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《Journal of Clinical Stomatology》 2004-07
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Experimental study on the apoptosis of vascular endothelial cells induced by arecoline

YIN Xiao-min, PENG Jie-ying, FANG Chang-yun , GAO Yi-jun,PANG Dan-lin,LI Ming.Department of Stomatologe,Xiangya Hospital, Central South University , Changsha 410078,China  
Objective: To investigate the apoptotic effect of arecoline on the cultured human umbilical vein endothelial cells(HUVECs).Method: After the VEC exposed to the increasing concentrations of arecoline (30-150 ug/ml). Apoptotic morphological changes and percentage of apoptosis were assayed with Hoechst 33258 staining. DNA ladder was observed!by agarose gel electrophoresis. Result:①The obvious morphological alterations of apoptosis could be observed under the fluorescence microscopy and percentage of apoptosis increased in a time and dose dependant manner. The percentage of apoptosis was significantly higher in the arecoline treated group than that in normal control group( P 0.01). ②In VEC group treated by 90 ug/ml arecoline, characteristic DNA “ladder" was seen by agarose gel electrophoresis . Conclusion: Arecoline induced the apoptosis of VEC in a dose and time dependant manner.The apoptosis of VEC induced by arecoline may contribute to the development and progression of Oral Submucous Fibrosis (OSF).
【Fund】: 湖南省科技厅发展基金资助 (0 2SSY30 52 )
【CateGory Index】: R285
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【References】
Chinese Journal Full-text Database 5 Hits
1 YIN Xiao-min,PENG Jie-ying,GAO Yi-jun,FANG Chang-yun,PANG Dan-lin,XIA Yu.Department of Stomatology,Xiangya Hospital,Central South University,Changsha 410078,China;The effects of Salvia Miltiorrhizae on the apoptosis of vascular endothelial cells induced by arecoline[J];Journal of Clinical Stomatology;2005-10
2 Huang Yulin,Wang Ming,Zhang Xinying,Zhang Haide,He Jiqin (College of Technology,South China University of Tropical Agriculture,Danzhou,Hainan 571737,China);Advances of Research on Bioactivity Substances of Areca Fruit[J];Academic Periodical of Farm Products Processing;2007-07
3 WANG Guang,HU Bi(Department of Physiology, School of Medicine, University of South China, Hengyang Hunan 421001, China);Research progress of arecoline[J];International Journal of Pathology and Clinical Medicine;2010-02
4 YIN Xiao-min1, PENG Jie-ying1, Gao Yi-jun2, FANG Chang-yun1, PANG Dan-lin1, XIA Yu1 (1.Department of Stamotology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R.China; 2.The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R.China);Effect of salvia miltiorrhizae injection on apoptosis of endothelia cells induced by arecoline and the aetivity of caspase-3[J];China Journal of Modern Medicine;2005-09
5 LI Ming1,2,PENG Jieying2,WU Yingfang2,LI Jijia2,CAO Qing3(1.Changsha Stomatological Hospital,Changsha 410005;2.Department of Stomatology,Xiangya Hospital, Central South University,Changsha 410078;3.Department of Oncology,Xiangya Hospital, Central South University,Changsha 410078,China);Apoptosis of Hacat cells induced by arecoline[J];International Journal of Pathology and Clinical Medicine;2011-04
【Citations】
Chinese Journal Full-text Database 2 Hits
