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《Journal of Clinical Neurology》 2006-05
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Applying Lactacystin,a selective proteasome inhibitor,to set up the rat model of Parkinson's disease with Lewy body

ZHANG Ke-zhong,JIANG Yu-ping,DING Xin-sheng. Department of Neurology,the First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,China  
Objective To set up the Parkinson's disease(PD)rat model with pathologic characteristic of Lewy body in nigral cells.Methods SD rats were injected respectively with 8 mg Lactacystin(Lactacystin group),sodium saline(NS group)and 12 mg 6-OHDA(6-OHDA group)by stereotaxic unilateral injection into the pars compacta of substantia nigral.The spontaneous and apomorphine-induced contralateral behaviors of rats were observed.The changes of midbrain histology were viewed by microscope;expression of α-synuclein and tyrosine hydroxylase(TH)positive cells were investigated by immunohistochemistry.The contents of dopamine and homovanillic acid in striatum were determined.Results Rats of NS group did not display abnormal behavior.The animals treated with Lactacystin developed progressively bradykinesia,hypokinesia,tremor,contralateral head tilting,and displayed apomorphine-induced contralateral rotation behavior;3 weeks later the number of TH positive cells were decreased by 83.29% compared with NS group(P0.01),some of which contained Lewy body with intense immune response to α-synuclein.The contents of dopamine and homovanillic acid in Lactacystin group(154.82±37.17,98.66±18.81)were less than those of in NS group in striatum(all P0.01).The rats of 6-OHDA group displayed behavior similar to those of Lactacystin group;the number of nigral cells decreased significantly but no Lewy body.Conclusion The rat model of Parkinson's disease with Lewy body is set up by using Lactacystin which can block the α-synuclein degradation.
【CateGory Index】: R-332
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【Citations】
Chinese Journal Full-text Database 2 Hits
1 LU Jian-ming, ZHOU Hou-guang,BAO Yuan-cheng, et al. Department of Neurology, Affiliated Changshu Hospital of Medical College, Yangzhou University, Changshu 215500,China;Experimental study on cytotoxicity of levodopa in Parkinson's disease rat[J];Journal of Clinical Neurology;2004-05
2 DING Zheng tong,REN Hui min,JIANG Yu ping,CAI Zhen ling △ ,ZHU Qiu yu △△ (Department of Neurology, △△ Department of Diabetes Research,Huashan Hospital,Fudan University, Shanghai 200040; △Department of Medicine,Minhang H;Influence of Paraquat on the System of Substantial Nigra and Striatum in C57BL Mice[J];Journal of Shanghai Medica(University);2001-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Ying,CHEN Jianzong,HOU Rong-rong,et al(Institute of Traditional Chinese Medicine,Laboratory of Integrated Traditional Chinese Medicine andWestern Medicine on Elderly Encephalopathy,Xijing Hospital,Fourth Military Medical University,Xi'an,Shaanxi,710032);Neuroprotective effects of TSG on Degeneration of Nigral Dopaminergic Neurons Induced by Paraquat and Maneb in Mice[J];Journal of Chengdu University of Traditional Chinese Medicine;2008-01
2 LIU Wei-guo, CHEN Sheng-di,WANG Xi-jin, et al. Department of Neurology, Clinic & Research Center for Parkinson's Disease, Ruijin Hospital, Shanghai Jiaotong University, Shanghai 200025, China;Cytotoxicity of Levodopa on c17.2 neural stem cell and neuroprotection of Pergolide[J];Journal of Clinical Neurology;2007-02
