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《Journal of Clinical Neurology》 2009-04
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Study of N-methyl-D-aspartic acid receptor 2B level and calcium/calmodulin-dependent protein kinase Ⅱ activities in the hippocampus of vascular dementia rats and the intervention effects of Memantine

HE Yu,ZHAO Hang,ZHANG Yu.Department of Electrodiagnosis,the Second Hospital of Jilin University,Changchun 130041,China  
Objective To investigate changes of N-methyl-D-aspartic acid receptor 2B(NR2B) level and calcium/calmodulin-dependent protein kinase Ⅱ(CaMK Ⅱ) activities in the hippocampus of vascular dementia(VD) rats and the intervention effects of Memantine.Methods The VD rat models were established by permanent,bilateral occlusion of the common carotid arteries.The rats were randomly divided into VD group and Memantine-treated group.At 4,8,12,16 weeks after operation,the water maze test was performed to detect the ability of learning and memory of the rats.The changes of NR2B level were measured by RT-PCR.The changes of the CaMK Ⅱ activities were determined by incorporation of 32P into histone.The resoults were compared with the sham-operated group.Results Compared with the sham-operated group,the ability of learning and memory of VD group rats at each time point after operation decreased significantly(all P0.01);the level of NR2B increased significantly at 4 weeks after operation(P0.05)and decreased at 8~16 weeks after operation(all P0.01).Total CaMK Ⅱ activity increased significantly at 4 weeks after operation(P0.01),then dropped at 8 weeks after operation,and decreased significantly at 12,16 weeks after operation(all P0.01).In Memantine-treated group,at 4 weeks after operation,there were statistical differences in the indexes mentioned above in contrast with the VD group(all P0.01),and no statistical differences with the sham group.However,at other time point,there were significant differences with sham group(P0.05~0.01).Moreover,the ability of learning and memory and the level of NR2B were greatly better than those of VD group(P0.05~0.01) at 8,12 weeks after operation but totle CaMK Ⅱ activitiy were similar to those of VD group.Conclusions NR2B level and CaMK Ⅱ activities in the hippocampus of VD rats decrease and Memantine can improve VD rat's recognition by modulating the expression of NR2B,but its effect on CaMK Ⅱ activities is limited.
【CateGory Index】: R749.161
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