1 X Chunjiao, PENG Xieying , LIU Shufan , et al Department of Stomatology, Xiangya Hospital, Hunan Medical University, Changsha 410008 ,China;Localization of endothelin-1 in biopsies from oral submucous fibrosis: immunoelectron microscopic study[J];CHINESE JOURNAL OF STOMATOLOGY;2000-03
2 Gao Yijun, Ling Tianyou, Wu Hanjiang, et al. Department of Stomatology, the Second Affiliated Hospital, Hunan Medical University, Changsha 410011;Expression of transforming growth factor β 1 in keratinocytes of oral submucous fibrosis tissue[J];CHINESE JOURNAL OF STOMATOLOGY;1997-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 WU Xiang-qing,LING Tian-you,LI Xia,et al.Department of Oral and Maxillofacial Surgery.The Second Xiangya Hospital,Central South University,Changsha,410011,China [;Expression of the Endothelin-1 Receptors in Oral Submucous Fibrosis[J];Journal of Chinese Physician;2003-08
2 WU Yingfang1,PENG Jieying2,QUE Guoying1,XU Chunjiao2,YIN Xiaomin1 (1.Department of Stomatology,Xiangya Hospital,Central South University,Changsha 410008;2.Department of Periodontology and Oral Medicine,College of Stomatology,Central South University,Changsha 410078,China);Therapeutic effect of the combination of traditional Chinese medicine and western medicine on patients with oral submucous fibrosis[J];Journal of Central South University(Medical Science);2010-04
3 Feng Yunzhi, Ling Tianyou\;Department of Stomatology, the Second Affiliated Hospital, Hunan Medical University;Changes of Cytokines Secreted by Human Oral Mucosa Keratinocytes from Oral Submucous Fibrosis in vitro[J];WEST CHINA JOURNAL FOF STOMATOLOGY;2000-01
4 Xu Chunjiao, Peng Xieying, Liu Shufan, et al\;Department of Stomatology, Xiangya Hospital, Hunan Medical University\;;Quantitative and Immunohistochemical Analysis of Endothelin-1 in Oral Submucous Fibrosis[J];West China Journal of Stomatology;2000-06
5 Feng Yunzhi, Ling Tianyou\;Department of Stomatology, the Second Xiangya Hospital, Central South University\;;Effects of Areca Nut Extract on Levels of Intercellular Adhesion and Expression of Oral Fibroblasts[J];West China Journal of Stomatology;2002-04
6 LI Ning1,JIAN Xin-chun1,XU Chun-jiao2. (1. Dept. of Oral and Maxillofacial Surgery, Xiangya Hospital of Central South University, Changsha 410008, China; 2. Dept. of Oral Medicine, Xiangya Hospital of Central South University, Changsha 410008, China);Expression of loricrin and cytochrome P450 3A5 in oral submucous fibrosis and their significance[J];West China Journal of Stomatology;2009-01
7 LIU Zhi-wen,CHEN Jian-sheng,LI Lin,GONG Bo-tao,LING Tian-you.(Department of Stomatology,Hospital of Traditional Chinese Medicine of Zhongshan,Zhongshan 528400,China);Study on expression of Smad7 protein in oral submucous fibrosis[J];Stomatology;2006-06
8 GAO Yi-jun,LING Tian-you,YIN Xiao-min,et al.Department of Stomatology,The Second Xiangya Hospital,Central South University,Changsha 410011;Experimental Study on the Apoptosis of Oral Keratinocytes Induced by Arecoline[J];Journal of Oral Science Research;2007-06
9 PENG Jie-ying,XING Quan,WANG Jing,et al.Department of Stomatology,Xiangya Hospital,Central South University,Changsha 410008;Expression of ETA and ETB mRNA in Oral Submucous Fibrosis[J];Journal of Oral Science Research;2008-01
10 LI Xia,LING Tian-you.Stomatology Department of the Second Hospital of Xiangya Medical School,Centre South University.Changsha 410011,China;The effect of aqueous areca nut extract on the level of NO produced by endothelial cells[J];Journal of Clinical Stomatology;2004-08
