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《Journal of Clinical Dermatology》 2003-02
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Rapid detection and identification of Candida species with ITS universal primers by PCR

WANG Ying,GU Jun,LIU W ei-da(Department of Dermatology,Changhai Hospital,The Second Military Medic al University,Shanghai200433,Chi na)  
Objective:To set up a rapid and simple PCR assay for Candida detection and identification by using in ternal tran scribed spacer(ITS)universal primers.Methods:Fungal suspensions of7species(8candidal strains)were am pli fied by using two pairs of ITS uni versal primers ITS1,ITS4and ITS86,ITS4.Results:The species-specific DN A bands of7species of Candida in the fungal suspensions could be amplified with two pairs of ITS universal primers within3.5hours.Moreover,the second pai r was more spesies-specific.Conclusions:ITS universal primers combined with P CR assay can detect Can dida rapidly,simply,specifically and sensitively,even can identify some species at the same time.The assay will have potential val ue in the early diagnosis and treatment of deep fungal infections.
【CateGory Index】: R446.5
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