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《Journal of Clinical Dermatology》 2008-03
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Effect of hesperidin on B16 and HaCaT cell lines irradiated by narrowband-UVB light

ZHANG Ru-zhi, ZHU Wen-yuan, XIE Fang, GE Xiao-song, ZHAO Hao, JIN Hui-ling (Department of Dermatology, the Affiliated Hospital of Bengbu Medical College, Bengbu 233004, China)  
Objective: To investigate the effects of hesperidine on B16 and HaCat cell lines irradiated by Narrowband-UVB light. Methods: MTT, NaOH assay, Takahashi technique were applied to measure the proliferation of B16 and HaCaT cells, the synthesis of melanin, and the tyrosinase activity of B16 cell respectively. The expression of CXC receptor 2 (CXCR2) on keratinocytes was analyzed by flow cytometry. To detect the contents of transforming growth factor-β1 (TGF-β1), enzyme linked immunosorbent assay was applied. Results: The findings indicated that hesperidine at the concentrations of 50-600 mg/L did not affect the proliferation of B16 and HaCaT cells. At the doses of 200 mg/L and over, hesperidine inhibited the tyrosinase activity and melanogenesis in B16 cells. The levels of TGF-β1 in the supernatant of cultured keratinocytes presented as "wave shape" according to hesperidine contents. The expression of CXCR2 in the normal cultured keratinocytes did not change with different concentrations of hesperidine. However, the expression of CXCR2 in the cultured cells irradiated by narrowband-UVB light was increased obviously. When 200-600 mg/L hesperidine were added to the media, in which the cultured keatinocytes would be irradiated by Narrowband-UVB light later, the expression of CXCR2 decreased markedly in a dose-dependent manner, especially, when the dosage of hesperidine was 600 mg/L. Conclusions: Hesperidine has various biological effects, with gentle effectiveness, and without cytotoxicity. It is a potent plant extract for whitening and photoprotection.
【Fund】: 安徽省教育厅科研基金资助项目(2005kj048zd);; 2006宝洁美尚科技基金资助项目
【CateGory Index】: R285.5
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【Citations】
Chinese Journal Full-text Database 2 Hits
1 YUE Xue-zhuang, ZHU Wen-yuan, MA Hui-jun, WANG Da-guang, LI Cheng-rang (Department of Dermatology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China);Effect of topical application of hesperidin, aescin and tanshinone on finger nailfold skin microcirculation[J];Journal of Clinical Dermatology;2005-07
2 ZHANG Ru-zhi, ZHU Wen-yuan;Development of skin depigmenting agents[J];Journal of Clinical Dermatology;2006-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 SHI De-fang et al (National Research and Development Center for Edible Fungi Processing,Agricultural Products Processing Subcenter of Hubei Agricultural Science and Technology Innovation Center,Research Institute of Agricultural Products Processing and Nuclear-Agricultural Technology,Hubei Academy of Agricultural Sciences,Wuhan,Hubei 430064);Research Advances on Physiological Activity Function of Hesperidin and Its Extraction Technique[J];Journal of Anhui Agricultural Sciences;2008-10
2 ZHANG Sheng-ming (Department of Chemistry,Qinghai Nationalities University,Xining,Qinghai 810007);Study on the Extraction of Polysaccharide from Rhodiola crenulata by Alkali Method and Its Antioxidation[J];Journal of Anhui Agricultural Sciences;2008-18
3 XUE Dong-zhang1,ZHANG Ru-zhi2,JIANG Cong-jun2,ZHU Wen-yuan3(1.Department of Pharmacology,Bengbu Medical College,Bengbu Anhui 233030;2.Department of Dermatology,The First Affiliated Hospital of Bengbu Medical College,Bengbu Anhui 233004;3.Department of Dermatology,The First Affiliated Hospital of Nanjing Medical University,Nanjing Jiangsu 210029,China);The influence of bacteria motif on melanocytes and in prostaglandim E_2[J];Journal of Bengbu Medical College;2008-06
4 XIANG Guang-ya, HAO Qiao-ling, YUAN Jin-wei, ZHOU Yi-kai (Institute of Enviromental Medicine of Tongji Medical College, Central China University of Sciences and Technologies, Wuhan, Hubei 430030);Studies on Effect of Hypericum Chinense on Eliminating Active Oxy-radical[J];Academic Journal of Guangdong College of Pharmacy;2000-04
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【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 LOU Gui yu 1, JIANG Yu 1, PENG Jia he 1, QIAN Min zhang 2 ( 1Department of Biochemistry, College of Basic Medicine, Third Military Medical University, Chongqing 400038; 2Department of Biochemistry, Zunyi Medical College, Zunyi 563003, Guizhou Province, China);Effects of hesperidin against lipid-oxidation and its effects on transcription of MCP-1[J];Acta Academiae Medicinae Militaris Tertiae;2004-08
2 YUE Xue-zuang, ZHU Wen-yuan;The color of the skin and its measurement[J];Journal of Clinical Dermatology;2003-09
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