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Hu Yixiu, Zang Xuebing, Qiu Feng, Nie Yan, Hu Yuming, Hu Shumei, Pan Changhuai, Zhou Yuechan Hunan Health and Epidemic Prevention Station, Changsha\ 410005;THE EFFECT OF ARECA NUT ON THE SPERMS OF MICE[J];CARCINOGENESIS, TERATOGENESIS AND MUTAGENESIS;1999-01
2 XU Hong 1* ,SHI Xiao-ping 2 ,TANG Wei-ping 2 ,LI Li-xiang 3 1.Department of Otorhinolaryngolo gy,2.Department of stomatology,3.Department of Pathology,The Seco nd Affiliate Hospital of Jiang-Xi Medical College,Nan-Chang 330006,P.R;Expression and Clinical Significan ce of Matrix Metalloproteinase-9an d Vascular Endothelial Growth Factor in Oral Squamous Cell Carcinomas[J];Chinese Journal of Cancer;2002-09
3 WU Jiang-Xue, XU Ben-Ling, HUANG Wen-LinResearch Department, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong, 510060,P.R.China;Research Advancement of Endogenous Angiogenesis Inhibitors[J];Chinese Journal of Cancer;2005-03
4 Ye Qing,Chen Peifeng Zhejiang Chinese Medical University,Hangzhou(310053);Review on Tumor Vessel Production Inhibited by Chinese Drugs Extract[J];Journal of Zhejiang University of Traditional Chinese Medicine;2007-04
5 ZHAO Tian-Fu~(①②) LI Jing~①(①State Key Laboratory of Reproductive Biology,Institute of Zoology,Chinese Academy of Sciences,Beijing 100080; ② Department of Biology,Baoding Teachers' College,Baoding 071000,China);Molecular Mechanisms of Angiostatin Action: New Advances[J];Chinese Journal of Zoology;2006-03
6 HUANG Bing Bing 1, FAN Ming Wen 1, YANG Xiang Liang 2, HAN Ding Xian 2 1Department of Operative Dentistry, Cariology and Endodontology, Stomatological College, Wuhan University, Wuhan 430079, China, 2Institute Pharmaceutical, Huazhong University of Science and Technology, Wuhan 430074, China;Effects of Chinese traditional herbs on growth of periodontopathic bacteria[J];Journal of The Fourth Military Medical University;2003-05
7 LU Di 1 , BAI Xiao-chun 1 , LIU Bin 2 , LI Xiu-mei 1 , DENG Fan 1 , LI Ming 1 , CHENG Bao-luan 1 , LUO Shen-qiu 1 Department of Cell Biology 1 , Department of Orthopedic and Spinal Surgery, Nanfang Hospital 2 , First Military Medical University, Guangzhou 510515, China;Hydrogen peroxide inhibits arsenic trioxide-induced apoptosis of Burkitt lymphoma cells[J];Journal of First Military Medical University;2004-04
8 ZHOU Xu-zheng,ZHANG Ji-yu,ZHAO Cheng-ying,LI Jin-shan,ZHAN Zhan-feng,LI Jian-yong,WEI Xiao-juan,NIU Jian-rong,WANG Ling(Key Laboratory of New Animal Medicine Project,Ministry of Agricultrure,Lanzhou Institute of Animal and Veterinary Pharmceutical Sciences,Chinese Academay of Agricultral Sciences,Lanzhou,Gansu,730050,China);Acute Toxicity of Arecoline Hydrobromide in Mice and Rat in Taking Orally[J];Progress in Veterinary Medicine;2007-09
9 Du Zhimin.Wan Xinxiang, Wu Aichan,Chen Yiyue (Guangzhou Medical College, PLA. Guangzhou, 510315);Effect of Arecoline on spontaneous peristalsis of isolated ileum[J];;1998-02
10 Du Zhimin, Wan Xinxiang,Wu Aichan, Chen Yiyue (Guangzhou Junior Medical College, PLA Guangzhou, 510315);Effect of Arecoline on movement of small intestine in mice[J];;1999-01
【Secondary References】
Chinese Journal Full-text Database 4 Hits
