Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Clinical Internal Medicine》 2008-01
Add to Favorite Get Latest Update

Expression of peroxisome proliferator-activated receptor γ in human colon cancer and growth inhibition by its agonists

SHEN Dan,DENG Changsheng.Department 1 of Internal Medicine & Geriatrics,Zhongnan Hospital,Wuhan University Wuhan 430071,China  
Objective To investigate the expression of peroxisome proliferator-activated receptor γ (PPARγ) in human colon cancer, as well as the pathway and mechanism of PPARγ agonists inhibiting colon cancer cell growth.Methods The expressions of PPARγ in 56 colon cancer tissues and normal colonic tissues were detected by immunohistochemical methods.The expressions of PPARγ in SW480 and LS174T colon cancer cells were detected by RT-PCR and Western-blot analysis.MTT colorimetric assay was used to detect the inhibition rate.Flow cytometry was used to detect the cell cycle and apoptosis.Results PPARγ was over-expressed in colon cancer tissues (71.4%) than in normal colonic tissues (44.6%) (P0.01).Assessed by Dukes stage, the PPARγ expressions in C+D stages (82.9%) were significantly higher than those in A+B stages (52.4%) (P0.05).The PPARγ expressions in cases with lymph node or liver metastasis (81.1% or 100%,respectively) were higher than those without lymph node or liver metastasis (52.6% or 66.0%,respectively) (P0.05).Treatment with PPARγ agonists,15-deoxy-12,14 -prostaglandin J2 (15d-PGJ2) or Pioglitazone (PGZ),inhibited the growth of SW480 and LS174T cells in a time-and dose-dependent manner.The growth inhibitory effect of PPARγ agonists could only be partly reversed by PPARγ antagonist GW9662.Flow cytometry was performed.It demonstrated that a large portion of SW480 and LS174T cells was arrested at G0/G1 phase,and the apoptotic rate of two colon cancer cells was significantly increased.Conclusions PPARγ can be used as an important predictor involved in carcinogenesis and development of colon carcinoma.15d-PGJ2 and PGZ may suppress colon cancer cells partly in PPAR-dependent manner, and this inhibitory effect may be associated with the induction of cell differentiation and apoptosis.
【CateGory Index】: R735.35
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved