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《The Journal of Clinical Anesthesiology》 2007-09
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Effects of isoflurane on synthesis of phosphatidylcholine in adult rat lungs

AN Hai-yan,YANG Ba-xian,LI Zhi-hong,et al.Department of Anesthesiology,Peking University People's Hospital,Beijing 100044,China  
Objective To investigate whether inhaled different concentrations of isoflurane(Iso)may affect the synthesis of phosphatidylcholine(PC)in mature lungs.Methods Seventy-eight adult male Wistar rats were randomly divided into four groups.Furthermore,for each of group A to D,3 subgroups(n=6)were established according to the duration of the gas administration.High performance liquid chromatography(HPLC)was employed to deter mine the PC concentration in bronchoalveolar lavage fluid(BALF).The mRNA expression and protein of cytidine triphosphate:phosphocholine cytidylyltransferase(CCT)were measured by reverse transcription polymerase chain reaction(RT-PCR)and Western blot.Results The level of mRNA and protein of CCT in lung homogenates were not significantly different among all groups.Compared with group E and 4 h subgroup,in group B2 and C2,both the PC concentration in BALF and the expression of microsomal CCT in pulmonary tissue reached the nadir(P0.05)and recovered at 2 hours after the cessation.During 8 h exposure,in group B2 and C2,the PC concentration and the expression of microsomal CCT were lower than in group D2(P0.05),while both parameters in group C2 were also lower than in group A2(P0.05).Conclusion Isoflurane(1.5%,2.0%)inhalation for a longer duration(8 hours)may reversibly impair the de novo phospholipids synthesis in rat lung,of which the mechanism may be associated with the decreased activity of CCT.
【Fund】: 教育部高等学校博士学科点专项科研基金(B661)
【CateGory Index】: R96
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【Citations】
Chinese Journal Full-text Database 2 Hits
1 GONG Xiaohui, HU Xiaowei, SUN Bo. Laboratory of Pediatric Respiratory and critical Care Medicine, Children's Hospital of Fudan University, Shanghai 200032, China;Effects of inhaled nitric oxide on denovo synthesis of phosphatidylcholine in adult rat lungs with endotoxin-induced inflammatory injury[J];Journal of Emergency Medicine;2004-06
2 Sun ying,Hang Yannan,An Xiaohu,et al. Department of Anesthesiology,Children's Medical Center Xinhua Hospital SSMU,Shanghai 200127 CHINA;The effects of inhalation of volatile anesthetics with nitric oxide on lung mechanics and pulmonary surfactant in healthy piglets[J];The Journal of Clinical Anaesthesiology;2004-09
【Co-citations】
Chinese Journal Full-text Database 3 Hits
1 SHANG GUAN Wang-ning LIU Jin Department of Anesthesiology,West China Hos-pital,SiChuan University,Chengdu 610041 ,China;The effects of inhaled anesthetics on lung[J];International Journal of Anesthesiology and Resuscitation;2007-04
2 ZHU Yourong, GONG Xiaohui, LV Yong, et al.Laboratory of Pediatric Respiratory and Intensive Care Medicine, Childrens Hospital, Fudan University,Shanghai 200032, China;Surfactant and inhaled nitric oxide in treatmont of acute lung injury induced by escherichia coli peritonitis in pigs[J];Journal of Emergency Medicine;2004-10
3 Jiang Ming,Gu Xiaoping,Ma Zhengliang.Department of Anesthesiology,Affiliated Drum Tower Hospital,Medical School of Nanjing University,Nanjing 210008,China;Effects of Sevoflurane and Isoflurane on Ventilation Function during Laparoscopic Cholecystectomy[J];Chinese Journal of Minimally Invasive Surgery;2009-08
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Guo Zhenghui,Hong Xin,Mao Baoling,et al.Department of Instisute of Respiratory Disease,Xingqiao Hospital,Third Military Medical University,Chongqing 400037,China [;Effects of nuclear factor-κB activation on acute lung injury[J];Journal of Emergency Medicine;2003-09
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