3 REN Jin Peng,REN Hui Min,JIANG Yu Ping Department of Neurology,Huashan Hospital, Fudan University, Shanghai 200040,China;Decrease of Dopamine Transporter in Striatum of Parkinsonian Mice Induced by Paraquat[J];Chinese Journal of Clinical Neurosciences;2002-01
4 DING Zheng Tong, REN Hui Min,JIANG Yu Ping , et al. Department of Neurology, Huashan Hospital, Fudan University, Shanghai 200040,China;Adult C57b1 Mice Could Develop Symptoms Similar to Parkinson′s Disease after the Exposure to Paraquat[J];Chinese Journal of Clinical Neurosciences;2002-03
5 DING Zheng-Tong, REN Hui-Min, JIANG Yu-Ping Department of Neurology, Huashan Hospital, Fudan University, Shanghai 200040,China;Apoptosis of Substantia Nigra Dopaminergic Neurons in C57BL Mice Exposed to Paraquat for Different Periods[J];Chinese Journal of Clinical Neurosciences;2003-01
6 DING Zheng-Tong, JIANG Yu-Ping ▲,REN Hui-Min,LU Yuan  Department of Neurology, Department of Laboratory Medicine Huashan Hospital, Fudan University, Shanghai 200040,China;The Influence of Paraquat on Cerebral Mitochondrial Respiratory Chain Activity After Mice Exposed to Paraquat by Oral Administration[J];Chinese Journal of Clinical Neurosciences;2003-03
7 ZHANG Ke-Zhong, JIANG Yu-Ping, WANG Jian,et al Department of Neurology, People' s Hospital of Jiangsu Province, Nanjing 210029; Department of Neurology, Huashan Hospital, Fudan University, Shanghai 200040, China;Synergistic Toxic Effect of Paraquat and Lactacystin on Substantia Nigra[J];Chinese Journal of Clinical Neurosciences;2005-01
8 REN Jin-Peng 1,2 , REN Hui-Min 1* , JIANG Yu-Ping 1* . 1. Department of Neurology, Fudan University Affiliated Huashan Hospital, Shanghai 200040, 2.Department of Neurology, Shanghai Jiaotong University Affiliated Sixth People's Hospital,Shanghai 200232,China;Selective Damage of Paraquat on Dopaminergic Neurons in Substanti Nigra of Mice[J];Chinese Journal of Clinical Neurosciences;2005-03
9 ZHANG Nan,CHEN Xin,ZHANG Si-yu;Research of Parkinson's disease model treated by Paraquat on C57BL Mice[J];Journal of Brain and Nervous Diseases;2008-02
10 REN Jin-peng1,SUN Xiao-jiang1,JIANG Yu-ping2 (1.Department of Neurology,The Sixth People's Hospital,Shanghai Jiaotong University,Shanghai 200233,China;2.Department of Neurology,Huashan Hospital,Fudan University,Shanghai 200040,China);Influence of paraquat on oxidative stress of dopaminergic neuron in substantia nigra of mice[J];Journal of Shanghai Jiaotong University(Medical Science);2007-01
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 LI Gang,CHEN Jian zong,LIANG Hong juan,ZHANG He long,JI Tong li (Department of Oncology,Tangdu Hospital,the Fourth Military Medical University,PLA,Shaanxi Xi′an 710032,China);Effect of Peibu Ganshen Recipe on Changes of Behavior and Nigra Monoamine Transmitters of Brain in Mice with Parkinson′s Disease[J];Journal of Anhui Traditional Chinese Medical College;2003-06
2 PU Xiao Ping 1, LI Xiao Rong 1, LI Hui Nong 1, TU Peng Fei 2, SONG Zhi Hong 2, LI Chang Ling 1 (1. Department of Molecular and Cellular Pharmacology, 2. Department of Natural Medicines, Peking University School of Pharmaceutical Sciences, B;Campneoside Ⅱ of Cistanche tubulosa (Schenk) R. Wight protects neurons from apoptosis induced by neurotoxin 1 methyl 4 phenylpyridinium (MPP +)[J];Journal of Beijing Medical University;2001-03
3 WANG Xue Mei △, FU Hong, LIU Geng Xin (Institute of Integrated of Traditional Chinese and Western Medicine, Peking University, Beijing 100034,China);Effect of Herba Epimedii and Fructus Lycii on mitochondrial DNA deletion, activity of respiratory chain enzyme complexes and ATP synthesis in aged rats[J];Journal of Beijing Medical University;2002-01