1 TANG Li1,2,DENG Li2*(1.West China School of Pharmacology,Sichuan University,Chengdu 610041,China;2.Division of Stem Cell and Tissue Engineering,State Key Laboratory of Biotherapy,West China Hospital,Sichuan University,Chengdu 610041,China);Protective Effect of Danshen Injection against Hopoxia Injury in Human Umbilical Vein Endothelial Cells[J];Lishizhen Medicine and Materia Medica Research;2011-05
2 Wang Wei, Chen Qingxi (College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002);Progresses on Fruit Active Ingredient and Anti-oxidation[J];Chinese Agricultural Science Bulletin;2010-06
3 YANG Fuguo1, CHEN Zuoyuan2, LIAN Zhexun2, GUO Yunliang3, LIU Gexin1, DONG Guoxiong2 (1.Department of Medicine, Nursing School of Qingdao University Medical College, Qingdao, Shandong 266021, P.R.China; 2.Department of Vascucardiology, 3.Institute of Cerebrovascular Diseases, the Affiliated Hospital of Qingdao University Medical College, Qingdao, Shandong 266003, P.R.China);Inhibitory effects of salvianolic acid B on myocardial cellular nuclear transfer of NF-κB p65 and the expression of TNF-α during ischemia-reperfusion in rabbits hearts in vivo[J];China Journal of Modern Medicine;2009-05
4 LI Ming1,2,PENG Jieying2,WU Yingfang2,LI Jijia2,CAO Qing3(1.Changsha Stomatological Hospital,Changsha 410005;2.Department of Stomatology,Xiangya Hospital, Central South University,Changsha 410078;3.Department of Oncology,Xiangya Hospital, Central South University,Changsha 410078,China);Apoptosis of Hacat cells induced by arecoline[J];International Journal of Pathology and Clinical Medicine;2011-04
【Secondary Citations】
Chinese Journal Full-text Database 6 Hits
1 REN Ming, ZENG Zhi, LIU Xiao jing, et al The First Affiliated Hospital, West China University of Medical Science (Chengdu,610041,China);Content of TGF-β_1 and Type TV Collagen in Serum of Coronary Heart Disease Patients and the Relationship with Vascular Endothelial Cell Injury[J];Journal of Chengdu Unversity of Tarditional Chinese Medicine;2001-03
2 Jian Xinchun;Liu Shufan;Shen Zihua,Department of Stomatology,Affiliated First Hospital of Hunan Medical CollegeYang Yuanhua,Department of Pathology,Hunan Medical College;Histomorphologic Observation on Epithelial Tissue of 24 Cases of Oral Submucous Fibrosis[J];West China Journal of Stomatology;1988-04
3 Gao Yijun; Ling Tianyou; Wu Hanjiang;et al (Department of Stomatolgy, the Second Affiliated Hospital,Hunan Medical University);The mRNA Expression of Transforming Growth Factorβ_1 in Oral Submucous Fibrosis Tissues──In Situ Hybridization Study[J];WEST CHINA JOURNAL FOF STOMATOLOGY;1996-01
4 Xu Chunjiao, et al. Department of Stomatology, Hunan Medical University,Changsha 410078;The Expression of Endothelin 1 (ET 1) in Oral Submucous Fibrosis[J];JOURNAL OF CLINICAL STOMATOLOGY;1999-01
5 X Chunjiao, PENG Xieying , LIU Shufan , et al Department of Stomatology, Xiangya Hospital, Hunan Medical University, Changsha 410008 ,China;Localization of endothelin-1 in biopsies from oral submucous fibrosis: immunoelectron microscopic study[J];CHINESE JOURNAL OF STOMATOLOGY;2000-03
6 YAN Guofu, HE Wei, LIU Rongqing, et al. Department of Dermatology,Xinqiao Hospital, Third Military Medical University,Chongqing 400037;Effect of Vascular Endothelial Cells on Fibroblast Proliferation[J];CHINESE JOURNAL OF DERMATOLOGY;2000-01
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