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《Journal of Clinical Laboratory Science》 2005-03
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Genotyping of Acinetobacter baumannii by randomly amplified polymorphic DNA technique

YE Mingliang,WU Haihuan,HOU Xiaoqi,LU Bo(Jinan Military General Hospital,Jinan 250031,China)  
Objective To establish a genotype spectrum of Acinetobacter baumannii (A.baumannii) by randomly amplified polymorphic DNA (RAPD) technique.Methods The arbitrary primer and reaction system of RAPD were optimized for genotyping 176 strains of A.baumannii isolated from clinical samples and the fingerprinting spectrum was drawn according to the number of bands and length of DNA fragments.Results Among the 176 strains of A.baumannii 122 kinds of RAPD fingerprinting were established,in which the type 68,58 and 66 were the major types in the author's hospital.Conclusions In 176 strains of A.baumannii isolated from clinical samples 122 kinds of fingerprinting could be obtained by RAPD technique.The type 68,58 and 66 were the major epidemic types in the author's hospital.
【Fund】: 军队医药卫生基金课题(98Q012)。
【CateGory Index】: R446.5
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