4 JIANG Yan,SHEN Min,ZHAO Ziqin,et al./ △Fudan University,Shanghai 200032;The distribution of methamphetamine and its metabolite amphetamine in guinea pig's tissues[J];Chinese Journal of Forensic Medicine;2002-01
5 YIN Feng,TIAN Zeng-min,ZHAO Quan-jun,WANG Ya-ming,YU Xue,LIU Shuang(Department of Neurosurgery,Naval General Hospital,Beijing 100037,China);Experimental Study on Establishment of Rats Model of Parkinson Disease by 6-OHDA Injection[J];Journal of Naval General Hospital;2007-02
6 Dou Yongqi,Yang Minghui,Liu Yi et al . General Hospital of PLA,Beijing 100853,China;Study on the mechanism of therapeutic effect of "Yichantang" in the treatment of Parkinson disease[J];Medical Journal of Chinese People's Liberation Army;2004-08
7 Li Xuefeng1, 2, Qiu Pingming1, Jin He1, Liu Ming1, Wang Huijun1△ (1 School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515; 2 Kunming General Hospital, Kunming 650032, China);Neurotoxicity of methamphetamine on related brain regions in rats[J];Chinese Journal of Anatomy;2007-01
8 ZHU Weifeng, LUO Rongjing, ZHOU Liping, TAN Bingyan (Guangzhou Municipal Hospital of TCM, Guangzhou 510130, China; Dept. of Physiology, Guangzhou University of TCM, Guangzhou 510405, China; Shenzhen Health Bureau, Shenzhen 518000 Guangdong, China; Central Laboratory of Preventive Medicine, College of Medicine, Zhongshan University, Guangzhou 510089, China);Experimental Studies on Zhenchan Ning for Parkinson's Disease[J];Journal of Guangzhou University of Traditional Chinese Medicine;2004-04
9 REN Jin-peng1,SUN Xiao-jiang1,JIANG Yu-ping2 (1.Department of Neurology,The Sixth People's Hospital,Shanghai Jiaotong University,Shanghai 200233,China;2.Department of Neurology,Huashan Hospital,Fudan University,Shanghai 200040,China);Influence of paraquat on oxidative stress of dopaminergic neuron in substantia nigra of mice[J];Journal of Shanghai Jiaotong University(Medical Science);2007-01
10 Shi Ganggang, Zhuang Xuexuan, Chen Jinxiang, Lin Lngping(Department of Pharmacology, Shantou University Medical College, 515031);A Convenience Model of Parkinson's Disease in Mice[J];JOURNAL OF SHANTOU UNIVERSITY MEDICAL COLLEGE;1999-01
【Secondary Citations】
Chinese Journal Full-text Database 4 Hits
1 Xiao Qin, Weng Zhongfang, Zhang Jing, et al.Department of Neurology, Ruijin Hospital, Shanghai Second Medical University,Shanghai 200025,China;Effects of levodopa on the serum excitatory amino acids and anti-oxidative indexes in rats with Parkinsonism[J];Journal of Clinical Neurology;2003-02
2 WANG Jian,CAI Zhen lin,REN Hui min,LU Chuan zhen,JIANG Yu ping (Department of Neurology,Huashan Hospital,Shanghai Medical University,Shanghai 200040);Damage of Paraquat and Dieldrin on Cultured Rat Dopaminergic Neurons[J];JOURNAL OF SHANGHAI MEDICA(UNIVERSITY);2000-04
3 Xu Xiurong,Tang Qinmei, Shanghai Institute of Materia Medica, Academia Sinica, Chen Shengdi, Xu Dilong and Yu Huizhen, Department of Neuro-logy, Ruijin Hospital,Shanghai ,Shanghai Second Medical University;Determination of Dopamine and Its Metabolites In the Brain of Parkinsonian Monkey by High Performance lonpair Liquid Chromatography[J];Chinese Journal of Chromatography;1988-02
4 ZHOU Hou-guang ,LU Jian-ming,BAO Yuan-cheng,et al. The Department of Neurology,The 2nd Hospital of Changsu,Jiang shu 215500;The establishment and evaluation of 6-OHDA-lesioned rat model for parkinson's disease[J];Chinese Journal of Behavioral Medical Science;2002-01
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