Detection of reticulocytes counts and its subpopulation in clinical application
YUE Jiaxin,CONG Yulong,LAN Yating, et al. (Department of Clinical Laboratory in PLA General Hospital,Beijing,100853,China)
Objective To establish reference values of the reticulocytes counts and its subpopulation in healthy adults and evaluate its importance in clinical applications.Methods The reticulocytes were counted by automated hematology analyzer (Advia 120) in 655 healthy adults and 71 patients with hematological disorders.Results Student′s t test showed that there were ststistically significant differences( P 0.05)between male and female for reticulocyte percentage (RET) and reticulocyte absolute number (RET#).RET and RET# were 0.0145±0.0043 and(71.86±22.71)×10 9/L for male,0.012 2±0.004 1 and(55.36±20.23)×10 9/L for female,rspectively. High fluorescence reticulocytes (HFR),middle fluorescence reticulocytes (MFR) and low fluorescence reticulocytes (LFR) were 0.020 8±0.013 9,0.095 6±0.032 1 and 0.8836±0.038 4 in male and female.In hemolytic anemia patients (18 cases) both the RET# and HFR greatly increased. RET# greatly and HFR moderately decreased in leukemia patients after chemotherapy (20 cases).In IDA (iron deficiency anemia) patients (13 cases) RET# did not differ from the healthy adults but HFR slightly increased before therapy, RET# and HFR moderately increased one week after therapy.In aplastic anemia patients (7 cases) RET# decreased and HFR increased moderately.Conclusions Reference value of reticulocyte percentage (RET) and reticulocyte absolute value (RET#) were provided separately according to gender in healthy adults.Reticulocyte subpopulation was not correlated with gender.There were statistically significant differences( P 0.01)between the hematological disorders and healthy adults for RET# and HFR.