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Puerarin decreases isoprenaline induced myocardial fibrosis and expression of connective tissue growth factor in myocardial tissue of rats

LIU Shiying WANG Menghong ZHENG Zeqi PENG JingtianPENG Xiaoping FU Yongnan(Department of Cardiology, the First Affiliated Hospital of Nanchang University, Nanchang, 330006, China)  
Objective:To investigate the effect of puerarin on isoprenaline (ISO) induced myocardial fibrosis and the expression of connective tissue growth factor (CTGF) in myocardial tissue in rats.Method:SD male rats were divided into 4 groups randomly: ①model group (group M, n=16), rat models of myocardial fibrosis were established by subcutaneous injection (s.c.) with ISO (0.05% ISO, 5 mg/kg per day for ten days); ②early treatment with puerarin group (group Pa, n=12), rats received intraperitoneal injection (i.p.) of peurarin (100 mg/kg per day for 56 days plus operation; ③later treatment with puerarin group (group Pp, n=12), rats received peurarin (100 mg/kg per day for 46 days starting from the 10th day after operation; ④control group (group C, n=8). Left ventricle (LV) weight to body weight ratio was assessed. The collagen volume fraction (CVF) in LV was measured after specific Van Gieson (VG) collagen staining. CTGF protein content of LV myocardiac tissue was evaluated by quantitative immunohistochemistry staining. CTGF mRNA level and hydroxyproline concentration in myocardiac tissue were measured.Result:In comparison with group C, Pa, Pp, and M groups had higher LV weight to body weight ratio, CVF, hydroxyproline concentration and CTGF levels in myocardiac tissue(P0.01). But among the 3 ISO-treated groups, all of the parameters are significantly lower in Pa and Pp groups than in group M, especially in group Pa.Conclusion:These results suggest that peurarin can decrease the excessive collagen deposition induced by ISO in rat especially in early treatment, mostly through suppressing the overexpression of CTGF in myocardial tissue.
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