzymatic reaction was 20 minutes.Time for production of color reaction was 20 minutes.Time for color stabilization was 20 minutes.Intra assay CV value was 5.1% and inter assay CV value was 2.67%.When Hb concentration rose up to 1g/L and bilirubin to 172μmol/L,enzymatic activity was not interfered.When sodium citrate,sodium oxalate,heparin and EDTA K 2 was common concentration,enzymatic activity was not interfered,but NaF interfered for enzymatic activity.The results did not changed in seven days when sera were stored at 2~8℃ in refrigerator. The mean value in 100 healthy subjects was 1 645±378.5U/L( ±1.96 s ).Conclusions The method was simple and useful.It fit into every laboratory.It should be a new indicator for evaluation of hepatic function.
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