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《Journal of Clinical Anesthesiology》 2017-10
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Establishing mouse model of type 2 diabetes peripheral neuropathy and measuring its intra-epidermal nerve fiber density

PU Jiangpan;TANG Jiawen;ZHU Tao;Shanghai Songjiang district center hospital affiliated to Nanjing Medical University;  
Objective To establish mouse model of type 2 diabetes peripheral neuropathy and measure its intra-epidermal nerve fiber density(IENFD).Methods Male C57BL6 mouse were randomly divided into four groups:group HS(n=6):high-fat diet+single streptozotocin intraperitoneal injection(120 mg/kg);group H(n=6):high fat diet+buffer injection;group S(n=6):standard chow diet+single streptozotocin intraperitoneal injection(120 mg/kg);group C(n=6):standard chow diet+buffer injection.The 24 th week was the end point of the experiment,and random glucose,homeostasis model assessment of insulin resistance(HOMA-IR),mechanical threshold,and IENFD were measured.Results Group HS had significantly higher random glucose and HOMA-IR than other groups(P0.01),had significantly lower mechanical threshold than other groups(P0.05),had significantly lower IENFD than groups S and C at 24 th week(P0.05);group H had significantly higher random glucose and HOMA-IR than group C at 24 th week(P0.01),had no significant difference in mechanical threshold compared with group S and group C,and had no significant difference in IENFD compared with group HS.Conclusion A mouse model of type 2 diabetes peripheral neuropathy was successfully established,and the IENFD was found to be decreased significantly.
【Fund】: 上海市科委医学引导项目基金资助(134119b2700)
【CateGory Index】: R-332;R587.2
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